Pro-inflammatory M1/Th1 type immune network and increased expression of TSG-6 in the eutopic endometrium from women with endometriosis

Matteo, Maria; Cicinelli, Ettore; Neri, Margherita; Carpagnano, Francesca Anna; Romeo, Franca; Scutiero, Gennaro; Greco, Pantaleo; Garlanda, Cecília; Vendemiale, Gianluigi; Levi-Setti, Paolo Emanuele; Serviddio, Gaetano

doi:10.1016/j.ejogrb.2017.08.014

Cited by 16 publications

(10 citation statements)

References 34 publications

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“…Aspects of decidualization in endometriosis can be characterized as overexpressed. Women with endometriosis demonstrate more mobile endometrial stromal cells, higher numbers of uterine natural killer cells, reduced regulation over endometrial invasion, as well as up‐regulated angiogenesis, pro‐inflammatory cytokine expression, and oxidative stress (Brosens et al, 2012 ; Gellersen & Brosens, 2014 ; Lessey et al, 2013 ; Matteo et al, 2017 ; Sharpe‐Timms, 2001 ; Soares et al, 2012 ; Xavier et al, 2005 ). Indeed, Somigliana et al ( 1999 ) hypothesized that immunological alterations to the endometria and other tissues of women with endometriosis promote excellent endometrial receptivity, in the absence of severe effects from endometriosis such as adhesions and anatomical obstructions.…”

Section: Resultsmentioning

confidence: 99%

Endometriosis and polycystic ovary syndrome are diametric disorders

Dinsdale

Crespi

2021

Evolutionary Applications

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Evolutionary and comparative approaches can yield novel insights into human adaptation and disease. Endometriosis and polycystic ovary syndrome (PCOS) each affect up to 10% of women and significantly reduce the health, fertility, and quality of life of those affected. PCOS and endometriosis have yet to be considered as related to one another, although both conditions involve alterations to prenatal testosterone levels and atypical functioning of the hypothalamic-pituitary-gonadal (HPG) axis. Here, we propose and evaluate the novel hypothesis that endometriosis and PCOS represent

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Section: Resultsmentioning

confidence: 99%

Endometriosis and polycystic ovary syndrome are diametric disorders

Dinsdale

Crespi

2021

Evolutionary Applications

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“…A striking aspect of the phenotypic changes we have observed with ME-SFCs is that these changes are maintained after weeks of culture from freshly obtained menstrual effluent, with no difference in the culture conditions for ME-SFCs from controls and endometriosis patients. Previous studies report that the eutopic endometrium of patients with endometriosis is in fact different from the eutopic endometrium of controls, with changes in gene expression, and the presence of inflammatory mediators, including TNF (12)(13)(14). Several cell types in the endometrium produce TNF, including stromal fibroblasts and epithelial cells (50)(51)(52).…”

Section: Discussionmentioning

confidence: 99%

Menstrual Effluent Provides a Novel Diagnostic Window on the Pathogenesis of Endometriosis

Nayyar

Saleem

Yılmaz

et al. 2020

Front. Reprod. Health

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Endometriosis is a chronic inflammatory disorder characterized by the presence of endometrial-like tissue growing outside of the uterus. Although the cause is unknown, retrograde menstruation leads to deposition of endometrial cells into the peritoneal cavity. Lack of disease recognition and long diagnostic delays (6-10 years) lead to substantial personal, social and financial burdens, as well as delayed treatment. A non-invasive diagnostic for endometriosis is a major unmet clinical need. Here, we assessed whether differences in menstrual effluent-derived stromal fibroblast cells (ME-SFCs) from women with and without endometriosis provide the basis for a non-invasive diagnostic for endometriosis. In addition, we investigated whether treatment of control ME-SFCs with inflammatory cytokines (TNF and IL-1β) could induce an endometriosis-like phenotype. ME-SFCs from laparoscopically diagnosed endometriosis patients exhibit reduced decidualization capacity, measured by IGFBP1 production after exposure to cAMP. A receiver operating characteristic (ROC) curve developed using decidualization data from controls and endometriosis subjects yielded an area under the curve of 0.92. In addition, a significant reduction in ALDH1A1 gene expression and increased podoplanin surface expression were also observed in endometriosis ME-SFCs when compared to control ME-SFCs. These endometriosis-like phenotypes can be reproduced in control ME-SFCs by exposure to inflammatory cytokines and are associated with increased cell migration. These results are consistent with the hypothesis that chronic intrauterine inflammation influences the development of endometriosis lesions following retrograde menstruation. In conclusion, the analysis of ME-SFCs can provide an accurate, rapid, and non-invasive diagnostic for endometriosis and insight into disease pathogenesis.

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“…Ovarian response was monitored by serial transvaginal scanning and hormonal monitoring. Gonadotrophin [12] dosage was further adjusted based on the ovarian response. When one to three leading follicles was ≥18 mm in diameter, 250 µg of human chorionic gonadotropin (hCG, Ovidrel, Merck Serono S.p.A.) was administered to trigger final maturation of the oocytes.…”

Section: Methodsmentioning

confidence: 99%

“…The reduced cytotoxic effect of NK cells can increase autoimmune reactivity, while the decreased T cell reactivity changes the Th1/Th2 balance toward Th2. Imbalanced Th1/Th2 may be related to endometriosis-associated infertility [12].…”

Section: Introductionmentioning

confidence: 99%

Effect of Endometrioma cystectomy on cytokines of follicular fluid and IVF outcomes

et al. 2019

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Background Endometriosis patients undergoing in vitro fertilization-embryo transfer (IVF-ET) treatment suffer from lower success rates. The success of IVF-ET is related to the receptivity of the uterus and the quality of embryos, and it is well known a patient’s endometriosis does not impair the receptivity. Whether endometrioma should be removed surgically before IVF remains controversial. Studies have shown that endometrioma removal decreases peritoneal inflammation, but little information is available regarding the alteration in the cytokines of follicular fluid. The objective of this study was to examine the impact of endometrioma cystectomy on the outcome of IVF and the levels of intrafollicular inflammatory cytokines and to investigate correlations between cytokine concentrations and IVF outcomes. Method A total of 41 women with endometriosis-associated infertility undergoing IVF were recruited; 13 patients (surgery group, S group) had surgery to remove the endometrioma before enrollment, and 28 patients (non-surgery group, NS group) were untreated before IVF. The follicular fluid from a dominant follicle was collected during oocyte retrieval, and the concentrations of sixteen soluble cytokines known to be involved in ovarian function were measured. Results Among the soluble molecules examined in this study, chemokines and growth factors and a few are inflammatory cytokines were found in the follicular fluid of patients with endometriosis. In addition, the expression levels of chemokines, growth factors, and most inflammatory cytokines did not differ between the S and NS groups, but interleukin (IL)-18 levels were significantly lower in the NS group. However, the levels of IL-18 in the FF did not correlate with IVF cycle parameters. The implantation and clinical pregnancy rates were similar between the two groups, but the anti-Müllerian hormone (AMH) level was lower in the S group than in the NS group. Conclusions These findings suggest that endometrioma surgery may potentially reduce the ovarian reserve and has little impact on the success rate of IVF. Ovarian endometriomas are not associated with cytokine profiles in FF from infertile women, and they are not likely to affect the quality of the oocyte and embryo as a result of an inflammatory mechanism.

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Pro-inflammatory M1/Th1 type immune network and increased expression of TSG-6 in the eutopic endometrium from women with endometriosis

Cited by 16 publications

References 34 publications

Endometriosis and polycystic ovary syndrome are diametric disorders

Endometriosis and polycystic ovary syndrome are diametric disorders

Menstrual Effluent Provides a Novel Diagnostic Window on the Pathogenesis of Endometriosis

Effect of Endometrioma cystectomy on cytokines of follicular fluid and IVF outcomes

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