Pro-oxidative versus antioxidative reactions between Trolox and Cr(VI): The role of H2O2

Poljšak, Borut; Gazdag, Zoltán; Pesti, Miklós; Jenko-Brinovec, Š.; Belágyi, Joseph; Plesničar, S.; Raspor, Peter

doi:10.1016/j.etap.2005.11.004

Cited by 11 publications

(3 citation statements)

References 20 publications

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“…Data presented in this study suggest the induction of apoptosis-like processes in the tested Candida spp. that may be due to the accumulation of reactive oxygen species (ROS) that induce or regulate the apoptosis in yeasts [23,24]. Using the superoxide and peroxide radicals' fluorescent assays, we showed the accumulation of reactive oxygen species, which are the indicators of lipid damage [25].…”

Section: Discussionmentioning

confidence: 96%

Cytotoxic Action of Artemisinin and Scopoletin on Planktonic Forms and on Biofilms of Candida Species

et al. 2020

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We investigated the antifungal activities of purified plant metabolites artemisinin (Ar) and scopoletin (Sc) including inhibition, effects on metabolic activities, viability, and oxidative stress on planktonic forms and on preformed biofilms of seven Candida species. The characteristic minimum inhibitory concentration (MIC90) of Ar and Sc against Candida species ranged from 21.83–142.1 µg/mL and 67.22–119.4 µg/mL, respectively. Drug concentrations causing ≈10% CFU decrease within 60 min of treatments were also determined (minimum effective concentration, MEC10) using 100-fold higher CFUs than in the case of MIC90 studies. Cytotoxic effects on planktonic and on mature biofilms of Candida species at MEC10 concentrations were further evaluated with fluorescent live/dead discrimination techniques. Candida glabrata, Candida guilliermondii, and Candida parapsilosis were the species most sensitive to Ar and Sc. Ar and Sc were also found to promote the accumulation of intracellular reactive oxygen species (ROS) by increasing oxidative stress at their respective MEC10 concentrations against the tested planktonic Candida species. Ar and Sc possess dose-dependent antifungal action but the underlying mechanism type (fungistatic and fungicidal) is not clear yet. Our data suggest that Ar and Sc found in herbal plants might have potential usage in the fight against Candida biofilms.

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Section: Discussionmentioning

confidence: 96%

Cytotoxic Action of Artemisinin and Scopoletin on Planktonic Forms and on Biofilms of Candida Species

et al. 2020

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“…As presented in Section 4.2, a substantial increase in total Cr accumulation by S. cerevisiae cells was achieved by increasing the reducing capacity of the cytosol with vitamin C or Trolox pretreatment [19,142]. The bioaccumulation of Cr(VI) could also be augmented in Sch.…”

Section: Impacts Of Chromium On Environmentbioremediation Practicesmentioning

confidence: 94%

“…As a consequence, the pretreatment of yeast cells with reductants resulted in a decreased cytotoxicity and genotoxicity of Cr(VI) [19,142]. O'Brien et al [21] observed that the intracellular reduction of Cr(VI) has a toxifying effect, and the rate of Cr(VI) reduction influences the kinetics and extent of cellular damage.…”

Section: Prevention Of Chromium-induced Damagementioning

confidence: 97%

Interference of chromium with biological systems in yeasts and fungi: a review

Poljšak

Pócsi

Raspor

et al. 2010

J. Basic Microbiol.

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This paper deals with the interactions of chromium (Cr) with biological systems, focusing in particular on yeasts and fungi. These interactions are analysed with primarily regard to biochemical functions, but higher levels of organization are also considered. Thus, the morphological and cytological characteristics of selected microorganisms in response to exposure to chromium ions are evaluated. The different oxidation states of chromium and reactive oxygen species (ROS) generated in redox reactions with chromium ions are presented and characterized. The interactions of the most exposed subcellular structures, including the cell wall, plasma membrane and nuclei, have been deeply investigated in recent years, for two major reasons. The first is the toxicity of chromium ions and their strong impact on the metabolism of many species, ranging from microbes to humans. The second is the still disputed usefulness of chromium ions, and in particular trivalent chromium, in the glucose and fat metabolisms. Chromium pollution is still an important issue in many regions of the world, and various solutions have been proposed for the bioremediation of soil and water with selected microbial species. Yeasts and especially moulds have been most widely investigated from this aspect, and the biosorption and bioaccumulation of chromium for bioremediation purposes have been demonstrated. Accordingly, the mechanisms of chromium tolerance or resistance of selected microbes are of particular importance in both bioremediation and waste water treatment technologies. The mechanisms of chromium toxicity and detoxification have been studied extensively in yeasts and fungi, and some promising results have emerged in this area.

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The antioxidant and pro‐oxidant activity of vitamin C and trolox in vitro: a comparative study

Poljšak

Raspor

2007

J of Applied Toxicology

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The antioxidant and pro-oxidant properties of ascorbic acid (vitamin C) and the water-soluble analogue of alpha-tocopherol (trolox) were compared. Trolox has advantages over alpha-tocopherol, the latter being only lipid-soluble due to the presence of a carboxyl group in lieu of a phytol chain which imparts trolox with water solubility. Trolox is used as a standard antioxidant in biochemical studies against which the antioxidant capacity of compounds is compared. Although ascorbic acid and tocopherols possess strong antioxidant properties, they might also exhibit pro-oxidant properties in the presence of free transition metals. Thus, reactions detailed in this study were performed in the presence of Cr(VI) in an effort to investigate the potential of ascorbic acid and trolox to generate hydroxyl radicals in a Fenton-like reaction. Results obtained were derived from reactions containing the same concentration of ascorbic acid and trolox under identical experimental conditions. Hydroxyl radical formation was observed in the reaction of Cr(VI) with ascorbic acid resulting from ascorbic acid auto-oxidation and H2O2 formation. Hydroxyl radical formation was only detected in the reaction mixture containing Cr(VI) and trolox following the addition of H2O2.

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Pro-oxidative versus antioxidative reactions between Trolox and Cr(VI): The role of H2O2

Cited by 11 publications

References 20 publications

Cytotoxic Action of Artemisinin and Scopoletin on Planktonic Forms and on Biofilms of Candida Species

Cytotoxic Action of Artemisinin and Scopoletin on Planktonic Forms and on Biofilms of Candida Species

Interference of chromium with biological systems in yeasts and fungi: a review

The antioxidant and pro‐oxidant activity of vitamin C and trolox in vitro: a comparative study

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