PRO-XAN process:  design and evaluation of a pilot plant system for the coagulation and separation of the leaf protein from alfalfa juice

Bickoff, E. M.; Spencer, Roland R.; Mottola, A. C.; Clark, J. Peter; Kohler, G. O.

doi:10.1021/jf60175a018

Cited by 14 publications

References 14 publications

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Industrial process proteomics: Alfalfa protein patterns during wet fractionation processing

Incamps¹,

Hély-Joly

Chagvardieff

et al. 2005

Biotech & Bioengineering

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A proteome reference map of major soluble proteins from Medicago sativa (alfalfa) leaves and stems has been established for the first time. Among 195 spots analyzed by mass spectrometry and N-terminal Edman sequencing, 117 spots were unambiguously identified, representing 87 different proteins. Of these 87 proteins, 13 proteins were directly identified from the partial genome of Medicago sativa, 30 from expressed sequenced tags (ESTs) of the model legume Medicago truncatula and 44 from closely relative species by a cross-species protein identification method. The proteome map of Medicago sativa was then set as a reference to study the major high protein content products that are generated during the wet fractionation process of alfalfa green biomass. Using two-dimensional electrophoresis, we studied the variation of the protein patterns at different steps of the industrial-scale process. We clearly show that the process induces significant changes including chemical modifications, proteolytic events, and heat-shock protein responses. Strikingly, a certain level of cellular regulation is conserved during biomass processing, as exemplified by the induction of some heat shock proteins. Finally, all the results obtained in this proteomic study may help to identify novel products and to improve process designs in alfalfa biomass plants.

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Industrial process proteomics: Alfalfa protein patterns during wet fractionation processing

Incamps¹,

Hély-Joly

Chagvardieff

et al. 2005

Biotech & Bioengineering

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Inclusion of gas in casein and leaf protein precipitate particles and its effect on particle density

Munro

Tan

1984

J. Chem. Technol. Biotechnol.

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Floating precipitates often encountered in laboratory and industrial protein precipitate handling operations include isoelectric casein precipitates, casein precipitates formed by salting-out with ammonium sulphate, and heat precipitated leaf (lucerne) protein. These were investigated by measuring water-free densities and the variation in particle moisture content with temperature, and for each type of protein precipitate the density of degassed particles was greater than the density of the suspending medium, though the density difference was always small. Floating protein precipitates were therefore caused by the inclusion of gas in precipitate particles. Gas bubbles are easily incorporated into protein solutions. Furthermore for many types of protein precipitate, the physicochemical changes used to cause protein precipitation also drive dissolved gas from solution. Calculations showed that the amount of gas evolved is sufficient to cause flotation of all the precipitate formed.

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Fraction I Protein and Other Products from Tobacco for Food

Wildman

Kwanyuen

1978

Photosynthetic Carbon Assimilation

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PRO-XAN process: design and evaluation of a pilot plant system for the coagulation and separation of the leaf protein from alfalfa juice

Cited by 14 publications

References 14 publications

Industrial process proteomics: Alfalfa protein patterns during wet fractionation processing

Industrial process proteomics: Alfalfa protein patterns during wet fractionation processing

Inclusion of gas in casein and leaf protein precipitate particles and its effect on particle density

Fraction I Protein and Other Products from Tobacco for Food

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