Proanthocyanidins from the bark of Metasequoia glyptostroboides ameliorate allergic contact dermatitis through directly inhibiting T cells activation and Th1/Th17 responses

Chen, Fengyang; Ye, Xiaodi; Yang, Yongyi; Teng(, Tianli; Li, Xiaoyü; Xu, Shijun; Ye, Yiping

doi:10.1016/j.phymed.2015.03.006

Cited by 25 publications

(17 citation statements)

References 16 publications

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“…The plates were incubated for 48 h. The cells were collected and stained with FITC-anti-CD3. After washed with PBS, samples were immediately analyzed by a FACScan flow cytometer (Chen et al, 2015). …”

Section: Materials and Methodsdsmentioning

confidence: 99%

“…After incubation for 6 and 24 h, the cells were collected and stained with FITC-anti-CD3 plus PE-anti-CD69 and FITC-anti-CD3 plus PE-anti-CD25, respectively. After washing with PBS, the samples were immediately analyzed in a FACScan flow cytometer, respectively (Chen et al, 2015). …”

Section: Materials and Methodsdsmentioning

confidence: 99%

“…Primer amplification efficiency and specificity were verified for each set of primers. The mRNA expression levels of the tested genes relative to GAPDH were determined using the 2ΔΔCt method (Chen et al, 2015) and as fold induction.…”

Section: Materials and Methodsdsmentioning

confidence: 99%

“…As a consequence, the mining of novel T-cell targeting immunosuppressants from the pool of naturally occurring micro-molecules is of great interest. In previous studies, we have isolated dozens of natural products from plants that exhibited immunosuppressive activities both in vitro and in vivo (Ye et al, 2006, 2008; Chen et al, 2010, 2012, 2015). However, most of these natural products lacked the desired selective properties.…”

Section: Introductionmentioning

confidence: 99%

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A Novel Derivative of (-)mycousnine Produced by the Endophytic Fungus Mycosphaerella nawae, Exhibits High and Selective Immunosuppressive Activity on T Cells

Wang

Chen

et al. 2017

Front. Microbiol.

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An endophytic fungus, Mycosphaerella nawae ZJLQ129, was isolated from the leaves of the traditional Chinese medicine Smilax china. From the fermentation broth and mycelium, a dibenzofurane compound (-)mycousnine (1) was isolated. Chemical modification of it to the amide derivative (-)mycousnine enamine (2), which is new to science, was found to have high and selective immunosuppressive activity: similar to cyclosporin A, (-)mycousnine enamine (2) selectively inhibited T cell proliferation, suppressed the expression of the surface activation antigens CD25 and CD69 and the formation and expression of the cytokines interleukin-2 as well as interferon γ in activated T cells, but did not show any effect on the proliferation of B cells and cancer cells (PANC-1 and A549) and the activation of macrophages. Furthermore, the cytotoxicity of (-)mycousnine enamine was lower than that of cyclosporin A, and its therapeutic index (TC50/EC50) was 4,463.5, which is five-fold higher than that of cyclosporin A. We conclude that (-)mycousnine enamine (2), the semi-synthestic product prepared from the native product (-)mycousnine (1) of the endophyte M. nawae is a novel effective immunosuppressant showing low toxicity and high selectivity.

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Section: Materials and Methodsdsmentioning

confidence: 99%

Section: Materials and Methodsdsmentioning

confidence: 99%

Section: Materials and Methodsdsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Novel Derivative of (-)mycousnine Produced by the Endophytic Fungus Mycosphaerella nawae, Exhibits High and Selective Immunosuppressive Activity on T Cells

Wang

Chen

et al. 2017

Front. Microbiol.

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“…Primer amplification efficiency and specificity were verified for each set of primers. The mRNA expression levels of the tested genes relative to GAPDH were determined using the 2 ΔΔ C t method and showed as fold induction …”

Section: Methodsmentioning

confidence: 99%

The Loss of a Sugar Chain at C(3) Position Enhances Stemucronatoside K‐Induced Apoptosis, Cell Cycle Arrest, and Hedgehog Pathway Inhibition in HT‐29 Cells

Xiao-fang

Zhou

Yang

et al. 2016

Chemistry & Biodiversity

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Stemucronatoside K (SMK) and its aglycone stephanthraniline A (STA) are the most representative of a series of novel C steriodal compounds that we have previously isolated from Asclepiadaceae plants. The objectives of this study were to investigate the antitumor activity of SMK and STA, and clarify the effect of the sugar chain at the C(3) position. Our results showed that both SMK and STA decreased the growth of HT-29 cells in a dose- and time-dependent manner. Meanwhile, STA showed much stronger inhibitory effect than SMK. Treatment of HT-29 cells with STA increased the apoptotic cell numbers and the protein expression of cleaved caspase 3 and cleaved-PARP. G1 phase cell cycle arrest and decreased expression of cyclin D1 and cyclin-dependent kinases 4 were also observed after STA treatment. Furthermore, STA reduced the mRNA levels of four Hedgehog pathway components (GLI1, GLI2, GLI3, and PTCH1) and suppressed Shh-induced Hedgehog pathway activation in a concentration-dependent manner. These results indicated that SMK and STA could inhibit the growth of HT-29 cells by inducing apoptosis, cell cycle arrest, and hedgehog pathway inhibition. The loss of sugar chain at C(3) position could enhance SMK's activity. This study is beneficial to understand the use of natural C steroids as antitumor lead compounds.

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Research progress on the pathogenesis of psoriasis and its small molecule inhibitors

Xia,

Li,

Long

et al. 2024

Archiv der Pharmazie

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Psoriasis is a prevalent chronic systemic immune disease characterized by T‐cellmediated hyperproliferation of keratinized cells. Among its various manifestations, plaque‐type psoriasis is the most common. Treatment options for psoriasis encompass topical medications, biological therapies, phototherapy techniques, and others. However, traditional treatments are associated with numerous side effects. In contrast, targeted therapy has garnered increasing attention due to its high selectivity, strong safety profile, and favorable therapeutic outcomes. Patients with psoriasis lesions exhibit elevated levels of proinflammatory cytokines compared with the general population. These proinflammatory cytokines have been implicated in mediating psoriasis pathogenesis by inducing keratinocyte proliferation through multiple signaling pathways within the body. This study will delve into the Janus kinase‐signal transducers and activators of transcription, phosphatidylinositol 3 kinase (PI3K)‐protein kinase B (PKB, also known as AKT), and nuclear factor Kappa‐light‐chain‐enhancer of activated B cells signaling pathways to elucidate their roles in mediating psoriasis pathogenesis. In addition, we will summarize potential targets relevant to the treatment of psoriasis and discuss the design and activity assessment of their inhibitors. It also provides new insights for further in‐depth study of psoriasis and development of novel molecularly targeted inhibitors.

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Proanthocyanidins from the bark of Metasequoia glyptostroboides ameliorate allergic contact dermatitis through directly inhibiting T cells activation and Th1/Th17 responses

Cited by 25 publications

References 16 publications

A Novel Derivative of (-)mycousnine Produced by the Endophytic Fungus Mycosphaerella nawae, Exhibits High and Selective Immunosuppressive Activity on T Cells

A Novel Derivative of (-)mycousnine Produced by the Endophytic Fungus Mycosphaerella nawae, Exhibits High and Selective Immunosuppressive Activity on T Cells

The Loss of a Sugar Chain at C(3) Position Enhances Stemucronatoside K‐Induced Apoptosis, Cell Cycle Arrest, and Hedgehog Pathway Inhibition in HT‐29 Cells

Research progress on the pathogenesis of psoriasis and its small molecule inhibitors

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