Probing membrane topology by high-resolution 1H–13C heteronuclear dipolar solid-state NMR spectroscopy

Lü, Junxia; Damodaran, Krishnan; Lorigan, Gary A.

doi:10.1016/j.jmr.2005.09.006

Cited by 26 publications

(23 citation statements)

References 20 publications

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“…In addition, a 2D correlation spectrum of 13 C chemical shift and 13 C– 31 P dipolar couplings was acquired, which reports on the conformation of the phosphocholine headgroup region of DMPC. Similar results were obtained with a solution-state NMR spectrometer using a SAMMY experiment [182]. In a subsequent study, experimental parameters were optimized to maximize sensitivity and resolution [180].…”

Section: Separated Local Field (Slf) Nmr Studies On Bicellesmentioning

confidence: 72%

When detergent meets bilayer: Birth and coming of age of lipid bicelles

Dürr

Soong

Ramamoorthy

2013

Progress in Nuclear Magnetic Resonance Spectroscopy

119

108

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Section: Separated Local Field (Slf) Nmr Studies On Bicellesmentioning

confidence: 72%

When detergent meets bilayer: Birth and coming of age of lipid bicelles

Dürr

Soong

Ramamoorthy

2013

Progress in Nuclear Magnetic Resonance Spectroscopy

119

108

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“…The detection of 14 N nuclei could be used as a 'voltmeter' to study ligand-membrane interactions. We believe that the results presented in this paper can be utilized in performing advanced multidimensional solid-state NMR experiments [63][64][65] on bicelles containing peptides, proteins, drugs or other types of molecules.…”

Section: Discussionmentioning

confidence: 94%

Sensitivity and resolution enhancement in solid-state NMR spectroscopy of bicelles

Dvinskikh

Yamamoto

Dürr

et al. 2007

Journal of Magnetic Resonance

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Magnetically aligned bicelles are becoming attractive model membranes to investigate the structure, dynamics, geometry, and interaction of membrane-associated peptides and proteins using solution- and solid-state NMR experiments. Recent studies have shown that bicelles are more suitable than mechanically aligned bilayers for multidimensional solid-state NMR experiments. In this work, we describe experimental aspects of the natural abundance (13)C and (14)N NMR spectroscopy of DMPC/DHPC bicelles. In particular, approaches to enhance the sensitivity and resolution and to quantify radio-frequency heating effects are presented. Sensitivity of (13)C detection using single pulse excitation, conventional cross-polarization (CP), ramp-CP, and NOE techniques are compared. Our results suggest that the proton decoupling efficiency of the FLOPSY pulse sequence is better than that of continuous wave decoupling, TPPM, SPINAL, and WALTZ sequences. A simple method of monitoring the water proton chemical shift is demonstrated for the measurement of sample temperature and calibration of the radio-frequency-induced heating in the sample. The possibility of using (14)N experiments on bicelles is also discussed.

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“…At least three possible occurrences can explain this change in CSA line width, (1) increased stochastic fluctuations experienced by the lipid head groups due to an increase in the disorder of individual lipids caused by the increasing presence of the APH, but not the control, peptide; (2) increased axial rotational motion as well as lateral diffusion rate of lipid upon peptide incorporation; or (3) increased dynamics of the vesicles, resulting from the interaction between the peptide and head groups of the phospholipids [13, 32–34]. However, an increase in the long axis rotation of the lipid would be unlikely to affect the CSA line width because at the point at which the 31 P CSA line width becomes axially symmetric it has reached an average and increasing the rotation at that point will not change those averages.…”

Section: Resultsmentioning

confidence: 99%

“…All spectra display overlapping doublet resonances with the largest splitting resulting from the C-D bond closest to the glycerol backbone and the central splitting arising from the terminal methyl group [13, 31, 34]. Clearly, addition of APH peptide at various concentrations altered the line shape and resolution of 2 H NMR spectra of the POPC/POPC-d 31 MLVs significantly compared to the control sample lacking peptide (Figure 3A).…”

Section: Resultsmentioning

confidence: 99%

Investigating the interaction between peptides of the amphipathic helix of Hcf106 and the phospholipid bilayer by solid-state NMR spectroscopy

Zhang

Liu

Lorigan

et al. 2014

Biochimica et Biophysica Acta (BBA) - Biomembranes

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The chloroplast twin arginine translocation (cpTat) system transports highly folded precursor proteins into the thylakoid lumen using the protonmotive force as its only energy source. Hcf106, as one of the core components of the cpTat system, is part of the precursor receptor complex and functions in the initial precursor-binding step. Hcf106 is predicted to contain a single amino terminal transmembrane domain followed by a Pro-Gly hinge, a predicted amphipathic α-helix (APH), and a loosely structured carboxy terminus. Hcf106 has been shown biochemically to insert spontaneously into thylakoid membranes. To better understand the membrane active capabilities of Hcf106, we used solid-state NMR spectroscopy to investigate those properties of the APH. In this study, synthesized peptides of the predicted Hcf106 APH (amino acids 28–65) were incorporated at increasing mol% into 1-palmitoyl-2-oleoyl-sn-glycero-phosphocholine (POPC) and POPC/MGDG (monogalactosyldiacylglycerol; mole ratio 85:15) multilamellar vesicles (MLVs) to probe the peptide-lipid interaction. Solid-state 31P NMR and 2H NMR spectroscopic experiments revealed that the peptide perturbs the headgroup and the acyl chain regions of phospholipids as indicated by changes in spectral lineshape, chemical shift anisotropy (CSA) line width, and 2H order SCD parameters. In addition, the comparison between POPC MLVs and POPC/MGDG MLVs indicated that the lipid bilayer composition affected peptide perturbation of the lipids, and such perturbation appeared to be more intense in a system more closely mimicking a thylakoid membrane.

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Probing membrane topology by high-resolution 1H–13C heteronuclear dipolar solid-state NMR spectroscopy

Cited by 26 publications

References 20 publications

When detergent meets bilayer: Birth and coming of age of lipid bicelles

When detergent meets bilayer: Birth and coming of age of lipid bicelles

Sensitivity and resolution enhancement in solid-state NMR spectroscopy of bicelles

Investigating the interaction between peptides of the amphipathic helix of Hcf106 and the phospholipid bilayer by solid-state NMR spectroscopy

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