Probing sulfatide-tissue lectin recognition with functionalized glycodendrimersomes

Murphy, Paul V.; Romero, Antonio; Xiao, Qi; Ludwig, Anna‐Kristin; Jogula, Srinivas; Шилова, Н. В.; Singh, T. N.; Gabba, Adele; Javed, Bilal; Zhang, Dapeng; Medrano, F.J.; Kaltner, Herbert; Kopitz, Jürgen; Bovin, Nicolai V.; Wu, Albert M.; Klein, Michael L.; Percéc, Virgil; Gabius, Hans‐Joachim

doi:10.1016/j.isci.2020.101919

Cited by 19 publications

(16 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The emerging correlations between lectin expression and cellular processes will inspire functionally oriented studies, using cell biological test systems up to tissue cultures and animal models in healthy and diseased states. Synthetic surface programming of vesicle-like nanoparticles complements this toolbox by allowing to rationally manipulate glycan structures and their local density for testing [ 20 , 76 ]. In parallel, the application of labeled endogenous lectins, in analogy to plant lectin cyto- and histochemistry, can enormously help to give distinct aspects of the glycophenotype a physiological meaning.…”

Section: Discussionmentioning

confidence: 99%

What Cyto- and Histochemistry Can Do to Crack the Sugar Code

Habermann

Kaltner²,

Higuero

et al. 2021

Acta Histochem. Cytochem.

Self Cite

View full text Add to dashboard Cite

As letters form the vocabulary of a language, biochemical 'symbols' (the building blocks of oligo-and polymers) make writing molecular messages possible. Compared to nucleotides and amino acids, sugars have chemical properties that facilitate to reach an unsurpassed level of oligomer diversity. These glycans are a part of the ubiquitous cellular glycoconjugates. Cyto-and histochemically, the glycans' structural complexity is mapped by glycophenotyping of cells and tissues using receptors ('readers', thus called lectins), hereby revealing its dynamic spatiotemporal regulation: these data support the concept of a sugar code. When proceeding from work with plant (haem)agglutinins as such tools to the discovery of endogenous (tissue) lectins, it became clear that a broad panel of biological meanings can indeed be derived from the sugar-based vocabulary (the natural glycome incl. post-synthetic modifications) by glycan-lectin recognition in situ. As consequence, the immunocyto-and histochemical analysis of lectin expression is building a solid basis for the steps toward tracking down functional correlations, for example in processes leading to cell adhesion, apoptosis, autophagy or growth regulation as well as targeted delivery of glycoproteins. Introduction of labeled tissue lectins to glycan profiling assists this endeavor by detecting counterreceptor(s) in situ. Combining these tools and their applications strategically will help to take the trip toward the following long-range aim: to compile a dictionary for the glycan vocabulary that translates each message (oligosaccharide) into its bioresponse(s), that is to crack the sugar code.

show abstract

Section: Discussionmentioning

confidence: 99%

What Cyto- and Histochemistry Can Do to Crack the Sugar Code

Habermann

Kaltner²,

Higuero

et al. 2021

Acta Histochem. Cytochem.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Saccharides can also be linked to sphingolipids, yielding the broad class of glycosphingolipids (GSLs). The process starts with addition of a monosaccharide to a ceramide moiety, and such a small headgroup is already bioactive [61][62][63]. The sequential addition of monosaccharides leads to the formation of GSLs [64].…”

Section: Protein and Lipid Glycosylationmentioning

confidence: 99%

Glycans in autophagy, endocytosis and lysosomal functions

et al. 2021

Self Cite

View full text Add to dashboard Cite

Glycans have been shown to function as versatile molecular signals in cells. This prompted us to look at their roles in endocytosis, endolysosomal system and autophagy. We start by introducing the cell biological aspects of these pathways, the concept of the sugar code, and provide an overview on the role of glycans in the targeting of lysosomal proteins and in lysosomal functions. Moreover, we review evidence on the regulation of endocytosis and autophagy by glycans. Finally, we discuss the emerging concept that cytosolic exposure of luminal glycans, and their detection by endogenous lectins, provides a mechanism for the surveillance of the integrity of the endolysosomal compartments, and serves their eventual repair or disposal.

show abstract

“…It is noteworthy that each of these processes is highly specific and attributed to pairing with cell type-dependent counter-receptors [ 153 , 154 ]. Even a small sugar headgroup, such as that presented by sulfatide, can establish the contact site which makes ‘reading’ possible [ 155 ]. For example, long chains of N-acetyllactosamine (polyLacNAc) have high ligand capacity for distinct galectins [ 156 ], and for the Gal-3 CRD even allows for clustering with the possibility for stable contacts [ 157 ].…”

Section: Galectin Functionmentioning

confidence: 99%

The marriage of chemokines and galectins as functional heterodimers

Hundelshausen

Wichapong

Gabius

et al. 2021

Cell. Mol. Life Sci.

Self Cite

View full text Add to dashboard Cite

Trafficking of leukocytes and their local activity profile are of pivotal importance for many (patho)physiological processes. Fittingly, microenvironments are complex by nature, with multiple mediators originating from diverse cell types and playing roles in an intimately regulated manner. To dissect aspects of this complexity, effectors are initially identified and structurally characterized, thus prompting familial classification and establishing foci of research activity. In this regard, chemokines present themselves as role models to illustrate the diversification and fine-tuning of inflammatory processes. This in turn discloses the interplay among chemokines, their cell receptors and cognate glycosaminoglycans, as well as their capacity to engage in new molecular interactions that form hetero-oligomers between themselves and other classes of effector molecules. The growing realization of versatility of adhesion/growth-regulatory galectins that bind to glycans and proteins and their presence at sites of inflammation led to testing the hypothesis that chemokines and galectins can interact with each other by protein–protein interactions. In this review, we present some background on chemokines and galectins, as well as experimental validation of this chemokine–galectin heterodimer concept exemplified with CXCL12 and galectin-3 as proof-of-principle, as well as sketch out some emerging perspectives in this arena.

show abstract

Probing sulfatide-tissue lectin recognition with functionalized glycodendrimersomes

Cited by 19 publications

References 63 publications

What Cyto- and Histochemistry Can Do to Crack the Sugar Code

What Cyto- and Histochemistry Can Do to Crack the Sugar Code

Glycans in autophagy, endocytosis and lysosomal functions

The marriage of chemokines and galectins as functional heterodimers

Contact Info

Product

Resources

About