Probing the binding behavior and kinetic studies of amphiphilic acrylate copolymers with bovine serum albumin

Abstract

In this study, a series of copolymers poly(methyl methacrylate) (pMMA), poly(3-sulfopropyl methacrylate-co-methyl methacrylate) p(SPMA-co-MMA), and poly(dimethylaminoethyl methacrylate-co-methyl methacrylae) p(DMAEMA-co-MMA) were synthesized via free radical polymerization and the impact of surface charge on amphiphilic copolymers and its interactions with the bovine serum albumin (BSA) was studied. These amphiphilic copolymers are thermally stable at 100 oC and with Tg 82 and 132 oC. These copolymers pMD1 and pMS1 have -56.6 and -72.6 mV at pH 7.4 in PBS buffer solution that controls bovine serum albumin (BSA) adsorption capacity on polymers surface. AFM analysis further confirm minimum BSA film thickness of 0.474 and 0.324 nm for pMD1 and pMS1 as compared to pMMA. BSA adsorption for pMD1 and pMS1 was 0.9 and 1.5 mg/g while kinetic studies shown the suitability of best fitting of pseudo-first-order model that showed physisorption which was attained at pH 7.4 within 24 hours at 25oC.

Ultrafiltration polyanionic poly (3‐sulfopropyl methacrylate) membranes with enhanced antifouling and water flux

“…The results of the study depicted in Figure 7 showcase the efficacy of the improved membranes in rejecting BSA, which has a negative charge at pH 7 so do the blended membranes incorporated with PSPMA, this phenomenon enhanced the rejection of BSA protein. 26 The rejection rate of BSA by the unmodified (MVS0) was approximately 84%, however, for all modified membranes incorporated with PSPMA were found to be nearly 98%. The increased hydrophilic properties of the PES/PSPMA membranes, their wider pore diameters, and their improved overall membrane structure are responsible for this…”

“…Poly (3-Sulfopropyl methacrylate) (PSPMA) was synthesized as described in previous work. 26,27 Polyvinyl pyrrolidone (PVP) (MW = 29,000 g/mol), 1-methyl-2-pyrrolidone (NMP, 99%), ethyl acetate, dimethyl sulfoxide (99%), and ethanol were purchased from Sigma-Aldrich (St. Louis, MO). The aqueous solution was prepared by using deionized water.…”

“…Polyethersulfone (MW = 58,000 g/mol) was purchased from BASF company. Poly (3‐Sulfopropyl methacrylate) (PSPMA) was synthesized as described in previous work 26,27 . Polyvinyl pyrrolidone (PVP) (MW = 29,000 g/mol), 1‐methyl‐2‐pyrrolidone (NMP, 99%), ethyl acetate, dimethyl sulfoxide (99%), and ethanol were purchased from Sigma‐Aldrich (St. Louis, MO).…”