Probing the PEDOT:PSS/cell interface with conductive colloidal probe AFM-SECM

Knittel, Peter; Zhang, Hongrui; Kranz, Christine; Wallace, Gordon G.; Higgins, Michael J.

doi:10.1039/c5nr07155k

Cited by 32 publications

(33 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the field of coupled electrochemical methods, atomic force‐scanning electrochemical microscopy (AFM‐SECM) is commonly used for the in situ identification and structural characterization of individual virus particles and the mapping of protein distribution inside the same [126] . Knittel et al [127] . modified conductive colloid AFM‐SECM probes with polystyrene sulfonate and poly(3,4‐ethylenedioxythiophene) to use them for single‐cell force measurements in mouse fibroblasts and investigate single‐cell interactions under different applied electric potentials.…”

Section: Biospectroelectrochemistry Through Membranes and Cellsmentioning

confidence: 99%

In Situ and Operando Techniques for Investigating Electron Transfer in Biological Systems

et al. 2020

View full text Add to dashboard Cite

When bioelectrochemistry meets other analytical techniques for in situ experiments, new possibilities for understanding the nature of charge‐transfer reactions are provided. Despite progress in recent years in analytical instrumentation, a number of key issues remains for bioelectrochemistry, in terms of the mechanistic description of the surface reactions involved in biomolecules’ electron transfer. The main challenges of these techniques are the concomitant detection of electron transfer and molecular alteration as well as the development of suitable bioelectrodes. This review discusses the most recent and emerging in situ and operando electrochemical methods used to study biological systems such as redox proteins, nucleic acids, small biomolecules, cells, and biomembranes, focusing on electrochemical methods coupled with spectroscopic, spectrometric, and microscopic techniques.

show abstract

Section: Biospectroelectrochemistry Through Membranes and Cellsmentioning

confidence: 99%

In Situ and Operando Techniques for Investigating Electron Transfer in Biological Systems

et al. 2020

View full text Add to dashboard Cite

show abstract

“…AFM-SECM has been used to study biologically/biomedically relevant problems, i.e. mapping the flux of biomedically relevant electroactive molecules [ 128 ], detection of single-protein molecules at isolated viruses [ 127 , 135 ] and mapping adhesion properties of mouse fibroblasts using conductive polymer–modified colloidal AFM-SECM probes [ 136 ]. Recently, Daboss et al [ 137 ] investigated the adhesion of Pseudomonas fluorescens at the single bacterial cell level using polydopamine (PDA)-modified colloidal AFM-SECM probes.…”

Section: Future Potential Of Hybrid Secm Techniquesmentioning

confidence: 99%

Scanning electrochemical microscopy and its potential for studying biofilms and antimicrobial coatings

Caniglia

Kranz

2020

Anal Bioanal Chem

View full text Add to dashboard Cite

Biofilms are known to be well-organized microbial communities embedded in an extracellular polymeric matrix, which supplies bacterial protection against external stressors. Biofilms are widespread and diverse, and despite the considerable large number of publications and efforts reported regarding composition, structure and cell-to-cell communication within biofilms in the last decades, the mechanisms of biofilm formation, the interaction and communication between bacteria are still not fully understood. This knowledge is required to understand why biofilms form and how we can combat them or how we can take advantage of these sessile communities, e.g. in biofuel cells. Therefore, in situ and real-time monitoring of nutrients, metabolites and quorum sensing molecules is of high importance, which may help to fill that knowledge gap. This review focuses on the potential of scanning electrochemical microscopy (SECM) as a versatile method for in situ studies providing temporal and lateral resolution in order to elucidate cell-to-cell communication, microbial metabolism and antimicrobial impact, e.g. of antimicrobial coatings through the study of electrochemical active molecules. Given the complexity and diversity of biofilms, challenges and limitations will be also discussed.

show abstract

“…[42] This limitation and approaches to overcome such will be described in control, for instance, the working distance during scanning (e.g., SECM-atomic force microscopy (AFM), shear force, alternating current (AC)-SECM, among others). [54,[64][65][66][67][68][69] Alternatively, a new family of SECM probes, so-called soft stylus microelectrodes, has been developed recently to carry out SECM experiments of rough, tilted, and large substrates in contact mode without the need of any additional equipment ( Fig.1.11). [10,12,41,[70][71][72]…”

Section: Substrate Generation/tip Collection Modementioning

confidence: 99%

Soft Probes for Bio-electrochemical Imaging

Lin

2019

Springer Theses

View full text Add to dashboard Cite

Probing the PEDOT:PSS/cell interface with conductive colloidal probe AFM-SECM

Abstract: PSS) are used for single cell force measurements in mouse fibroblasts and single cell interactions are investigated as a function of the applied potential.

Cited by 32 publications

References 30 publications

In Situ and Operando Techniques for Investigating Electron Transfer in Biological Systems

In Situ and Operando Techniques for Investigating Electron Transfer in Biological Systems

Scanning electrochemical microscopy and its potential for studying biofilms and antimicrobial coatings

Soft Probes for Bio-electrochemical Imaging

Contact Info

Product

Resources

About