PROBIOTIC Bacillus subtilis AND Lactobacillus plantarum IN DIET OF NILE TILAPIA

Abstract

The aim of this study was to evaluate the effects of a probiotic, composed of Bacillus subtilis and Lactobacillus plantarum in Nile tilapia fry during the sex reversal phase under stress conditions caused by high stocking density. This experiment was conducted in the Fisheries Institute of São Paulo. The experiment design was completely randomized with four treatments: 1) probiotic added feed, 2) probiotic added to water, 3) probiotic added to feed and water and 4) control diet; with three replicates. The variables analyzed were: final weight, total length, specific growth rate, survival, intestinal microbiology and gene expression of TNF-α and HSP-70. The results of the zootechnical performance of growth and gene expression did not show significant differences between treatments in the parameters evaluated (P>0.05). In the intestinal tract of fry raised in water with added probiotic, log10 CFU (7.72 ± 0.51) count of Bacillus spp. was higher than with other treatments which themselves did not differ significantly. It was concluded that the addition of a probiotic of Bacillus subtilis and Lactobacillus plantarum in the feed or water of Nile tilapia fry during the sex reversal phase did not affect the zootechnical performance of growth or expression the genes studied, but modified the intestinal microbiota.

Oral administration of Bacillus subtilis and Lactobacillus plantarum modulates the gut microbiota and increases the amylase activity of Nile tilapia (Oreochromis niloticus)

“…The greater activity of maltase in Nile tilapia occurs in the gastric loop region so that most glucose release occurs in the medial portion of the intestine (Tengjaroenkul et al 2000). The distribution of maltase activity in the middle intestine of Nile tilapia is like other studies involving amylase activity that hydrolyzes starch into maltose or glucose (Evans 1998;Stevens and Hume 2004) which corroborates to the present work.…”

“…In a previous study, the inclusion of probiotics B. subtilis and L. plantarum in diets for Nile tilapia fry, during the sex reversal phase, modified the fish intestinal microbiota (Guimarães et al 2019). Therefore, the aim of this research was to evaluate the effects of feeding Nile tilapia (O. niloticus) with a diet containing 0.08% of a commercial probiotic AQUA-PHOTO ® [L. plantarum (1.51 × 10 6 CFU.g −1 ) and B. subtilis (1.34 × 10 7 CFU.g −1 )] on digestive enzymes' activity and gut microbiome profile.…”

Bacterial Isolated from <em>Oreochromis niloticus</em> and Possible Use as Probiotic

“…Therefore, the time of probiotic administration is an important factor to promote the beneficial effects, such as the production of digestive enzymes, inhibition of pathogenic bacteria, among others [35]. In this study, we verified significant difference in biomass between the 63rd and 84th days of the experiment.…”

Isolation and identification of bacteriocin-producing probiotic lactic acid bacteria from the rainbow trout (>i<Oncorhynchus mykiss>/i<): activity against >i<Flavobacterium psychrophilum>/i<

“…also observed higher survival of tilapia and digestive enzymes production, using a combination of different species of Bacillus at concentrations of 10 7 CFU/g and 10 8 CFU/g for a period of two weeks Dawood et al (2020a),Tan et al (2019). andAddo et al (2017a) also reported similar results administering probiotics at concentration in the range 10 6 -10 8 CFU/g, even though the treatment was prolonged for about 2 months in these studies Guimarães et al (2019. andAyyat et al (2014) included in their assays Lb.…”