Probiotic Enterococcus mundtii Isolate Protects the Model Insect Tribolium castaneum against Bacillus thuringiensis

Grau, Thorben; Vilcinskas, Andreas; Joop, Gerrit

doi:10.3389/fmicb.2017.01261

Cited by 52 publications

(44 citation statements)

References 96 publications

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“…The composition of the microbial community of an organism can be altered through rearing conditions (e.g. a probiotic diet), via a breeding regime or by genetic manipulation (Grau, Vilcinskas, & Joop, 2017; Ras et al ., 2017). For example, D. melanogaster fed a probiotic bacterium were less susceptible to infections of pathogenic bacteria (Blum et al ., 2013).…”

Section: How Can Genetics Be Used To Improve Biological Control?mentioning

confidence: 99%

Next‐generation biological control: the need for integrating genetics and genomics

et al. 2020

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Biological control is widely successful at controlling pests, but effective biocontrol agents are now more difficult to import from countries of origin due to more restrictive international trade laws (the Nagoya Protocol). Coupled with increasing demand, the efficacy of existing and new biocontrol agents needs to be improved with genetic and genomic approaches. Although they have been underutilised in the past, application of genetic and genomic techniques is becoming more feasible from both technological and economic perspectives. We review current methods and provide a framework for using them. First, it is necessary to identify which biocontrol trait to select and in what direction. Next, the genes or markers linked to these traits need be determined, including how to implement this information into a selective breeding program. Choosing a trait can be assisted by modelling to account for the proper agro‐ecological context, and by knowing which traits have sufficiently high heritability values. We provide guidelines for designing genomic strategies in biocontrol programs, which depend on the organism, budget, and desired objective. Genomic approaches start with genome sequencing and assembly. We provide a guide for deciding the most successful sequencing strategy for biocontrol agents. Gene discovery involves quantitative trait loci analyses, transcriptomic and proteomic studies, and gene editing. Improving biocontrol practices includes marker‐assisted selection, genomic selection and microbiome manipulation of biocontrol agents, and monitoring for genetic variation during rearing and post‐release. We conclude by identifying the most promising applications of genetic and genomic methods to improve biological control efficacy.

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Section: How Can Genetics Be Used To Improve Biological Control?mentioning

confidence: 99%

Next‐generation biological control: the need for integrating genetics and genomics

et al. 2020

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“…E. raffinosus which was reported to prevent bacterial infection in Labeo rohita and Labeo catla from E. coli, A. hydrophilla, S. aerous, S. typhimurium [43]. The identified E. hirae, reported producing lipase and bile salt hydrolase enzyme with antioxidant properties, and E. mundtii reported with antimicrobial activity [44]. Four Roseobacter spp., identified from the sediment metagenomes, were reported with therapeutic value for commercial aquaculture.…”

Section: Plos Onementioning

confidence: 95%

Metagenome analysis from the sediment of river Ganga and Yamuna: In search of beneficial microbiome

et al. 2020

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Beneficial microbes are all around us and it remains to be seen, whether all diseases and disorders can be prevented or treated with beneficial microbes. In this study, the presence of various beneficial bacteria were identified from the sediments of Indian major Rivers Ganga and Yamuna from nine different sites using a metagenomic approach. The metagenome sequence analysis using the Kaiju Web server revealed the presence of 69 beneficial bacteria. Phylogenetic analysis among these bacterial species revealed that they were highly diverse. Relative abundance analysis of these bacterial species is highly correlated with different pollution levels among the sampling sites. The PCA analysis revealed that Lactobacillus spp. group of beneficial bacteria are more associated with sediment sampling sites, KAN-2 and ND-3; whereas Bacillus spp. are more associated with sites, FAR-2 and ND-2. This is the first report revealing the richness of beneficial bacteria in the Indian rivers, Ganga and Yamuna. The study might be useful in isolating different important beneficial microorganisms from these river sediments, for possible industrial applications.

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“…Such bacteria are widely used as animal feed additives in food production [ 129 ]. Enterococcus mundtii bacteria isolated from the feces of Ephestia kuehniella have the function of protecting the flour beetle, Tribolium castaneum , against Bt infection [ 130 ]. The surface properties test showed that this isolate has intense levels of auto-aggregation, which is related to the formation of colonies in the host insect’s gut [ 131 ].…”

Section: The Interaction Between Bt and Host Gut Microbiotamentioning

confidence: 99%

“…Moreover, bacteria cell wall compounds, such as lipopolysaccharide (LPS) and peptidoglycan (PGN), have been well studied, and can stimulate the host immune response [ 132 , 133 ]. However, Tribolium castaneum larvae exposed to the corresponding supernatant can also increase the resistance to Bt infection [ 130 ]. This perhaps suggests that the protective function of probiotic bacteria is based on the secreted proteins or some small peptides, which may act directly against Bt infection or through the triggering of immune priming.…”

Section: The Interaction Between Bt and Host Gut Microbiotamentioning

confidence: 99%

The Tripartite Interaction of Host Immunity–Bacillus thuringiensis Infection–Gut Microbiota

Mandal

et al. 2020

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Bacillus thuringiensis (Bt) is an important cosmopolitan bacterial entomopathogen, which produces various protein toxins that have been expressed in transgenic crops. The evolved molecular interaction between the insect immune system and gut microbiota is changed during the Bt infection process. The host immune response, such as the expression of induced antimicrobial peptides (AMPs), the melanization response, and the production of reactive oxygen species (ROS), varies with different doses of Bt infection. Moreover, B. thuringiensis infection changes the abundance and structural composition of the intestinal bacteria community. The activated immune response, together with dysbiosis of the gut microbiota, also has an important effect on Bt pathogenicity and insect resistance to Bt. In this review, we attempt to clarify this tripartite interaction of host immunity, Bt infection, and gut microbiota, especially the important role of key immune regulators and symbiotic bacteria in the Bt killing activity. Increasing the effectiveness of biocontrol agents by interfering with insect resistance and controlling symbiotic bacteria can be important steps for the successful application of microbial biopesticides.

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Probiotic Enterococcus mundtii Isolate Protects the Model Insect Tribolium castaneum against Bacillus thuringiensis

Cited by 52 publications

References 96 publications

Next‐generation biological control: the need for integrating genetics and genomics

Next‐generation biological control: the need for integrating genetics and genomics

Metagenome analysis from the sediment of river Ganga and Yamuna: In search of beneficial microbiome

The Tripartite Interaction of Host Immunity–Bacillus thuringiensis Infection–Gut Microbiota

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