Probiotic Lactobacillus strains: in vitro and in vivo studies

Abstract

Three Lactobacillus strains (LOCK 0900, LOCK 0908, LOCK 0919) out of twenty-four isolates were selected according to their antagonistic activity against pathogenic bacteria, resistance to low pH and milieu of bile salts. Intragastric administration of a mixture of these strains to Balb/c mice affected cytokine T(H)1-T(H)2 balance toward nonallergic T(H)1 response. Spleen cells, isolated from lactobacilli-treated mice and re-stimulated in vitro with the mixture of heat-inactivated tested strains, produced significantly higher amounts of anti-allergic tumor necrosis factor- and interferon-gamma than control animals whereas the level of pro-allergic interleukin-5 was significantly lower. Lactobacillus cells did not translocate through the intestinal barrier into blood, liver and spleen; a few Lactobacillus cells found in mesenteric lymph nodes could create antigenic reservoir activating the immune system. The mixture of Lactobacillus LOCK 0900, LOCK 0908 and LOCK 0919 strains represents a probiotic bacterial preparation with possible use in prophylaxis and/or therapy of allergic diseases.

Fatty acid profile, color and lipid oxidation of organic fermented sausage during chilling storage as influenced by acid whey and probiotic strains addition

“…In vitro tests conducted on probiotic strains revealed the strains' resistance to low pH and bile salts (Cukrowska et al, 2009) and showed antagonistic activity against pathogenic bacteria. Moreover, the probiotic strains also synthesize bacteriocins resistant to proteolytic enzymes, e.g., biosurfactans with described antimicrobial function.…”

Colonization of germ-free mice with a mixture of three lactobacillus strains enhances the integrity of gut mucosa and ameliorates allergic sensitization

“…30 These selected strains showed properties required for probiotic bacteria, e.g., resistance to gastric acids and bile salts and inhibitory activities against bacterial pathogens. 30 Moreover, the mixture of these strains (Lmix) showed synergistic effects in the induction of anti-allergic Th1-type cytokines and regulatory cytokine TGF-b in human whole blood cell cultures compared with the levels induced by each single strain alone.…”

“…30 These selected strains showed properties required for probiotic bacteria, e.g., resistance to gastric acids and bile salts and inhibitory activities against bacterial pathogens. 30 Moreover, the mixture of these strains (Lmix) showed synergistic effects in the induction of anti-allergic Th1-type cytokines and regulatory cytokine TGF-b in human whole blood cell cultures compared with the levels induced by each single strain alone. 31 Our pilot study showed that the supplementation of children presenting the first symptoms of allergy (atopic dermatitis) with the Lmix reduced serum levels of IgE and IL-5 and diminished the severity of the disease (Cukrowska, unpublished data).…”

Lactobacillus gasseri OLL2809 and its RNA suppress proliferation of CD4+ T cells through a MyD88-dependent signalling pathway

“…37 The bacteria were found in the intracellular spaces of lymphocytes and macrophages in Peyer's patches. 36 Cukrowska et al reported that lactobacillus cells administered intragastrically did not translocate through the intestinal barrier into blood, liver and spleen, but were found alive in a mesenteric lymph node, although the localization of the bacteria in a mesenteric lymph node was not shown.…”