Probiotic potential and safety assessment of bacteriocinogenic Enterococcus faecium strains with antibacterial activity against Listeria and vancomycin-resistant enterococci

Fugaban, Joanna Ivy Irorita; Holzapfel, Wilhelm H.; Todorov, Svetoslav Dimitrov

doi:10.1016/j.crmicr.2021.100070

Cited by 23 publications

(12 citation statements)

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“…Many probiotics are LAB, and some of which can often be resistant to antibiotics, 76 and may represent a risk for ARG transmission if not carefully monitored. There are a variety of studies that focus on determining the safety of these widely used LAB probiotics, specifically Lactobacillus , 77–79 Bacillus , 64 and nonpathogenic Enterococcus , 80 with the purpose of identifying putative ARG or virulence-associated genes in commercial strains. Conjugative ARGs have indeed been identified in food-associated LAB.…”

Section: Expansion and Diminution Of The Gut Resistome By Live Microb...mentioning

confidence: 99%

Good microbes, bad genes? The dissemination of antimicrobial resistance in the human microbiome

et al. 2022

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A global rise in antimicrobial resistance among pathogenic bacteria has proved to be a major public health threat, with the rate of multidrug-resistant bacterial infections increasing over time. The gut microbiome has been studied as a reservoir of antibiotic resistance genes (ARGs) that can be transferred to bacterial pathogens via horizontal gene transfer (HGT) of conjugative plasmids and mobile genetic elements (the gut resistome). Advances in metagenomic sequencing have facilitated the identification of resistome modulators, including live microbial therapeutics such as probiotics and fecal microbiome transplantation that can either expand or reduce the abundances of ARG-carrying bacteria in the gut. While many different gut microbes encode for ARGs, they are not uniformly distributed across, or transmitted by, various members of the microbiome, and not all are of equal clinical relevance. Both experimental and theoretical approaches in microbial ecology have been applied to understand differing frequencies of ARG horizontal transfer between commensal microbes as well as between commensals and pathogens. In this commentary, we assess the evidence for the role of commensal gut microbes in encoding antimicrobial resistance genes, the degree to which they are shared both with other commensals and with pathogens, and the host and environmental factors that can impact resistome dynamics. We further discuss novel sequencing-based approaches for identifying ARGs and predicting future transfer events of clinically relevant ARGs from commensals to pathogens.

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Section: Expansion and Diminution Of The Gut Resistome By Live Microb...mentioning

confidence: 99%

Good microbes, bad genes? The dissemination of antimicrobial resistance in the human microbiome

et al. 2022

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“…Additionally, the current study indicates a higher abundance of Enterococcus species in rats fed CEL. Enterococcus faecalis improves host health [25,26] and is clinically relevant for the treatment of chronic recurrent bronchitis [27]. Some Enterococcus species are employed as probiotics and in the production of feed additives to prevent diarrhea in animals [24].…”

Section: Other Generamentioning

confidence: 99%

Aspergillus-Derived Cellulase Preparation Exhibits Prebiotic-like Effects on Gut Microbiota in Rats

et al. 2022

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Aspergillus-derived cellulase, which is involved in the production of Aspergillus-fermented foods, has been employed in the food and animal feed industries. However, the effect of dietary Aspergillus cellulase on health is poorly understood. Previously, we discovered that supplemental Aspergillus-derived protease and lipase preparations had substantial bifidogenic effects on rats fed a high-fat diet. Therefore, this study reports on the effects of a 0.1% dietary Aspergillus-derived cellulase preparation (CEL) on the gut microbiota of rats fed a high-fat diet. Gene sequencing analysis of 16S rRNA revealed that CEL treatment markedly affected the microbiota profiles of the cecal contents (p < 0.05). Notably, CEL markedly increased the relative abundance (RA) of typical probiotics, such as Bifidobacterium and Lactobacillus, at the genus level (28- and 5-fold, respectively, p < 0.05). Similarly, at the family level, CEL treatment significantly increased the RA of Bifidobacteriaceae and Lactobacillaceae (p < 0.05). Furthermore, CEL increased the RA of other genera, such as Collinsella and Enterococcus, but decreased the RA of Oscillospira, Dorea and Coprobacillus (p < 0.05). The effects on these genera are similar to those reported for typical prebiotic oligosaccharides. Overall, this study demonstrates the prebiotic-like effects of dietary CEL by significantly increasing Bifidobacterium and Lactobacillus abundance.

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“…Generally, E. faecium strains that possess hemolysin/cytolysin, virulence and/or vancomycin-resistance genes do not seem widespread in raw milk and traditional cheeses in Greece and, in recent years, few unsafe E. faecium strains were retrieved from various cheeses of neighboring countries ( Hammad et al., 2015 ; Özkan et al., 2021a ). The incidence of virulence factors in food isolates of E. faecium is strain-specific, like E. faecium ST7319ea ( Fugaban et al., 2021 ). In general, few strains within an isolated group, most of which belonged to E. faecalis , harbored the ace and gelE genes, as the E. faecalis KFM47, KFM48 and KFM50 strains ( Table 6 ), or the agg, IS16, espA and hyl- like genes ( Morandi et al., 2015 ; Hammad et al., 2015 ; Gaglio et al., 2016 ; Ruiz et al., 2016 ; Domingos-Lopez et al., 2017 ).…”

Section: Resultsmentioning

confidence: 99%

Antilisterial activity of raw sheep milk from two native Epirus breeds: Culture-dependent identification, bacteriocin gene detection and primary safety evaluation of the antagonistic LAB biota

Sioziou,

Kakouri,

Bosnea

et al. 2024

Current Research in Microbial Sciences

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Probiotic potential and safety assessment of bacteriocinogenic Enterococcus faecium strains with antibacterial activity against Listeria and vancomycin-resistant enterococci

Cited by 23 publications

References 85 publications

Good microbes, bad genes? The dissemination of antimicrobial resistance in the human microbiome

Good microbes, bad genes? The dissemination of antimicrobial resistance in the human microbiome

Aspergillus-Derived Cellulase Preparation Exhibits Prebiotic-like Effects on Gut Microbiota in Rats

Antilisterial activity of raw sheep milk from two native Epirus breeds: Culture-dependent identification, bacteriocin gene detection and primary safety evaluation of the antagonistic LAB biota

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