Probiotic Properties and Inhibitory Activity of Lactic Acid Bacteria Isolated from Vaginal Microbiota of Korean Women against Gardnerella vaginalis and Candida albicans

Kim, YongGyeong; Han, Sang‐Ik; Kim, Jin-Seong; Yulah, Jeong; Paek, Nam-Soo; Chang-Ho, Kang

doi:10.7841/ksbbj.2019.34.2.91

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“…Our previous in vivo studies demonstrated that five lactobacilli strains (Lacticaseibacillus paracasei MG4272, L. rhamnosus MG4288, Ligilactobacillus salivarius MG242, L. fermentum MG901, and Lactiplantibacillus plantarum MG989) isolated from the vaginal tract of healthy Korean women have good probiotic properties, such as acid/bile salt-resistance and adhesion (auto-aggregation). Furthermore, these strains demonstrated potential antimicrobial activity against GV or Candida albicans [14][15][16][17][18], and a three-strain mixture (MG242, MG901, and MG989) exhibited anti-BV activity in GV-infected mice [19].…”

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confidence: 99%

Lactobacilli Strain Mixture Alleviates Bacterial Vaginosis through Antibacterial and Antagonistic Activity in Gardnerella vaginalis-Infected C57BL/6 Mice

Choi

Won

Kim³

et al. 2022

Microorganisms

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The present study investigated the anti-bacterial vaginitis (BV) effects of a mixture of five lactobacilli strains (LM5), containing equal amounts of Ligilactobacillus salivarius MG242, Limosilactobacillus fermentum MG901, Lactiplantibacillus plantarum MG989, Lacticaseibacillus paracasei MG4272, and Lacticaseibacillus rhamnosus MG4288), in HeLa cells and Gardnerella vaginalis (GV)-infected BV mice. All strains produced lactic acid and hydrogen peroxide, and were resistant to nonoxynol-9. LM5 significantly inhibited GV growth by 80%, exhibited good adhesion to HeLa cells, and significantly inhibited GV adhesion to these cells. In GV-infected mice, LM5 administered orally at 5 × 109 CFU/mouse significantly inhibited GV proliferation in the vaginal tract and significantly reduced myeloperoxidase activity, pro-inflammatory cytokine (TNF-α, IL-1β, and IL-6) levels, and nitric oxide levels in vaginal tissue lysates. Histopathological analysis of vaginal tissues revealed that LM5 markedly suppressed the exfoliation of vaginal epithelial cells. Overall, these results suggest that LM5 might alleviate BV by direct antibacterial and antagonistic activity in vaginal tissues of GV-infected mice.

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