Process Development for Benzyl Alcohol Production by Whole-Cell Biocatalysis in Stirred and Packed Bed Reactors

Rodrigues, Carlos J. C.; Carvalho, Carla C. C. R. de

doi:10.3390/microorganisms10050966

Cited by 7 publications

(8 citation statements)

References 77 publications

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“…In the present study, a rock pond was used as sampling site because we have been able to find efficient biocatalysts in this type of marine environment ( Rodrigues et al, 2017 ; Rodrigues and de Carvalho, 2022 ), which is characterized by high salt concentration and UV-exposure. The aim of the study was 1) to compare the number of cells that could be grown under laboratorial conditions with the number of cells observed by fluorescence microscopy, and 2) to compare the isolates obtained by cultivation methods with those identified by metataxonomy.…”

Section: Introductionmentioning

confidence: 99%

Cultivating marine bacteria under laboratory conditions: Overcoming the “unculturable” dogma

Rodrigues

Carvalho²

2022

Front. Bioeng. Biotechnol.

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Underexplored seawater environments may contain biological resources with potential for new biotechnological applications. Metagenomic techniques revolutionized the study of bacterial communities but culture dependent methods will still be important to help the biodiscovery of new products and enzymes from marine bacteria. In this context, we promoted the growth of bacteria from a marine rock pond by culture dependent techniques and compared the results with culture independent methods. The total number of bacteria and diversity were studied in different agar plate media during 6 weeks. Agar plate counting was of the same order of magnitude of direct microscopy counts. The highest efficiency of cultivation was 45% attained in marine agar medium. Molecular analysis revealed 10 different phyla of which only four were isolated by the culture dependent method. On the other hand, four taxonomic orders were detected by cultivation but not by the molecular technique. These include bacteria from the phyla Bacillota and Actinomycetota. Our study shows that it is possible to grow more than the traditionally considered 1% of bacteria from a seawater sample using standard agar plate techniques and laboratorial conditions. The results also demonstrate the importance of culture methods to grow bacteria not detected by molecular approaches for future biotechnological applications.

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Section: Introductionmentioning

confidence: 99%

Cultivating marine bacteria under laboratory conditions: Overcoming the “unculturable” dogma

Rodrigues

Carvalho²

2022

Front. Bioeng. Biotechnol.

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“…This compound is utilized in cosmetics as a fragrance, in human medicine as an antimicrobial preservative and local anesthetic, as a solvent for paints, glues, and adhesives, and as a food additive . Benzaldehyde hydrogenation and benzyl chloride hydrolysis are the main processes to produce benzyl alcohol for industrial use, which involve the use of nonrenewable substrates, high temperatures, and byproducts that have a negative impact on the environment . As summarized in Figure , the solid phase consists of a mixture of low-molecular-weight oligomers/polymers with the structure −CH 2 –CH 2 – mixed with boron compounds.…”

Section: Resultsmentioning

confidence: 99%

Cyan Hydrogen Process: A New Route for Simultaneous Hydrogen Production and Carbon Valorization

Di Nardo,

Portarapillo,

Russo

et al. 2024

ACS Omega

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Hydrogen is expected to largely contribute to the near-future circular economy. Today, most hydrogen is still produced from fossil fuels or renewable pathways with low efficiency and high cost. Herein, a proof of concept for a novel hydrogen production process is proposed, here named cyan hydrogen, inspired by a combination of the green and blue processes, due to the key role played by water and the low carbon content in the gas phase, respectively. The developed novel process, recently patented and demonstrated at the lab scale, is based on successive steps in which ethanol (5.0 mL) and water (10.0 mL) are alternately fed, with a fixed initial amount of sodium metaborate (2.0 g), in a batch reactor at 300 °C. Preliminary results showed the simultaneous production of a 95% v/v hydrogen stream, a polymeric byproduct with a repetitive carbon pattern −CH2–CH2–, and a liquid phase rich in oxygenated chemicals at temperatures lower than conventional hydrogen production processes.

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“…We studied in our laboratory the production of benzyl alcohol by Glutamicibacter arilaitensis 232 isolated from a marine sample [ 141 ]. The biocatalyst was identified during screening using ketones and aldehydes as substrates.…”

Section: Bioprocess Developmentmentioning

confidence: 99%

Marine Bioprospecting, Biocatalysis and Process Development

Rodrigues

Carvalho

2022

Microorganisms

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Oceans possess tremendous diversity in microbial life. The enzymatic machinery that marine bacteria present is the result of extensive evolution to assist cell survival under the harsh and continuously changing conditions found in the marine environment. Several bacterial cells and enzymes are already used at an industrial scale, but novel biocatalysts are still needed for sustainable industrial applications, with benefits for both public health and the environment. Metagenomic techniques have enabled the discovery of novel biocatalysts, biosynthetic pathways, and microbial identification without their cultivation. However, a key stage for application of novel biocatalysts is the need for rapid evaluation of the feasibility of the bioprocess. Cultivation of not-yet-cultured bacteria is challenging and requires new methodologies to enable growth of the bacteria present in collected environmental samples, but, once a bacterium is isolated, its enzyme activities are easily measured. High-throughput screening techniques have also been used successfully, and innovative in vitro screening platforms to rapidly identify relevant enzymatic activities continue to improve. Small-scale approaches and process integration could improve the study and development of new bioprocesses to produce commercially interesting products. In this work, the latest studies related to i. the growth of marine bacteria under laboratorial conditions, ii. screening techniques for bioprospecting, and iii. bioprocess development using microreactors and miniaturized systems are reviewed and discussed.

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Process Development for Benzyl Alcohol Production by Whole-Cell Biocatalysis in Stirred and Packed Bed Reactors

Cited by 7 publications

References 77 publications

Cultivating marine bacteria under laboratory conditions: Overcoming the “unculturable” dogma

Cultivating marine bacteria under laboratory conditions: Overcoming the “unculturable” dogma

Cyan Hydrogen Process: A New Route for Simultaneous Hydrogen Production and Carbon Valorization

Marine Bioprospecting, Biocatalysis and Process Development

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