Process development to obtain a cocktail containing cell-wall degrading enzymes with insecticidal activity from Beauveria bassiana

Alves, Eliana A.; Schmaltz, Silvana; Tres, Marcus V.; Zabot, Giovani L.; Kuhn, Raquel C.; Mazutti, Márcio A.

doi:10.1016/j.bej.2019.107484

Cited by 9 publications

(3 citation statements)

References 39 publications

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“…On the other hand, Abd-ElAzeem et al (2019) demonstrated that Acremonium sp and Paecilomyces variotii have the capability to produce protease enzymes which act as cuticle degrading enzyme, so it can play an important role in the control of E. insulana. Finally, Alvesa et al (2020) found that cocktail of cuticle degrading enzymes caused higher larval mortality than each enzyme alone against Phereoeca uterella…”

Section: Fig 4 Screening Of B Bassiana Filtrates Against P Gossypmentioning

confidence: 98%

Biological Control of Pectinophora Gossypiella (Saunders) Using Spores and Supernatants of some Entomopathogenic Fungi

Abd-ElAzeem¹,

El-Sayed

El-Gendy

2020

Journal of Plant Protection and Pathology

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The spores and supernatants of some fungal isolates; Paecilomyces violacea, Trichoderma harzianum and Beauveria bassiana; were evaluated to control the pink bollworm, Pectinophora gossypiella biologically at Plant Protection Research Institute, Sharkia branch, (ARC). All fungi were isolated from soil and dead pink bollworm larvae. B. bassiana was the most active isolate caused the greatest larval and pupal mortality, also the highest deformed adult, also, the lowest numbers of eggs and hatchability percentage. Protease and chitinase produced from B. bassiana. strain had high effects on percent of larval mortality, comparing with control. Identification of Beauvaria sp. was assured by using 18s rRNA with accession number MT644472.1.

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Section: Fig 4 Screening Of B Bassiana Filtrates Against P Gossypmentioning

confidence: 98%

Biological Control of Pectinophora Gossypiella (Saunders) Using Spores and Supernatants of some Entomopathogenic Fungi

Abd-ElAzeem¹,

El-Sayed

El-Gendy

2020

Journal of Plant Protection and Pathology

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“…The coproduction of several enzymes that act as virulence factors against insects in the same fermentation system would reduce the production cost of enzyme-based biopesticides and would help to drive the adoption of them following the current trends [ 6 , 45 ]. Thus, this study looked for strains able to coproduce lytic enzymes, and found that T. harzianum Th180 was able to coproduce chitinase, lipase, and proteases; and M. robertsii Mt015 co-produced proteases and chitinases.…”

Section: Discussionmentioning

confidence: 99%

“…There are several works based on the search for enzymatic co-production under different conditions. For example, optimization of solid fermentation conditions of B. bassiana showed increased production of exocellulase (10%), endocellulase (63%), chitinase (60%), and β -1,3-glucanase (61%) [ 45 ] . Also, B. bassiana in an ultrasound-assisted SmF fermentation produced chitinase and β -1,3 glucanases using a chemically defined culture medium supplemented with broths from agro-industrial residues such as rice and soy bran [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Assessment of Fungal Lytic Enzymatic Extracts Produced Under Submerged Fermentation as Enhancers of Entomopathogens’ Biological Activity

Mejía,

Bautista,

García

et al. 2024

Curr Microbiol

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The application of enzymes in agricultural fields has been little explored. One potential application of fungal lytic enzymes (chitinases, lipases, and proteases) is as an additive to current biopesticides to increase their efficacy and reduce the time of mortality. For this, a screening of lytic overproducer fungi under submerged fermentation with a chemical-defined medium was performed. Then, the enzymatic crude extract (ECE) was concentrated and partially characterized. This characterization consisted of measuring the enzymatic activity (lipase, protease and, chitinase) and determining the enzyme stability after storage at temperatures of − 80, − 20 and, 4 °C. And lastly, the application of these concentrated enzymatic crude extracts (C-ECE) as an enhancer of spores-based fungal biopesticide was proven. Beauveria were not as good producers of lytic enzymes as the strains from Trichoderma and Metarhizium. The isolate M. robertsii Mt015 was selected for the co-production of chitinases and proteases; and the isolate T. harzianum Th180 for co-production of chitinases, lipases, and proteases. The C-ECE of Mt015 had a protease activity of 18.6 ± 1.1 U ml−1, chitinase activity of 0.28 ± 0.01 U ml−1, and no lipase activity. Meanwhile, the C-ECE of Th180 reached a chitinase activity of 0.75 U ml−1, lipase activity of 0.32 U ml−1, and protease activity of 0.24 U ml−1. Finally, an enhancing effect of the enzymatic extracts of M. robertsii (66.7%) and T. harzianum (43.5%) on the efficacy of B. bassiana Bv064 against Diatraea saccharalis larvae was observed. This work demonstrates the non-species-specific enhancing effect of enzymatic extracts on the insecticidal activity of conidial-based biopesticides, which constitutes a contribution to the improvement of biological control agents’ performance.

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Biochemistry of Entomopathogens and Mycoparasites: Metarhizium

Mamani,

Ferreira,

Figueroa

et al. 2024

Entomopathogenic Fungi

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Process development to obtain a cocktail containing cell-wall degrading enzymes with insecticidal activity from Beauveria bassiana

Cited by 9 publications

References 39 publications

Biological Control of Pectinophora Gossypiella (Saunders) Using Spores and Supernatants of some Entomopathogenic Fungi

Biological Control of Pectinophora Gossypiella (Saunders) Using Spores and Supernatants of some Entomopathogenic Fungi

Assessment of Fungal Lytic Enzymatic Extracts Produced Under Submerged Fermentation as Enhancers of Entomopathogens’ Biological Activity

Biochemistry of Entomopathogens and Mycoparasites: Metarhizium

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