Process Parameters Optimization of Gallic Acid Removal from Water by MIEX Resin Based on Response Surface Methodology

Zhou, Qiang; Ding, Lei; Zhu, Yunhua; Ming, Zhong; Yang, Chuchu

doi:10.3390/pr8030273

Cited by 15 publications

(10 citation statements)

References 49 publications

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“…P -value was also used to evaluate the significance of each of the tested variables. The smaller the P-value and larger the sum of squares, the more significant the corresponding factor is [ 49 ]. Results revealed that the factors A (pH) and B (temperature) had a significant effect on paromomycin concentration ( P < 0.0001), temperature being more significant.…”

Section: Discussionmentioning

confidence: 99%

“…A significant interaction between 2 factors means the effect of one factor is dependent on the level of the other [ 52 ]. Contour plots can reveal the significance of the interaction between two factors: an elliptical contour implies a significant interaction between the two factors, whereas a circular contour implies that the interaction between the two factors is weak [ 49 ]. As depicted in the results, the contour plot obtained for AB was oval in shape indicating significant interaction between these variables.…”

Section: Discussionmentioning

confidence: 99%

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Production and statistical optimization of Paromomycin by Streptomyces rimosus NRRL 2455 in solid state fermentation

et al. 2021

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Background Paromomycin is a 2-deoxystreptamine aminocyclitol aminoglycoside antibiotic with broad spectrum activity against Gram-negative, Gram-positive bacteria and many protozoa. This study introduces a strategy for paromomycin production through solid-state fermentation using Streptomyces rimosus subsp. paromomycinus NRRL 2455. Solid state fermentation has gained enormous attention in the development of several products because of their numerous advantages over submerged liquid fermentation. After selecting the best solid substrate, a time course study of paromomycin production was carried out followed by optimization of environmental conditions using response surface methodology. Paromomycin yields obtained using this technique were also compared to those obtained using submerged liquid fermentation. Results Upon screening of 6 different substrates, maximum paromomycin concentration (0.51 mg/g initial dry solids) was obtained with the cost-effective agro-industrial byproduct, corn bran, impregnated with aminoglycoside production media. Optimization of environmental conditions using D-optimal design yielded a 4.3-fold enhancement in paromomycin concentration reaching 2.21 mg/g initial dry solids at a pH of 8.5, inoculum size of 5% v/w and a temperature of 30 °C. Conclusion Compared to submerged liquid fermentation, solid state fermentation resulted in comparable paromomycin concentrations, cost reduction of raw materials, less energy consumption and waste water discharge, which have major implications in industrial fermentation. Therefore, solid state fermentation is a promising alternative to submerged liquid fermentation for paromomycin production. To the best of our knowledge, this is the first report on the optimized paromomycin production through solid state fermentation process.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Production and statistical optimization of Paromomycin by Streptomyces rimosus NRRL 2455 in solid state fermentation

et al. 2021

View full text Add to dashboard Cite

show abstract

“…P value was also used to evaluate the signi cance of each of the tested variables. The smaller the Pvalue and larger the sum of squares, the more signi cant the corresponding factor is [56]. Results revealed that the factors A (pH) and B (temperature) had a signi cant effect on paromomycin concentration (P < 0.0001), temperature being more signi cant.…”

Section: Discussionmentioning

confidence: 95%

“…A signi cant interaction between 2 factors means the effect of one factor is dependent on the level of the other [58]. Contour plots can reveal the signi cance of the interaction between two factors: an elliptical contour implies a signi cant interaction between the two factors, whereas a circular contour implies that the interaction between the two factors is weak [56]. As depicted in the results, the contour plot obtained for AB was oval in shape indicating signi cant interaction between these variables.…”

Section: Discussionmentioning

confidence: 99%

Production and Statistical Optimization of Paromomycin by Streptomyces rimosus NRRL 2455 in Solid State Fermentation

El-Housseiny

Ibrahim

Yassien

et al. 2020

Preprint

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Background: Paromomycin is a 2 deoxystreptamine aminocyclitol aminoglycoside antibiotic with broad spectrum activity against Gram-negative, Gram-positive bacteria and many protozoa. This study introduces a strategy for paromomycin production through solid-state fermentation using Streptomyces rimosus subsp. paromomycinus NRRL 2455. Solid state fermentation has gained enormous attention in the development of several products because of their numerous advantages over submerged liquid fermentation. After selecting the best solid substrate, a time course study of paromomycin production was carried out followed by optimization of environmental conditions using response surface methodology. Paromomycin yields obtained using this technique were also compared to those obtained using submerged liquid fermentation.Results: Upon screening of 6 different substrates, maximum paromomycin concentration (0.51 mg/g initial dry solids) was obtained with the cost-effective agro-industrial byproduct, corn bran, impregnated with aminoglycoside production media. This value was higher than that obtained using submerged liquid fermentation using the same conditions. Optimization of environmental conditions using D optimal design yielded a 4.3-fold enhancement in paromomycin concentration reaching 2.21 mg/g initial dry solids at a pH of 8.5, inoculum size of 5% v/w and a temperature of 30 °C. Conclusion: Compared to submerged liquid fermentation, solid state fermentation resulted in higher paromomycin concentrations, cost reduction of raw materials, less energy consumption and waste water discharge, which have major implications in industrial fermentation. Therefore, SSF is a superior alternative to SLF for paromomycin production. To the best of our knowledge, this is the first report on the optimized paromomycin production through solid state fermentation process.

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“…The smaller the P -value and larger the sum of squares, the more significant the corresponding factor is. 36 , 37 As shown in Table 3 , starch (A) was the most significant factor affecting antifungal production, followed by ammonium chloride (B), pH (C), temperature (D) and finally agitation (E). Temperature and pH are vital physiological parameters influencing the metabolic pathways, hence the generation of various metabolites.…”

Section: Discussionmentioning

confidence: 99%

<p>Optimized Production of the Allylamine Antifungal “Terbinafine” by <em>Lysinibacillus</em> Isolate MK212927 Using Response Surface Methodology</p>

El-Sayed

El-Housseiny

Abdelaziz

et al. 2020

IDR

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Purpose: We aimed to optimize the factors affecting the production of the allylamine antifungal, terbinafine, by Lysinibacillus isolate MK212927, a natural producer of this broadspectrum fungicidal compound. Methods: We employed a central composite design to optimize the five most important variables influencing the production of terbinafine which were carbon source, nitrogen source, temperature, pH and agitation. Results: The optimum conditions were found to be starch 5 g/L, ammonium chloride 5 g/L, temperature 32°C, agitation 150 rpm and pH 7. The actual response (inhibition zone diameter) was highly comparable to the value predicted by the model, indicating a valid model. Using the standard calibration curve of terbinafine, the optimized conditions resulted in an increase in the antifungal metabolite production (terbinafine) by about 1.6-fold (1814.662 µg/mL compared to 1165.550 µg/mL under standardized conditions). Conclusion: This is the first report, to the best of our knowledge, on optimized production of terbinafine by Lysinibacillus species. Hence, these findings may be useful as baseline data for scaling up the production of terbinafine from a natural microbial source.

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Process Parameters Optimization of Gallic Acid Removal from Water by MIEX Resin Based on Response Surface Methodology

Cited by 15 publications

References 49 publications

Production and statistical optimization of Paromomycin by Streptomyces rimosus NRRL 2455 in solid state fermentation

Production and statistical optimization of Paromomycin by Streptomyces rimosus NRRL 2455 in solid state fermentation

Production and Statistical Optimization of Paromomycin by Streptomyces rimosus NRRL 2455 in Solid State Fermentation

<p>Optimized Production of the Allylamine Antifungal “Terbinafine” by <em>Lysinibacillus</em> Isolate MK212927 Using Response Surface Methodology</p>

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