Processing and cryopreservation of human ureter tissues for single-cell and spatial transcriptomics assays

Protocol for optimized dissociation of human scalp tissue for hair follicle transcriptomics by scRNA-seq

“…It should always be kept in mind that there could be bias towards the cell types that are easier to be enzymatically digested when using enzymatic digestion for tissue dissociation. 1 This is very hard to be avoided because of the complex cells’ positions and matrix surrounding them 2 , i.e., the heterogeneous structure and composition of the origin tissue. Therefore, it must be cautious to make conclusions related to proportionality changes of certain cell types between different samples or proportional differences between different cellular compartments.…”