Processing of Lung Lavage Fluid Causes Variability in Bronchoalveolar Cell Count

Mordelet-Dambrine, M; A, Arnoux; Stanislas-Leguern, Gisèle; Sandron, D; Chrétien, J; Huchon, G.

doi:10.1164/arrd.1984.130.2.305

Cited by 54 publications

(28 citation statements)

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“…Cell suspensions (90 l) were stained with 10 l of 0.2% crystal violet and the total cell number was determined microscopically using a Neubauer chamber. The differential cell counts were carried out by cytocentrifuge preparations (Cytospin, Fanem, Brazil) stained with May-Grünwald-Giemsa solution (Mordelet-Dambrine et al, 1984).…”

Section: Quantification Of Cells In Bronchoalveolar Lavage (Bal)mentioning

confidence: 99%

“…Blood aliquots were diluted (1:20) in Türk's fluid (3% acetic acid) for total white cell countings in Neubauer chamber, whereas the differential (qualitative) leukocyte countings were performed in blood smears stained according to Mordelet-Dambrine et al (1984). The recovered lavage of bone marrow (5 ml) was centrifuged (170 × g for 10 min at 20 • C).…”

Section: Blood Leukocytes and Bone Marrow Cell Countsmentioning

confidence: 99%

See 1 more Smart Citation

Reduced allergic lung inflammation in rats following formaldehyde exposure: Long-term effects on multiple effector systems

Lino-dos-Santos-Franco¹,

Domingos²,

Damazo³

et al. 2009

Toxicology

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Section: Quantification Of Cells In Bronchoalveolar Lavage (Bal)mentioning

confidence: 99%

Section: Blood Leukocytes and Bone Marrow Cell Countsmentioning

confidence: 99%

Reduced allergic lung inflammation in rats following formaldehyde exposure: Long-term effects on multiple effector systems

Lino-dos-Santos-Franco¹,

Domingos²,

Damazo³

et al. 2009

Toxicology

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“…[2][3][4] Unfortunately, certain technical aspects of the BAL procedure vary, and there is no standardized protocol for BAL in dogs. [3][4][5][6][7] These technical aspects, such as aliquot volume used and the negative pressure applied for aspiration, affect the qual-ABBREVIATIONS BAL Bronchoalveolar lavage BALF Bronchoalveolar lavage fluid IQR Interquartile range MA Manual aspiration SPA Suction pump aspiration TNCC Total nucleated cell count ity of BALF from healthy dogs. 6,7 To our knowledge, the method of BALF retrieval from dogs has not been evaluated.…”

mentioning

confidence: 99%

“…[3][4][5][6][7] These technical aspects, such as aliquot volume used and the negative pressure applied for aspiration, affect the qual-ABBREVIATIONS BAL Bronchoalveolar lavage BALF Bronchoalveolar lavage fluid IQR Interquartile range MA Manual aspiration SPA Suction pump aspiration TNCC Total nucleated cell count ity of BALF from healthy dogs. 6,7 To our knowledge, the method of BALF retrieval from dogs has not been evaluated. It has been hypothesized that SPA may result in decreased cell viability in BALF because excessive negative pressure can cause cell lysis.…”

mentioning

confidence: 99%

Comparison of bronchoalveolar lavage fluid obtained by manual aspiration with a handheld syringe with that obtained by automated suction pump aspiration from healthy dogs

Woods¹,

Defarges²,

Abrams‐Ogg³

et al. 2014

ajvr

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“…Cytocentrifugation speeds range from 10-51Xg with the most common condition being 22 Xg for 6 min [23]. There is always a risk of losing lymphocytes at lower speeds [27,28].…”

Section: Sputum Processing and Analysismentioning

confidence: 99%

Induced Sputum in Asthma: From Bench to Bedside

Bakakos

Schleich

Alchanatis³

et al. 2011

CMC

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During recent years there has been a growing interest in using non-invasive biomarkers to understand and monitor the airway inflammation in subjects with respiratory tract disorders and mainly asthma and chronic obstructive pulmonary disease (COPD). Sputum induction is generally a well-tolerated and safe procedure and a European Respiratory Society Task Force has published a comprehensive review on sputum methodology. Induced sputum cell count and, to a lesser extent, mediator measurements have been particularly well validated. In asthma, the sputum and the cell culture supernatant can be used for the measurement of a variety of soluble mediators, including eosinophil-derived proteins, nitric oxide (NO) derivatives, cytokines and remodelling-associated proteins. Sputum eosinophilia (> 3%) is a classic feature of asthma although half of the patients seems to be non eosinophilic. Measuring the percentage of sputum eosinophils has proved to be useful in the clinical arena in helping to predict short term response to inhaled corticosteroids (ICS) and tailor the dose of ICS in the severe patients but there is scope for the application of other induced sputum markers potentially useful in clinical practice. The widespread application of induced sputum in asthma across the spectrum of disease severity has given insight into the relationship between airway function and airway inflammation, proposed new disease phenotypes and defined which of these phenotypes respond to current therapy, and perhaps most importantly provided an additional tool to guide the clinical management of asthmatic patients. To date sputum induction is the only non-invasive measure of airway inflammation that has a clearly proven role in asthma management.

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Processing of Lung Lavage Fluid Causes Variability in Bronchoalveolar Cell Count

Cited by 54 publications

References 0 publications

Reduced allergic lung inflammation in rats following formaldehyde exposure: Long-term effects on multiple effector systems

Reduced allergic lung inflammation in rats following formaldehyde exposure: Long-term effects on multiple effector systems

Comparison of bronchoalveolar lavage fluid obtained by manual aspiration with a handheld syringe with that obtained by automated suction pump aspiration from healthy dogs

Induced Sputum in Asthma: From Bench to Bedside

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