Processing of the minor capsid protein of the cauliflower mosaic virus requires a cysteine proteinase

Guidasci, Thierry; Mougeot, Jean-Luc; Lebeurier, G.; Mesnard, Jean-Michel

doi:10.1016/s0923-2516(06)80124-x

Cited by 7 publications

(5 citation statements)

References 34 publications

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“…P3 (15 kDa) is found closely associated with purified CaMV particles (Dautel et al ., 1994). An 11 kDa product resulting from the cleavage of P3 at its C‐terminus by a cysteine proteinase (Guidasci et al ., 1992) is frequently detected when extracts from CaMV infected plants are incubated. Up to now, the biological significance of this cleavage has remained a mystery.…”

Section: Functions Of Camv Proteinsmentioning

confidence: 99%

Cauliflower mosaic virus: still in the news

Haas

Bureau

Geldreich

et al. 2002

Molecular Plant Pathology

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SUMMARY Taxonomic relationship: Cauliflower mosaic virus (CaMV) is the type member of the Caulimovirus genus in the Caulimoviridae family, which comprises five other genera. CaMV replicates its DNA genome by reverse transcription of a pregenomic RNA and thus belongs to the pararetrovirus supergroup, which includes the Hepadnaviridae family infecting vertebrates. Physical properties: Virions are non-enveloped isometric particles, 53 nm in diameter (Fig. 1). They are constituted by 420 capsid protein subunits organized following T= 7 icosahedral symmetry (Cheng, R.H., Olson, N.H. and Baker, T.S. (1992) Cauliflower mosaic virus: a 420 subunit (T= 7), multilayer structure. Virology, 16, 655-668). The genome consists of a double-stranded circular DNA of approximately 8000 bp that is embedded in the inner surface of the capsid. Viral proteins: The CaMV genome encodes six proteins, a cell-to-cell movement protein (P1), two aphid transmission factors (P2 and P3), the precursor of the capsid proteins (P4), a polyprotein precursor of proteinase, reverse transcriptase and ribonuclease H (P5) and an inclusion body protein/translation transactivator (P6). Hosts: The host range of CaMV is limited to plants of the Cruciferae family, i.e. Brassicae species and Arabidopsis thaliana, but some viral strains can also infect solanaceous plants. In nature, CaMV is transmitted by aphids in a non-circulative manner.

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Section: Functions Of Camv Proteinsmentioning

confidence: 99%

Cauliflower mosaic virus: still in the news

Haas

Bureau

Geldreich

et al. 2002

Molecular Plant Pathology

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“…The P5 N-terminal module is an aspartic protease ( Torruella et al, 1989 ) that cleaves itself from the rest of the full-length protein in an autocatalytic manner. P5 is not the only target for P5 protease as it can cleave P7 and process P4 (described above) ( Torruella et al, 1989 ; Guidasci et al, 1992 ).…”

Section: Functions Of Edibs As Virion Factoriesmentioning

confidence: 99%

Setting Up Shop: The Formation and Function of the Viral Factories of Cauliflower mosaic virus

Schoelz

Leisner

2017

Front. Plant Sci.

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Similar to cells, viruses often compartmentalize specific functions such as genome replication or particle assembly. Viral compartments may contain host organelle membranes or they may be mainly composed of viral proteins. These compartments are often termed: inclusion bodies (IBs), viroplasms or viral factories. The same virus may form more than one type of IB, each with different functions, as illustrated by the plant pararetrovirus, Cauliflower mosaic virus (CaMV). CaMV forms two distinct types of IBs in infected plant cells, those composed mainly of the viral proteins P2 (which are responsible for transmission of CaMV by insect vectors) and P6 (required for viral intra-and inter-cellular infection), respectively. P6 IBs are the major focus of this review. Much of our understanding of the formation and function of P6 IBs comes from the analyses of their major protein component, P6. Over time, the interactions and functions of P6 have been gradually elucidated. Coupled with new technologies, such as fluorescence microscopy with fluorophore-tagged viral proteins, these data complement earlier work and provide a clearer picture of P6 IB formation. As the activities and interactions of the viral proteins have gradually been determined, the functions of P6 IBs have become clearer. This review integrates the current state of knowledge on the formation and function of P6 IBs to produce a coherent model for the activities mediated by these sophisticated virus-manufacturing machines.

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“…Cleavage at this second site is not required for reverse transcriptase activity but it is needed for RNase H activity. It has been suggested that a virion-associated cysteine proteinase is involved in the processing of the CaMV ORF3, a minor component of the virus particles (Guidasci et al, 1992;Dautel et al, 1994). Immunoelectron microscopy experiments have shown the presence of the RTBV proteinase in the surface of virus particles; however, it is not known if it is present as a free protein or as part of a larger precursor (Hay et al, 1994).…”

Section: Plant Pararetrovirusesmentioning

confidence: 99%

Proteinases Involved in Plant Virus Genome Expression

GARCIA¹,

FERNANDEZFERNANDEZ²,

LOPEZMOYA³

1999

Proteases of Infectious Agents

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Plant Virus Proteinases 235product but also regulate its activity or subcellular localization by removal of functional domains.Genes of alphalike, carmolike, and sobemolike viruses are expressed through different combinations of strategies acting at the level of transcription (synthesis of subgenomic (sg) RNAs) and/or translation (alternative translation initiation sites, frameshifting, and readthrough at suppressible termination codons). In some cases, gene expression is completed posttranslationally by proteolysis mediated by a virus-encoded proteinase.Proteolytic processing of protein precursors has also been observed in plant pararetroviruses. These viruses have double-stranded (ds) DNA genomes that replicate via RNA intermediates (Hohn and FOtterer, 1997). In this case, the virus-encoded proteinase belongs to the aspartic family and a host cysteine proteinase seems to be involved in the maturation of a viral protein.Here, we have attempted to give an overall view of the use of proteolytic processing by different plant virus groups for the expression of their genomes.

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Processing of the minor capsid protein of the cauliflower mosaic virus requires a cysteine proteinase

Cited by 7 publications

References 34 publications

Cauliflower mosaic virus: still in the news

Cauliflower mosaic virus: still in the news

Setting Up Shop: The Formation and Function of the Viral Factories of Cauliflower mosaic virus

Proteinases Involved in Plant Virus Genome Expression

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