Production and characterization of a bioemulsifier from Yarrowia lipolytica

Amaral, Priscilla Filomena Fonseca; Silva, J.M. da; Lehocký, Marián; Barros‐Timmons, Ana; Coelho, Maria Alice Zarur; Marrucho, Isabel M.; Coutinho, João A. P.

doi:10.1016/j.procbio.2006.03.029

Cited by 171 publications

(111 citation statements)

References 22 publications

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“…1 show that the Emulsification Activity (EA) ranged between 0.1-0.34, with the maximum EA (0.34) was recorded by Geobacillus thermoleovorans Ir1 (JQ912239). The measurement of EA is an indicator for the activity of biosurfactant production (Jazeh et al, 2012 The emulsification activity of Yansan bioemulsifier of Y. lipolytica was 2.63 when using toulen as a carbon source (Amaral et al, 2006). On the other hand, Camargo de Morais et al (2006) noticed that the EA of bioemulsifier produced by P. citrinum was 0.20 when using olive oil.…”

Section: Resultsmentioning

confidence: 99%

“…Biotechnol., 7 (3): 96-107, 2015Singh et al (2011 analyzed exopolysaccharide produced by B. licheniformis using H NMR spectrum, they noticed seventeen anomeric signals, depicting complex and heterogeneous nature. While Amaral et al (2006) indicated that the H NMR signals for bioemulsifier produce by Y. lipolytica attributed to polysaccharide at 3.2-4.4 ppm and the rest of the spectrum referred the presence of low levels of protein. Gudina et al (2015) used NMR to detected the bioemulsifier produced by Paenibacillus sp.…”

Section: Purification Of Biosurfactantmentioning

confidence: 99%

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Characterization of Biosurfactant Produced by a Novel Thermophillic Strain (Geobacillus thermoleovorans JQ 912239)

Al-Jailawi¹,

Nas²,

Azi³

2015

Asian J. of Biotechnology

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Ten thermophillic bacterial isolates were screened for their ability to produce biosurfactant depending on the emulsification activity, emulsification index and surface tension. The results showed that Geobacillus thermoleovorans (JQ 912239) was the most efficient one for biosurfactant production. The chemical composition of partial purified biosurfactant revealed that it consists of 37.7% lipids, 26.2% carbohydrate and 10.7% protein. The partial and/or purified biosurfactant was subjected to Fourier Transform Infrared Spectroscopy (FTIR), High-Performance Liquid Chromatography (HPLC), Gas Chromatography (GC) and Nuclear Magnetic Resonance (NMR) to complete the chemical characterization. The results of the FTIR confirmed that the biosurfactant contains the lipid, carbohydrate and protein. The HPLC analysis revealed that the lipid fraction consists of palmitic acid, stearic acid and oleic acid, the carbohydrate part consists of xylose, mannose and maltose and the protein part consists of three amino acids (aspartic acid, glutamic acid and glutamine). The lipid fraction was further analyzed using GC and NMR. GC analysis indicated that it contains palmitic acid methyl ester (c16:0), stearic acid (c18:0) and oleic acid (c18:1n9c) however, purified biosurfactant contains a high percentage of palmitic acid methyl ester. The NMR analysis for purified biosurfactant detected that the main component of lipid fraction is triglycerides.

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Section: Resultsmentioning

confidence: 99%

Section: Purification Of Biosurfactantmentioning

confidence: 99%

Characterization of Biosurfactant Produced by a Novel Thermophillic Strain (Geobacillus thermoleovorans JQ 912239)

Al-Jailawi¹,

Nas²,

Azi³

2015

Asian J. of Biotechnology

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“…The emulsifying potential of surface active compounds from different yeasts showing no reduction in surface tension, has been evaluated using the emulsification index test (Amaral et al, 2006;Monteiro et al, 2010). Toledo et al (2008) reported the growth and production of exopolymers by three bacteria, Bacillus subtilis 28, Alcaligenes faecalis 212, and Enterobacter sp.…”

Section: The Physiological Roles Of Biosurfactants and Bioemulsifers mentioning

confidence: 99%

Microbiotechnology Based Surfactants and Their Applications

Rahman¹

2016

Frontiers Research Topics

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PublisherFrontiers in Microbiology About FrontiersFrontiers is more than just an open-access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers Journal SeriesThe Frontiers Journal Series is a multi-tier and interdisciplinary set of open-access, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers Journal Series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to QualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation. What are Frontiers Research Topics?Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: researchtopics@frontiersin.org 2 February 2016 | Microbiotechnology Based Surfactants and Their Applications Frontiers in MicrobiologyBiosurfactants are structurally diverse group of bioactive molecules produced by a variety of microorganisms. They are secondary metabolites that accumulate at interfaces, reduce surface tension and form micellar aggregates. This research topic describes few novel microbial strains with a focus on increasing our understanding of genetics, physiology, regulation of biosurfactant production and their commercial potentials. A major stumbling block in the commercialization of b...

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“…It presented high emulsification activity and stability in the pH range of 3.0 -9.0 and was capable of stabilizing oilin-water emulsions with several aliphatic and aromatic hydrocarbons (Amaral et al, 2006).…”

Section: Culture Medium Investigation For Bioemulsifier/ Biosurfactanmentioning

confidence: 99%

Production of bioemulsifiers by Yarrowia lipolytica in sea water using diesel oil as the carbon source

Souza

Salgueiro

Albuquerque

2012

Braz. J. Chem. Eng.

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-The objective of this work was to investigate, on a flask scale, the production of bioemulsifiers by Yarrowia lipolytica in the presence of sea water, supplemented with nitrogen and phosphate sources, using diesel oil as substrate. A full 2 4 factorial design was conducted to investigate the effects and interactions of the nutrient concentrations (diesel oil, urea, ammonium sulfate and monobasic potassium phosphate) on the response variables: emulsification activity and surface tension of the cell-free cultures. High emulsification activities (> 5,4 UEA) were determined after 168 h in all the experiments. The interactions among diesel oil, urea and monobasic potassium phosphate favored the emulsification with statistical significance. A correlation between the increase of emulsification activity and the reduction of surface tension was not identified.

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Production and characterization of a bioemulsifier from Yarrowia lipolytica

Cited by 171 publications

References 22 publications

Characterization of Biosurfactant Produced by a Novel Thermophillic Strain (Geobacillus thermoleovorans JQ 912239)

Characterization of Biosurfactant Produced by a Novel Thermophillic Strain (Geobacillus thermoleovorans JQ 912239)

Microbiotechnology Based Surfactants and Their Applications

Production of bioemulsifiers by Yarrowia lipolytica in sea water using diesel oil as the carbon source

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