Production and in vitro evaluation of xylooligosaccharides generated from corn cobs

Value addition of orange fruit wastes in the enzymatic production of xylooligosaccharides

“…With this criteria, the P value was observed to be P = 1.06E-09*** which is lower than P =0.005. Also, F value is higher than P and Fcrit which was also found in earlier investigations (Samanta et al, 2012b(Samanta et al, , 2013Hsiao, 2006;Akpinar et al, 2009) (*< = 0.5, ** <= 0.01 to 0.05 and *** <= 0.001-0.005).…”

“…The flow rate was maintained at 0.5 ml/min. During the investigation the ZORBAX carbohydrate column (Agilent, USA) was used and the column temperature was fixed at 25°C (Samanta et al, 2012b). The sample was filtered using 0.2 µm membrane (Minigen USA-MG-25020PVDF) and around 20 ul samples was injected in the column using manual injector.…”

“…The quantification of sample XOS was based upon comparison of average peak areas compared with the standards XOS (X1-X5= (xylose, xylobiose, xylotriose, xylotetrose and xylopentose). The quantification was expressed in ng/ml and converted to mg/ml for further analysis (Samanta et al, 2012b;Akpinar et al, 2007). All standards were provided and analysis was performed at National Institute of Animal Nutrition and Physiology Bangalore.…”

The Potential of Pre-Hydrolysis Liquor from the Dissolving Pulp Process as Recovery Source of Xylooligosaccharide - A Mini-Review

“…According to Bragatto et al (2013), pretreatment peroxide-glacial acetic acid (HAc) followed by enzymatic hydrolysis is a suitable method to achieve high yields of XOs production, reaching approximately 300 g of XOs per kg of pretreated sugarcane bagasse (cellulose 44.79 ± 0.01%, hemicelluloses 32.72 ± 0.79%, and lignin 20.85 ± 0.80%). Jayapal et al (2013) studied the optimization conditions of an extraction process by application of alkali combined with steam for sugarcane bagasse (cellulose 35.67 ± 0.15%, hemicelluloses 23.22 ± 0.05%, and lignin 6.04 ± 0.06%). Then, the extracted xylan was hydrolyzed using the endoxylanase enzyme.…”

“…Production of XOs from corncobs (cellulose 27.71 ± 0.08%, hemicelluloses 38.78 ± 0.15%, and lignin 9.4 ± 0.13%) were introduced using a mild alkali extraction and acid or xylanase hydrolysis processes (Samanta et al 2013). Kiran et al (2013) studied XOs production by enzymatic hydrolysis with two xylanase preparations of xylans by alkaline extraction from various lignocellulosic waste biomasses, namely corncob (xylose content 81.9 g/100 g dry xylan), cotton stalk (xylose content 86.9 g/100 g dry xylan), sunflower stalk (xylose content 85.7 g/100 g dry xylan), rice hull (xylose content 67.0 g/100 g dry xylan), and wheat straw (xylose content 74.2 g/100 g dry xylan).…”