Production, Characterization and Cytotoxic Evaluation of Pyocyanin Pigment Extracted from Pseudomonas aeruginosa Isolated from Industrial Soil Resources

Abstract

Thirty-five isolates belonging to the various genus of bacteria family were isolated from 25 different soil samples from various industrial areas from different districts of Tamil Nadu. One out of them were identified as P.aeruginosa (P1) and screened for pyocyanin production using submerged fermentation. The Pyocyanin production reached µg/ml on modified pseudomonas broth. The identification of strain was confirmed by 16s rRNA, the similarity with other strains available in the database was 98% (FDAARGOS_767). P1 was accessed at gene bank with accession number CP041008.1. Identification of the PhzM gene encoding PhzM enzyme was carried out using gene-specific primer followed by PCR. 190bp size gene was amplified using PCR. PhzM gene was confirmed and similarity with (FDAARGOS_767) strain was 100% in the database. Pyocyanin was extracted by standard chloroform extraction method, purified by HPLC (C17 column) and characterized by UV-Vis absorption spectroscopy, Particle size, zeta potential, FT-IR and LC-MS. The SEM analysis of pyocyanin pigment showed the particle size of microencapsulated pyocyanin. The cytotoxic effect of pyocyanin was investigated using VERO cells and it showed a concentration-response relationship between the concentration and cell viability the cytotoxicity increased gradually with the increase of its concentration.

Characterization, application and statistical optimization approach for enhanced production of pyocyanin pigment by Pseudomonas aeruginosa DN9

“…The appearance of absorption band in the region of 1400-1500 cm −1 is may be due to benzene ring's C=C stretch. The presence of peak at 2399 cm −1 is the indication of C=N stretching and broad hump at 1646 cm −1 represent Alkenyl C=C Strech [27][28][29]. Aziz et al [30] demonstrate FTIR spectra of standard phenazine compound, having peaks and stretches in the region of 3475 cm −1 , 3086 cm −1 , 1627 cm −1 and 1458 cm −1 which are in agreement with our reports.…”

Exploring the Antimicrobial and Anticancer Potential of Pyocyanin Produced by Pseudomonas aeruginosa Strain ONO14782

“…The molecular ngerprint of the material was established through Fourier-transform infrared spectroscopy (FTIR), which revealed the side chains of the phenazine-characterized pyocyanin molecule [31]. The spectra indicated the presence of an O-H bond at 3429 cm − 1 , a C-H aromatic bond at 2367 cm − 1 , a C = N bond at 1631 cm − 1 and a C-O bond at 1250 cm − 1 .…”

“…IR spectra were obtained at a resolution of 4 cm − 1 , spanning the 400-4000 cm − 1 range. To understand the chemical makeup of the bioactive substance, the spectrum was examined [31].…”

“…The molecular ngerprint of the material is created by the molecule absorption and transmission that FTIR provides [31]. The side chains of the phenazine-characterized Pyocyanin molecule were shown in…”