Production, Characterization, and Cytotoxicity Effects of Silver Nanoparticles from Brown Alga (Cystoseira myrica)

Abstract

A green, eco-friendly approach to biosynthesizing silver nanoparticles has been reported for marine macroalga (Cystoseira myrica) extract as a reducing agent. Different pH and temperature impact the green synthesis of silver nanoparticles suggesting that the synthesis depends greatly on pH and temperature. The structure and characters of synthesized nanoparticles were confirmed using HR-TEM, DLS, XRD, and FTIR. Cytotoxicity was indicated using provided cell lines of breast carcinoma cells (MCF-7) and human hepatocellular carcinoma cells (HepG2). Shape of silver nanoparticles at pH 9 and 75°C for 30 min was found to be suitable for the biosynthesis process and the AgNPs exhibited a characteristic absorption peak at 434 nm. High Resolution Electron Microscope Transmission reported polydisperse and spherical shapes ranging from 8 to 15 nm. High attractive and repulsive forces between each nanoparticle were recorded with an average zeta-potential value of approximately −29.3 mV. The X-ray diffraction study revealed the crystalline structure of silver nanoparticles. FTIR has shown the bioreduction of silver ions to silver nanoparticles through biomolecules found in algal extract. Silver nanoparticles have been found to have anticancer activity. The cytotoxicity assay was studied against MCF-7 and HepG2 at various concentrations (100, 50, 25, 12.5, 6.25, 3.125, 1.56, 0.78, 0.39, 0.2, and 0.1 μg/mL). By increasing the concentration of AgNPs from 0.1 to 100 μg/mL, the maximum percentage of viability against MCF-7 and HepG2 cell line decreased from 94.55 ± 7.55 to 19.879 ± 0.503 and from 78.56 ± 11.36 to 25.81 ± 2.66 after time exposure, respectively.

Polycladia myrica-based delivery of selenium nanoparticles in combination with radiotherapy induces potent in vitro antiviral and in vivo anticancer activities against Ehrlich ascites tumor

“…Inflammatory cells also appeared around the central vein. These changes could be due to the degeneration of mitochondrial and cytoplasm disorganization ( Mohamed et al, 2022 ).…”

Assessment of Silver Nanoparticles Derived from Brown Algae Sargassum vulgare: Insight into Antioxidants, Anticancer, Antibacterial and Hepatoprotective Effect

“…The AgNPs, synthesized by shrimp shell-extracted chitin as a reducing and stabilizing agent, possessed significant anticancer activity against HepG2 cells with an IC50 value of 57 ± 1.5 µg/mL; these results were confirmed by flow cytometry, which detected the apoptotic and necrotic cell death of HepG2 [43]. The silver nanoparticles bio-fabricated by Cystoseira myrica brown alga possessed cytotoxic activity against both MCF-7 and HepG2 [44]. The biosynthesized Ag-NPs derived from marine alga Chaetomorpha linum were an effective anticancer agent that could prompt apoptosis in the HCT-116 colon cells [45].…”

Recent Advances in Synergistic Effect of Nanoparticles and Its Biomedical Application

“…Both silver (Ag) and gold (Au) nanoparticles have applications in biology, health, and biochemistry [ 49 , 50 , 51 ]. The antibacterial activity of Ag nanoparticles is well known and used in biomedical and consumer products [ 52 , 53 , 54 , 55 , 56 , 57 ]. The toxicity of Ag nanoparticles, however, is a major concern in the biomedical field [ 58 , 59 ].…”