Production, Detection, Extraction, and Quantification of Polyene Antibiotics

Barreales, Eva G.; Aparicio, Jesús F.

doi:10.1007/978-1-0716-1358-0_19

Cited by 1 publication

(2 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is a specialized pathway for the biosynthesis of benzoic aromatic compounds, which gathers the main building blocks needed to build aromatic amino acids (phenylalanine, tyrosine, and tryptophan) in bacteria. It is functionally related to acetate malonate, which is involved in the construction of the benzene nuclei of polyketides required for the closure (zip-assembly) of aromatic nuclei and for the construction of auxin molecules [37,47,48].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Natural Products Biosynthesis by Streptomyces netropsis IMV Ac-5025 under Exogenous Sterol Action

Loboda,

Biliavska,

Iutynska

et al. 2024

Antibiotics

View full text Add to dashboard Cite

Streptomycetes are known as producers of bioactive substances, particularly antibiotics. Streptomyces netropsis IMV Ac-5025 simultaneously produces different classes of antibiotics, including polyene compounds, phytohormones, and sterols, but the metabolic pathways involved in their biosynthesis are largely understudied. The aim of this work was to explore the biosynthesis of polyene antibiotics, sterols, and phytohormones when the producer is cultivated in a nutrient medium supplemented with exogenous β-sitosterol. Gas chromatography and high-performance liquid chromatography were applied to analyze the spectrum of bioactive compounds. The obtained results demonstrated not only an increase in the accumulation of biomass but also polyene antibiotics, intracellular sterols, auxins, and cytokinins, when cultivating S. netropsis IMV Ac-5025 in a liquid medium with the addition of β-sitosterol. The amount of biomass raised 1.5–2-fold, whilst the sum of polyene antibiotics increased 4.5-fold, sterols’ sum (ergosterol, cholesterol, stigmasterol, β-sitosterol, and 24-epibrassinolide) by 2.9-fold, auxins’ sum (indole-3-acetic acid, indole-3-acetic acid hydrazide, indole-3-carbinol, indole-3-butyric acid, indole-3-carboxaldehyde, and indole-3-carboxylic acid) by 6-fold, and cytokinins’ sum (zeatin, isopentyladenine, zeatin riboside, and isopentenyladenosine) by 11-fold. Thus, we put forward the hypothesis that β-sitosterol plays a regulatory role in the network of biosynthetic reactions of S. netropsis IMV Ac-5025.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The software responded to the height of the peak, which corresponded to candidine or tetraene compounds. The peak area indicated the amount of antibiotic and was supplemented with a control [36,37].…”

Section: Determination Of Polyene Antibiotics By Spectrodensitometric...mentioning

confidence: 99%