Production of 3-hydroxypropionic acid from acetate using metabolically-engineered and glucose-grown Escherichia coli

Lama, Suman; Kim, Yeon-Hee; Nguyen, Duong; Im, Chae Ho; Sankaranarayanan, Mugesh; Park, Sunghoon

doi:10.1016/j.biortech.2020.124362

Cited by 28 publications

(14 citation statements)

References 23 publications

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“…To sustain normal growth of cells as well as improve 3‐HP yields, two‐stage strategy was harnessed whereby cell growth was relied on glucose and 3‐HP production was come from acetate. In this way, the yield of 3‐HP was up to 7.3 g/L (Lama et al, 2021). In our recent work, malonic acid was utilized as a carbon source to produce 3‐HP in E. coli when the genes mcr (encoding MCR) from C. aurantiacus and matB (encoding malonyl‐CoA synthetase, which is capable of generating malonyl‐CoA directly from malonic acid) from Rhodopseudomonas palustris were introduced (B. Liang et al, 2019).…”

Section: Producing 3‐hp From Other Carbon Sourcesmentioning

confidence: 99%

Recent advances, challenges and metabolic engineering strategies in the biosynthesis of 3‐hydroxypropionic acid

Liang

Sun

Nie

et al. 2022

Biotech & Bioengineering

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As an attractive and valuable platform chemical, 3‐hydroxypropionic acid (3‐HP) can be used to produce a variety of industrially important commodity chemicals and biodegradable polymers. Moreover, the biosynthesis of 3‐HP has drawn much attention in recent years due to its sustainability and environmental friendliness. Here, we focus on recent advances, challenges, and metabolic engineering strategies in the biosynthesis of 3‐HP. While glucose and glycerol are major carbon sources for its production of 3‐HP via microbial fermentation, other carbon sources have also been explored. To increase yield and titer, synthetic biology and metabolic engineering strategies have been explored, including modifying pathway enzymes, eliminating flux blockages due to byproduct synthesis, eliminating toxic byproducts, and optimizing via genome‐scale models. This review also provides insights on future directions for 3‐HP biosynthesis.

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Section: Producing 3‐hp From Other Carbon Sourcesmentioning

confidence: 99%

Recent advances, challenges and metabolic engineering strategies in the biosynthesis of 3‐hydroxypropionic acid

Liang

Sun

Nie

et al. 2022

Biotech & Bioengineering

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“…More recently, Lama et al (2021) developed GS-upregulated E. coli (∆poxB∆adhE∆ldhA∆iclR mcr ++ ) that produced 6.49 g/L 3-HP with yield of 0.39 g/g acetate and productivity of 0.15 g/L/h in two-stage cultivation mode. The TRY are the highest ever in 3-HP production from acetate.…”

Section: -Hydroxypropionic Acid (3-hp)mentioning

confidence: 99%

Acetate as a potential feedstock for the production of value-added chemicals: Metabolism and applications

Kim

Lama

Agrawal

et al. 2021

Biotechnology Advances

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“…The fumarate level is directly related to the downstream consumption pathway toward malate (Figure 1A). Though the reaction between fumarate and malate is reversible, It was reported that the flow in the malate direction (glyoxylate cycle) is blocked due to the suppressed expression of aceA, aceB, and acek in glyoxylate shunt by iclR, a DNA-binding transcriptional repressor gene, and the glyoxylate shunt can be ignited with the knockdown of iclR (Figure 1A) [28]. Therefore, to weaken the valine biosynthesis pathway, iclR in B0016-02 was knocked out, generating the B0016-03 strain.…”

Section: Regulation In the Tca Modulementioning

confidence: 99%

Enhancement of β-Alanine Biosynthesis in Escherichia coli Based on Multivariate Modular Metabolic Engineering

Zhou

2021

Biology

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β-alanine is widely used as an intermediate in industrial production. However, the low production of microbial cell factories limits its further application. Here, to improve the biosynthesis production of β-alanine in Escherichia coli, multivariate modular metabolic engineering was recruited to manipulate the β-alanine biosynthesis pathway through keeping the balance of metabolic flux among the whole metabolic network. The β-alanine biosynthesis pathway was separated into three modules: the β-alanine biosynthesis module, TCA module, and glycolysis module. Global regulation was performed throughout the entire β-alanine biosynthesis pathway rationally and systematically by optimizing metabolic flux, overcoming metabolic bottlenecks and weakening branch pathways. As a result, metabolic flux was channeled in the direction of β-alanine biosynthesis without huge metabolic burden, and 37.9 g/L β-alanine was generated by engineered Escherichia coli strain B0016-07 in fed-batch fermentation. This study was meaningful to the synthetic biology of β-alanine industrial production.

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Production of 3-hydroxypropionic acid from acetate using metabolically-engineered and glucose-grown Escherichia coli

Cited by 28 publications

References 23 publications

Recent advances, challenges and metabolic engineering strategies in the biosynthesis of 3‐hydroxypropionic acid

Recent advances, challenges and metabolic engineering strategies in the biosynthesis of 3‐hydroxypropionic acid

Acetate as a potential feedstock for the production of value-added chemicals: Metabolism and applications

Enhancement of β-Alanine Biosynthesis in Escherichia coli Based on Multivariate Modular Metabolic Engineering

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