Production of a new tissue-engineered adipose substitute from human adipose-derived stromal cells

Melatonin pretreatment of human adipose tissue-derived mesenchymal stromal cells enhances their prosurvival and protective effects on human kidney cells

“…hASCs were isolated as previously described (40) and kindly provided by Dr. Julie Fradette (Université Laval, LOEX, Quebec City, Quebec). More specifically, as approved by the ethics committee, these ASCs were isolated from subcutaneous fat lipoaspirated for cosmetic purposes from three women aged 38, 40, and 46 (identified as LA38, LA40, and LA46, and in this study as donor 1, 2, and 3, respectively) with a mean body mass index of 22.4 Ϯ 2.8 (SD).…”

Insulin Regulates Fusion of GLUT4 Vesicles Independent of Exo70-mediated Tethering

“…Although the differentiated 3T3-L1 cells never develop into fully mature adipose cells and are maintained in culture only for a relatively short period after differentiation, mature adipose cells can be cultured only for a few days after isolation, which limits the application of techniques such as small interfering RNA. New strategies involving stromal cells derived from adipose tissue have shown a potential to develop into mature adipose cells that could be kept in culture without losing the adipose phenotype (28). This promises to be an invaluable tool for answering the question how differentiation and maturation of adipocytes affects molecular machinery involved in GLUT4 exocytosis.…”

Magnetic Resonance Imaging of Human Tissue-Engineered Adipose Substitutes

“…For comparative purposes, reconstructed connective tissue sheets devoid of adipocytes were also produced by using the standard medium throughout the culture period. 32 Tissue reconstruction proceeded as a multistep protocol based on adaptations of previously published methods from our group. 32,33,35 The adipose substitutes were produced according to two main models based on initial cell seeding into six-well plates for series 1 and 2 (9.6 cm 2 ), while series 3 consisted of a model produced from initially larger culture vessels (57-87 cm 2 ).…”

“…The resulting connective cell sheets can then be superposed to produce thicker connective tissues. 32,33 If an adipogenic induction step is performed in parallel to matrix stimulation, then the resulting tissue sheets feature functional adipocytes filled with lipids. 32,34 These reconstructed tissues do not contain synthetic or exogenous biomaterials, and their production can be customized for shape and thickness.…”

“…They can perform b-adrenergic-mediated lipolysis and secrete various important adipokines. 32 The implanted reconstructed AT substitutes were visualized and followed up in T 1 -w. MRI to enable volume integration. In parallel, the proton relaxation properties of reconstructed AT substitutes were studied, providing useful insights to optimize MRI visualization and revealing the potential of NMR/MRS in the field of reconstructed AT assessment.…”