Production of antimicrobial blue green pigment Pyocyanin by marine Pseudomonas aeruginosa

Abstract

This study focused on isolation and molecular identification of marine bacteria which producing bioactive pigment. For that, 10 pigmented bacteria were isolated from 3 water samples collected from Mediterranean Sea, Alex., Egypt. One isolate was had efficient value to produce blue green pigment. This one was identified using molecular biology techniques and recorded in GeneBank under title of Pseudomonas aeruginosa strain OSh1 with accession number KT032066. The produced pigment was extracted, purified and characterized as pyocyanin. The pathway of pyocyanin production contains many sequential genes, the important one is phzS. This gene was detected genetically in this study using specific primers and gave PCR product with 448 bp. To confirm that, the inducible proteins for pyocyanin synthesis genes were documented using SDS-PAGE. The results represent found 2 proteins with 36 and 43 kDa for phzM and phzS, respectively. The biological activity of the produced pyocyanin at different concentrations was investigated as antimicrobial agent. The antibacterial activity was ranged between 31- 42 mm of inhibition zone. Whereas, the inhibition zone for antifungal activity was ranged between 34- 35 mm. The results indicate the produced pyocyanin had high activity against multi-drug resistant microbes and we can use it as marine antibiotic.

A purified and lyophilized Pseudomonas aeruginosa derived pyocyanin induces promising apoptotic and necrotic activities against MCF-7 human breast adenocarcinoma

“…The concentration of pyocyanin in the three different solvents, stored in the refrigerator, was detected by days as described by [ 26 ]. The intact and degraded, after color change, pyocyanin was subjected to spectroscopic analysis over a range of 200–400 nm using a UV–Vis spectrophotometer (PG, T80 + , England) and compared to standard pyocyanin (Sigma-Aldrich, St Louis, MO, USA) as prescribed by [ 51 ]. Finally, for better estimation of pyocyanin stability, we checked the antibacterial activity against Escherichia coli , obtained from our lab, for the dissolved pyocyanin in SDW, day 1 lyophilized pyocyanin, the same pyocyanin solution after 60 days, after losing its blue color, stored at − 18 °C, and the dissolved pyocyanin in SDW after one year of storage its lyophilized form at − 18 °C as prescribed by [ 8 , 52 ].…”

Isolation of Haematococcus lacustris as source of novel anti-multi-antibiotic resistant microbes agents; fractionation and identification of bioactive compounds

“…One of the biggest issues in healthcare today is treating infectious diseases, particularly those brought on by resistant bacteria. Major bacterial species have developed their resistance system to antibiotics; some of these species are now anticipated to be resistant to most or all antimicrobials, leading to "antibiotic resistance emergency" [3] , [4] . Because of the widespread bacterial resistance to current antibiotics, it is crucial to discover novel bioactive chemicals.…”

Nano-Bioremediation of Municipal Wastewater Using Myco-Synthesized Iron Nanoparticles

“…Because of nanomaterials have high surface/volume ratio, it has been explored to detect and treat pollutants in various environmental matrixes like wastewater, soil and sediment by means of adsorption, oxidation-reduction, surface complication and other mechanisms [1]. Modern technology for pathogens disinfectant means to produce and apply new substances have antimicrobial activity such as plant or microbial agents [10][11][12][13], new chemical materials or derivatives [14,15] and new composites [16][17][18]. Also, many researchers introduced new adsorbents like lignin derivatives and low-cost lignocellulosic substances for wastewater remediation from heavy metals and toxic dyes [19][20].…”