Production of Bio‐Oil from Waste Cooking Oil by Pyrolysis

Feria, J.; Galeano, Gloria Astrid Nausa; Malagón-Romero, Dionisio

doi:10.1002/ceat.202100018

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…5 C, however, indicated otherwise, with 35% lower amyrin titers observed in the engineered Yarrowia cultures with Mg 2+ supplementation. Conservatively, around 190 million metric tons of WCO are generated each year [ 41 ], thus raising concerns of waste management. Fermentative valorization of WCO can be an effective approach to both reduce waste as well as provide sustainable feedstock for the biomanufacturing industry.…”

Section: Resultsmentioning

confidence: 99%

Enhanced production of amyrin in Yarrowia lipolytica using a combinatorial protein and metabolic engineering approach

Kong

Ling

et al. 2022

Microb Cell Fact

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Background Amyrin is an important triterpenoid and precursor to a wide range of cosmetic, pharmaceutical and nutraceutical products. In this study, we metabolically engineered the oleaginous yeast, Yarrowia lipolytica to produce α- and β-amyrin on simple sugar and waste cooking oil. Results We first validated the in vivo enzymatic activity of a multi-functional amyrin synthase (CrMAS) from Catharanthus roseus, by expressing its codon-optimized gene in Y. lipolytica and assayed for amyrins. To increase yield, prevailing genes in the mevalonate pathway, namely HMG1, ERG20, ERG9 and ERG1, were overexpressed singly and in combination to direct flux towards amyrin biosynthesis. By means of a semi-rational protein engineering approach, we augmented the catalytic activity of CrMAS and attained ~ 10-folds higher production level on glucose. When applied together, protein engineering with enhanced precursor supplies resulted in more than 20-folds increase in total amyrins. We also investigated the effects of different fermentation conditions in flask cultures, including temperature, volumetric oxygen mass transfer coefficient and carbon source types. The optimized fermentation condition attained titers of at least 100 mg/L α-amyrin and 20 mg/L β-amyrin. Conclusions The design workflow demonstrated herein is simple and remarkably effective in amplifying triterpenoid biosynthesis in the yeast Y. lipolytica.

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Section: Resultsmentioning

confidence: 99%

Enhanced production of amyrin in Yarrowia lipolytica using a combinatorial protein and metabolic engineering approach

Kong

Ling

et al. 2022

Microb Cell Fact

View full text Add to dashboard Cite

show abstract

“…Therefore, the reclaim and reuse of WCO can effectively address the scarcity of petroleum resources, reduce environmental pollution, and safeguard public health. 1–3 Although there are numerous ways to repurpose WCO and it can produce a variety of products like biofuels (biodiesel, 4–7 bioethanol 8–10 and biogas 11,12 ), biolubricants, 13–15 biosurfactants, 16–18 asphalt modifier, 19–22 washing product, 23,24 etc. , traditional products based on WCO often exhibit quite a low-profit margin (often <20%) and necessitate large-scale production lines and significant investments.…”

Section: Introductionmentioning

confidence: 99%