Production of donor-derived offsprings by allogeneic transplantation of oogonia in the adult Japanese flounder (Paralichthys olivaceus)

Ren, Yuqin; Sun, Zhizhong; Wang, Yufen; Yu, Qin; Wang, Guixing; He, Zhongwei; Liu, Yufeng; Jiang, Xiufeng; Kang, Xianjiang; Hou, Jilun

doi:10.1016/j.aquaculture.2021.736977

Cited by 4 publications

(7 citation statements)

References 38 publications

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“…In loach ( Misgurnus anguillicaudatus ), 30–36% density fraction consisted of about 60% of type A and early-type B spermatogonia [ 71 ]. In olive flounder ( Paralichthys olivaceus ), the proportion of vasa -positive ovarian cells increased from 37.8% to 83.6% after PDGC [ 72 ]. In Siberian sturgeon, cell populations containing vasa -positive testicular cells (79.4% of total) and ovarian cells (70.8% of total) were obtained by PDGC [ 39 ].…”

Section: Factors Affecting the Success Of Germ Cell Transplantationmentioning

confidence: 99%

“…By contrast, high survival rates (greater than 75%) of recipients were observed in adult recipients ( Appendix A , Table A4 ). Even though wild-type adults were treated with high temperature and busulfan for eliminating endogenous germ cells before transplantation, it did not cause significant decreases in the survival of recipients when treated with the optimized conditions [ 72 , 100 ].…”

Section: Factors Affecting the Success Of Germ Cell Transplantationmentioning

confidence: 99%

“…Co-treatment of high temperature and busulfan has been applied to adult recipients for ablating endogenous germ cells ( Appendix A , Table A4 ). High temperature arrests meiosis and induces massive apoptosis of spermatogenic cells in fish [ 32 , 72 , 128 ]. Busulfan is an alkylating agent that produces intrastrand or interstrand crosslinks of DNA, blocking DNA replication, cell proliferation, and differentiation [ 129 ].…”

Section: Factors Affecting the Success Of Germ Cell Transplantationmentioning

confidence: 99%

“…However, with this treatment, endogenous germ cells were not completely ablated, and the remaining GSCs could recover and repopulate gonads [ 128 ], impeding successful germ cell transplantation. In addition, as an inappropriate treatment can cause high mortality and inefficient germ cell ablation [ 72 ], optimizing procedures to achieve successful endogenous germ cell depletion is necessary.…”

Section: Factors Affecting the Success Of Germ Cell Transplantationmentioning

confidence: 99%

“…Even though at least 500 recipients were transplanted with donor germ cells of each species, only limited recipients (2.2 to 6.8%; estimated) carried donor-derived gametes at 2 to 3 years after transplantation due to their low survival during early development. By contrast, when allogeneic transplantation was conducted using adult male olive flounder co-treated with high temperature and busulfan, all recipients survived, and 11.7% of total recipients produced donor-derived functional sperms within 10 months after transplantation [ 72 ]. Because GAT had higher success and survival rates and a shorter generation time than GPT, GAT would be a superb option in these species and other species that encounter the same challenges of low survival rate during early development.…”

Section: Obstacles and Potential Solutions Of Germ Cell Transplantati...mentioning

confidence: 99%

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Advantages, Factors, Obstacles, Potential Solutions, and Recent Advances of Fish Germ Cell Transplantation for Aquaculture—A Practical Review

Ryu

Wong

2022

Animals

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Germ cell transplantation technology enables surrogate offspring production in fish. This technology has been expected to mitigate reproductive barriers, such as long generation time, limited fecundity, and complex broodstock management, enhancing seed production and productivity in aquaculture. Many studies of germ cell transplantation in various fish species have been reported over a few decades. So far, surrogate offspring production has been achieved in many commercial species. In addition, the knowledge of fish germ cell biology and the related technologies that can enhance transplantation efficiency and productivity has been developed. Nevertheless, the commercial application of this technology still seems to lag behind, indicating that the established models are neither beneficial nor cost-effective enough to attract potential commercial users of this technology. Furthermore, there are existing bottlenecks in practical aspects such as impractical shortening of generation time, shortage of donor cells with limited resources, low efficiency, and unsuccessful surrogate offspring production in some fish species. These obstacles need to be overcome through further technology developments. Thus, we thoroughly reviewed the studies on fish germ cell transplantation reported to date, focusing on the practicality, and proposed potential solutions and future perspectives.

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Section: Factors Affecting the Success Of Germ Cell Transplantationmentioning

confidence: 99%

Section: Factors Affecting the Success Of Germ Cell Transplantationmentioning

confidence: 99%

Section: Factors Affecting the Success Of Germ Cell Transplantationmentioning

confidence: 99%

Section: Factors Affecting the Success Of Germ Cell Transplantationmentioning

confidence: 99%

Section: Obstacles and Potential Solutions Of Germ Cell Transplantati...mentioning

confidence: 99%

See 3 more Smart Citations

Advantages, Factors, Obstacles, Potential Solutions, and Recent Advances of Fish Germ Cell Transplantation for Aquaculture—A Practical Review

Ryu

Wong

2022

Animals

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Production of albino chub mackerel (Scomber japonicus) by slc45a2 knockout and the use of a positive phototaxis-based larviculture technique to overcome the lethal albino phenotype

Kawamura

Hasegawa²,

Yamauchi³

et al. 2022

Aquaculture

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Enhancement of zebrafish sperm production via a large body-sized surrogate with germ cell transplantation

et al. 2023

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Zebrafish (Danio rerio) is a commonly-used vertebrate model species for many research areas. However, its low milt volume limits effective cryopreservation of sperm from a single individual and often precludes dividing a single semen sample to conduct multiple downstream procedures such as genomic DNA/RNA extraction and in-vitro fertilization. Here, we apply germ stem cell transplantation to increase zebrafish sperm production in a closely related larger species from the same subfamily, giant danio Devario aequipinnatus. The endogenous germ cell of the host is depleted by dead-end morpholino antisense oligonucleotide. Histology of the sterile gonad and quantitative PCR of gonadal tissue reveals all sterile giant danio develop the male phenotype. Spermatogonial cells of Tg(ddx4:egfp) transgenic zebrafish are transplanted into sterile giant danio larvae, and 22% of recipients (germline chimera) produce donor-derived sperm at sexual maturation. The germline chimera produce approximately three-fold the volume of sperm and 10-fold the spermatozoon concentration of the donor. The donor-derived sperm is functional and gives rise to viable progeny upon fertilization of donor oocytes. We show that the issue of low milt volume can be effectively addressed by employing a larger surrogate parent.

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Production of donor-derived offsprings by allogeneic transplantation of oogonia in the adult Japanese flounder (Paralichthys olivaceus)

Cited by 4 publications

References 38 publications

Advantages, Factors, Obstacles, Potential Solutions, and Recent Advances of Fish Germ Cell Transplantation for Aquaculture—A Practical Review

Advantages, Factors, Obstacles, Potential Solutions, and Recent Advances of Fish Germ Cell Transplantation for Aquaculture—A Practical Review

Production of albino chub mackerel (Scomber japonicus) by slc45a2 knockout and the use of a positive phototaxis-based larviculture technique to overcome the lethal albino phenotype

Enhancement of zebrafish sperm production via a large body-sized surrogate with germ cell transplantation

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