Production of eurycomanone and polysaccharides through adventitious root culture of Eurycoma longifolia in a bioreactor

Fan, Mingzhi; An, Xiaolong; Cui, Xi-Hua; Jiang, Xiaolong; Piao, Xiangfan; Jin, Meizhong; Lian, Mei-Lan

doi:10.1016/j.bej.2021.108013

Cited by 13 publications

(4 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In response to ROS, plants activate the defense mechanism and either release enzymatic or non-enzymatic components (polyphenolics, ascorbate, tocopherol, glutathione, superoxide dismutase, and peroxidases) that neutralize the damaging effect of ROS but increase the release of secondary cell products that are the actual metabolites needed for various medicinal uses [ 71 ]. Similar reports have been published on the development of adventitious roots in medicinally important species of Plumbago zeylanica , Eurycoma longifolia , and Centella asiatica for the production of highly valued secondary metabolites [ 72 , 73 , 74 , 75 , 76 ].…”

Section: Resultssupporting

confidence: 65%

Elicitation of Submerged Adventitious Root Cultures of Stevia rebaudiana with Cuscuta reflexa for Production of Biomass and Secondary Metabolites

Ahmad

Khan

et al. 2021

Molecules

View full text Add to dashboard Cite

Stevia rebaudiana is an important medicinal plant that belongs to the Asteraceae family. The leaves of Stevia rebaudiana are a rich source of many health-promoting agents such as polyphenols, flavonoids, and steviol glycoside, which play a key role in controlling obesity and diabetes. New strategies such as the elicitation of culture media are needed to enhance the productivity of active components. Herein, the Cuscuta reflexa extracts were exploited as elicitors to enhance the productivity of active components. Cuscuta reflexa is one of the parasitic plants that has the ability to elongate very fast and cover the host plant. Consequently, it may be possible that the addition of Cuscuta reflexa extracts to adventitious root cultures (ADR) of Stevia rebaudiana may elongate the root more than control cultures to produce higher quantities of the desired secondary metabolites. Therefore, the main objective of the current study was to investigate the effect of Cuscuta reflexa extract as a biotic elicitor on the biomass accumulation and production of antioxidant secondary metabolite in submerged adventitious root cultures of Stevia rebaudiana. Ten different concentrations of Cuscuta reflexa were added to liquid media containing 0.5 mg/L naphthalene acetic acid (NAA). The growth kinetics of adventitious roots was investigated for a period of 49 days with an interval of 7 days. The maximum biomass accumulation (7.83 g/3 flasks) was observed on medium containing 10 mg/L extract of Cuscuta reflexa on day 49. As the concentration of extract increases in the culture media, the biomass gradually decreases after 49 days of inoculation. In this study, the higher total phenolics content (0.31 mg GAE/g-DW), total flavonoids content (0.22 mg QE/g-DW), and antioxidant activity (85.54%) were observed in 100 mg/L treated cultures. The higher concentration (100 mg/L) of Cuscuta reflexa extract considerably increased the total phenolics content (TPC), total phenolics production (TPP), total flavonoids content (TFC), total flavonoids production (TFP), total polyphenolics content (TPPC), and total polyphenolics production (TPPP). It was concluded that the extract of Cuscuta reflexa moderately improved biomass accumulation but enhanced the synthesis of phenolics, flavonoids, and antioxidant activities. Here, biomass’s independent production of secondary metabolites was observed with the addition of extract. The present study will be helpful to scale up adventitious roots culture into a bioreactor for the production of secondary metabolites rather than biomass accumulation in medicinally important Stevia rebaudiana.

show abstract

Section: Resultssupporting

confidence: 65%

Elicitation of Submerged Adventitious Root Cultures of Stevia rebaudiana with Cuscuta reflexa for Production of Biomass and Secondary Metabolites

Ahmad

Khan

et al. 2021

Molecules

View full text Add to dashboard Cite

show abstract

“…Moreover, recently, Fan et al [140] showed the enhanced production of eurycomanone and polysaccharides in an adventitious root culture of Eurycoma longifolia using the bioreactor system. In Gynura procumbens, the production of root biomass was compared to different liquid cultures (shake flask, balloon-type bubble bioreactor and temporary-immersion bioreactor).…”

Section: Adventitious Root Culture In Bioreactorsmentioning

confidence: 99%

Adventitious Root Culture—An Alternative Strategy for Secondary Metabolite Production: A Review

et al. 2022

View full text Add to dashboard Cite

Medicinal plants are valuable sources of botanical drugs, extracts and pure compounds. Although several species can be propagated or collected, the access to herbal material is limited in certain cases. The protection of natural habitats and biodiversity demands new sources to provide plant secondary metabolites of medical importance. Adventitious root culture is used to harvest the secondary metabolites from the medicinally important plants, thereby offering an alternative to collection and propagation of medicinal plants. In this review, we comprehensively summarize the previously published data on the use of adventitious root cultures for numerous therapeutic plants. Adventitious roots showed elevated growth rates and production of pharmaceutically important metabolites under sterilized condition with optimized plant-growth regulators in culture media. In the present study, major influencing factors, such as the stages involved in the process of adventitious root formation, medium composition and type of growth regulators, specifically the effect of different auxins on the initiation and formation of roots, are discussed. Elicitation strategies using biotic (yeast extracts, chitosan and pectin) and abiotic factors (MJ, SA, CuSO4, AgNO3, NaCl) that affect the in vitro growth of adventitious roots and the role of bioreactors, which are new advancements in the scale-up process, are also highlighted. The development of adventitious root cultures for the production of secondary metabolites of medicinal importance is a perspective that is advantageous from ecological and economical aspects as well.

show abstract

“…The results showed that a ratio of 1:2 NH 4 + and NO 3 − was optimal for growth and synthesis. In Fan et al [ 101 ], ARs were fed to bioreactors containing ¾ MS to optimize the growth conditions. The results showed that 40 g/L sucrose, a 5.0 g/L cell density, and a 0.05 vvm aeration rate were optimum for the growth synthesis of eurycomanone.…”

Section: Techniques For Improving the Synthesis Of Bioactive Compound...mentioning

confidence: 99%

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

Sale,

Subramaniam,

Mad’ Atari

2023

Plants

View full text Add to dashboard Cite

Over the last two decades, there has been a concerted effort by researchers to mass propagate Eurycoma longifolia and improve the yield of its very important and sought-after anti-cancer and aphrodisiac bioactive compounds. To achieve this, various techniques have been used to mass propagate and improve the yield of these bioactive compounds in tissue cultures. These techniques include the optimization of media conditions and application of various types and combinations of plant growth regulators (PGRs). In addition, some elicitation techniques have been used to improve the synthesis of these bioactive compounds. However, in comparison with other herbal species with similar economic importance, many techniques have not been applied to E. longifolia. Adopting the most recent methodologies would ensure efficiency and sustainability in the in vitro production of bioactive compounds in E. longifolia. Therefore, in this review, we present an up-to-date record on the success stories in the tissue culture techniques and synthesis of bioactive compounds. In addition, we attempted to identify some of the missing links on the road to the effective and sustainable biotechnological utilization of this super important biological resource.

show abstract

Production of eurycomanone and polysaccharides through adventitious root culture of Eurycoma longifolia in a bioreactor

Cited by 13 publications

References 29 publications

Elicitation of Submerged Adventitious Root Cultures of Stevia rebaudiana with Cuscuta reflexa for Production of Biomass and Secondary Metabolites

Elicitation of Submerged Adventitious Root Cultures of Stevia rebaudiana with Cuscuta reflexa for Production of Biomass and Secondary Metabolites

Adventitious Root Culture—An Alternative Strategy for Secondary Metabolite Production: A Review

Trends in the Tissue Culture Techniques and the Synthesis of Bioactive Compounds in Eurycoma longifolia Jack—Current Status and Future Perspectives

Contact Info

Product

Resources

About