Production of Polyclonal Antibodies and Development of Fluorescence Polarization Immunoassay for Sulfanilamide

Eremin, Sergei A.; Murtazina, N. R.; Ermolenko, Dmitri N.; Zherdev, Anatoly V.; Мартьянов, А. А.; Yazynina, Elena V; Michura, Irina V.; Formanovsky, A. A.; Dzantiev, B.G.

doi:10.1081/al-200054059

Cited by 37 publications

(17 citation statements)

References 29 publications

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“…MIPs can be prepared using a number of ways, such as chemical grafting, soft lithography technique, molecular self‐assembly approach, electropolymerization etc. 11, 12. Molecular imprinting can also be used for the electrosynthesis of conducting polymers through galvanostatic, potentiostatic and cyclic voltammetric methods 13–15.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of Sulfanilamide Using Pencil Graphite Electrode Based on Molecular Imprinting Technology

Tadi¹,

Motghare²,

Ganesh³

2014

Electroanalysis

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Molecularly imprinted polymer (MIP) for sulfanilamide (SN) sensing is prepared through in‐situ electropolymerization of pyrrole (Py) on pencil graphite electrode (PGE). Computational study using density functional theory with B3LYP level is performed to analyze and evaluate the template‐monomer geometry. Among the various functional monomers studied pyrrole is found to have the highest binding interaction energy with SN and it is chosen as a functional monomer. Electropolymerization of pyrrole in the presence of SN on PGE is carried out using cyclic voltammetry. Structural morphology of the imprinted polymer is characterized by field emission scanning electron microscopy (FESEM) studies. Quantitative measurements of MIP based SN detection are performed by using cyclic voltammetry and differential pulse voltammetry studies. Several important kinetic parameters influencing the performance of SN sensor such as limit of detection, linear concentration range and sensitivity etc. are determined and analyzed. Under the optimized conditions, MIP based SN sensor proposed in this work has a very low detection limit of 2.0×10−8 M (S/N=3) and possesses two linear ranges from 5.0×10−8 to 1.1×10−6 M and 1.1×10−6 to 48×10−6 M with a sensitivity value of 1.158 and 0.012 µA/µM respectively. This particular sensor shows a good selectivity towards SN in presence of potential other structural analogue interferences namely sulfamethaxazole, sulfathiazole and sulfadiazine. Furthermore, the fabricated sensor is successfully applied to quantitatively determine and analyze SN present in spiked human serum and ground water samples.

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Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of Sulfanilamide Using Pencil Graphite Electrode Based on Molecular Imprinting Technology

Tadi¹,

Motghare²,

Ganesh³

2014

Electroanalysis

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“…In the last 10 years, a number of FPIA methods for herbicides and veterinary drugs in vegetable, fruits and animal tissues were developed (Sanchez et al. , 1993; Eremin et al. , 1994, 2002, 2005; Nistor et al.…”

Section: Introductionmentioning

confidence: 99%

Determination of the veterinary drug maduramicin in food by fluorescence polarisation immunoassay

Wang

Zhang

Murtazina

et al. 2007

Int J of Food Sci Tech

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A fluorescence polarisation immunoassay (FPIA) using a specific polyclonal antiserum for the detection of maduramicin (MD) was developed and optimised. The polyclonal antiserum was produced against MD linked to bovine serum albumin. Fluorescein-labelled MD (tracer) was synthesised by N-hydroxysuccinimide active ester method and purified using thin layer chromatography. The developed FPIA for MD had a dynamic range from 0.01 to 5.6 lg mL )1 with an IC 50 value of 0.16 lg mL )1 and a limit of detection of 0.002 lg mL )1 . Recoveries from chicken muscle, fat and egg samples spiked at 0.25, 5 and 10 lg g )1 levels were 82-130%. The FPIA results from analysis of incurred residues in chicken muscle samples showed that the simple procedure is viable.

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“…Polyclonal antibodies against SAs were produced as previously described [54]. Briefly, male brush rabbits at the age of 3-5 months were immunized every 2 weeks with the conjugate of bovine serum albumin and the corresponding SA (0.5-1.0 mg/mL) mixed at equal volume with complete Freund's adjuvant for the first immunization and with incomplete Freund's adjuvant for subsequent immunizations.…”

Section: Obtaining Antibodiesmentioning

confidence: 99%

Changing Cross-Reactivity for Different Immunoassays Using the Same Antibodies: Theoretical Description and Experimental Confirmation

et al. 2021

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Many applications of immunoassays involve the possible presence of structurally similar compounds that bind with antibodies, but with different affinities. In this regard, an important characteristic of an immunoassay is its cross-reactivity: the possibility of detecting various compounds in comparison with a certain standard. Based on cross-reactivity, analytical systems are assessed as either high-selective (responding strictly to a specific compound) or low-selective (responding to a number of similar compounds). The present study demonstrates that cross-reactivity is not an intrinsic characteristic of antibodies but can vary for different formats of competitive immunoassays using the same antibodies. Assays with sensitive detection of markers and, accordingly, implementation at low concentrations of antibodies and modified (competing) antigens are characterized by lower cross-reactivities and are, thus, more specific than assays requiring high concentrations of markers and interacting reagents. This effect was confirmed by both mathematical modeling and experimental comparison of an enzyme immunoassay and a fluorescence polarization immunoassay of sulfonamides and fluoroquinolones. Thus, shifting to lower concentrations of reagents decreases cross-reactivities by up to five-fold. Moreover, the cross-reactivities are changed even in the same assay format by varying the ratio of immunoreactants’ concentrations and shifting from the kinetic or equilibrium mode of the antigen-antibody reaction. The described patterns demonstrate the possibility of modulating immunodetection selectivity without searching for new binding reactants.

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Production of Polyclonal Antibodies and Development of Fluorescence Polarization Immunoassay for Sulfanilamide

Cited by 37 publications

References 29 publications

Electrochemical Detection of Sulfanilamide Using Pencil Graphite Electrode Based on Molecular Imprinting Technology

Electrochemical Detection of Sulfanilamide Using Pencil Graphite Electrode Based on Molecular Imprinting Technology

Determination of the veterinary drug maduramicin in food by fluorescence polarisation immunoassay

Changing Cross-Reactivity for Different Immunoassays Using the Same Antibodies: Theoretical Description and Experimental Confirmation

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