Production of Zinc and Copper as Nanoparticles by Green Synthesis Using Pseudomonas fluorescens

Fawzy, Mohamed Hesham; Mahmoud, Sara Mohamed; Hanafy, Mohamed Ahmed; Bakr, Mohamed Hassan; Mahmoud, A.E.M.; Ali, Mohamed A.; Barakat, Olfat S.

doi:10.3923/pjbs.2021.445.453

Cited by 6 publications

(1 citation statement)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The powder of M-CuO NPs (10 mg/ml) was suspended in water, and the absorption peaks in the ultraviolet range at 300-390 nm with 3.5 intensity and the other peak in the visible range at 620-800 nm with an intensity of 1.0 were recorded (Figure 2B). Hesham et al (2021) revealed the peak of 300 nm for CuO NPs synthesized from cell-free supernatants of Pseudomonas fluorescens. Frontiers in Chemistry frontiersin.org Tiwari et al (2016) also obtained absorption at a wavelength in the 300-350 nm range and another peak at 570-630 nm of CuO NPs synthesized from cell-free filtrate of Bacillus cerus SWSD1.…”

Section: Uv-visible Resultsmentioning

confidence: 99%

Biocontrol potential of mycogenic copper oxide nanoparticles against Alternaria brassicae

et al. 2022

View full text Add to dashboard Cite

The biological synthesis of nanoparticles using fungal cultures is a promising and novel tool in nano-biotechnology. The potential culture of Trichoderma asperellum (T. asperellum) has been used to synthesize copper oxide nanoparticles (CuO NPs) in the current study. The necrotrophic infection in Brassica species is caused due to a foliar pathogen Alternaria brassicae (A. brassicae). Mycogenic copper oxide nanoparticles (M-CuO NPs) were characterized by spectroscopic and microscopic techniques such as UV–visible spectrophotometry (UV–vis), transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The antifungal potential of CuO NPs was studied against A. brassicae. M-CuO NPs exhibited a surface plasmon resonance (SPR) at 303 nm, and XRD confirmed the crystalline phase of NPs. FTIR spectra confirmed the stretching of amide bonds, and the carbonyl bond indicated the presence of enzymes in T. asperellum filtrate. SEM and TEM confirmed the spherical shape of M-CuO NPs with an average size of 22 nm. Significant antifungal potential of M-CuO NPs was recorded, as it inhibited the growth of A. brassicae up to 92.9% and 80.3% in supplemented media with C-CuO NPs at 200 ppm dose. Mancozeb and propiconazole inhibited the radial growth up to 38.7% and 44.2%. SEM confirmed the morphological changes in hyphae and affected the sporulation pattern. TEM revealed hardly recognizable organelles, abnormal cytoplasmic distribution, and increased vacuolization, and light microscopy confirmed the conidia with reduced diameter and fewer septa after treatment with both types of NPs. Thus, M-CuO NPs served as a promising alternative to fungicides.

show abstract

Section: Uv-visible Resultsmentioning

confidence: 99%