Production optimization, partial characterization and properties of an exopolysaccharide from Lactobacillus sakei L3

Wang, Binbin; Song, Qiaozhi; Zhao, Fangkun; Han, Yongdian; Zhou, Zhansong

doi:10.1016/j.ijbiomac.2019.08.241

Cited by 40 publications

(25 citation statements)

References 44 publications

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“…bulgaricus NCFB 2483 obtained by elemental analysis were 39.1, 6.2, 2.8, and 50.3, respectively. In the study of Wang et al 40 , the amounts of C, H, N, and S in crude L3 EPS (C-EPS) from L. sakei L3 obtained by elemental analysis were 39.3, 6.1, 0.4, and 0.1, respectively. Usually, polysaccharide-rich samples do not contain or show only small amounts of N 41 .…”

Section: Resultsmentioning

confidence: 99%

Characterization of lactic acid bacteria derived exopolysaccharides for use as a defined neuroprotective agent against amyloid beta1–42-induced apoptosis in SH-SY5Y cells

Şirin

Aslım

2020

Sci Rep

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Alzheimer's disease (AD) is a disease characterized by cerebral neuronal degeneration and loss in a progressive manner. Amyloid beta (Aβ) in the brain is toxic to neurons, being a main risk factor for initiation and continuation of cognitive deterioration in AD. Neurotoxicity of Aβ origin is also linked to oxidative stress characterized by excessive lipid peroxidation, protein oxidation, changes in antioxidant systems, and cerebral DNA damage in AD. Furthermore, Aβ can induce oxidative neuronal cell death by a mitochondrial dysfunction. Cellular injury caused by oxidative stress can be possibly prevented by boosting or promoting bodily oxidative defense system by supplying antioxidants in diet or as medications. However, most synthetic antioxidants are found to have cytotoxicity, which prevents their safe use, and limits their administration. For this reason, more attention has been paid to the natural non-toxic antioxidants. One of the most promising groups of non-toxic antioxidative compounds is thought to be polysaccharides. This study investigated the characterization and protective action exerted by exopolysaccharides (EPSs) originated from Lactobacillus delbrueckii ssp. bulgaricus B3 and Lactobacillus plantarum GD2 to protect from apoptotic activity exerted by Aβ 1-42 among SH-SY5Y cells. We characterized EPSs by elemental analysis, FTIR, AFM, SEM, and XRD. The antioxidant effects of EPSs were determined by the DPPH free radical scavenging activity, hydroxyl radical scavenging activity, metal ion chelating activity, lipid peroxidation inhibitory activity, and superoxide anion scavenging activity method. The protective effects of EPSs were determined by flow cytometry and RT-PCR. Mannose ratio, molecular weight, functional groups, surface morphology, and amorphous character structure of EPSs are thought to play a role in the protective effect of EPSs. EPSs reduced apoptotic activity of Aβ 1-42 in addition to their depolarizing effect on mitochondrial membrane potential in concentration-dependent manner. These observations contribute the inclusion of EPSs among the therapeutic options used to manage various neurological disorders in the traditional medicine in a scientific manner, indicating that EPSs may be promising natural chemical constituents that need advanced research and development for pharmacological therapy of AD. Alzheimer's disease (AD) is a disease characterized by cerebral neuronal degeneration and loss in a progressive manner; these events show parallelism with two most striking neuropathological signs of AD, i.e. the formation of neurofibrillary tangles and senile plaques 1. Amyloid beta (Aβ) fibrils are formed in multiple steps, which follow the monomeric Aβs being oligomerized and aggregated, where the peptide goes through a conformational change from an alpha-helical to a beta-pleated sheet architecture. It has been recently shown that cerebral neurotoxicity arises from the effects of soluble oligomers of Aβ 2. Multiple studies both performed in vitro and in vivo have demonstrated that Aβ ...

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Section: Resultsmentioning

confidence: 99%

Characterization of lactic acid bacteria derived exopolysaccharides for use as a defined neuroprotective agent against amyloid beta1–42-induced apoptosis in SH-SY5Y cells

Şirin

Aslım

2020

Sci Rep

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“…The Plackett Burman design is an essential tool in screening the effects of process variables. Further, it is frequently used to enhance the product yield, reduces the process variability and number of experiments, process time and overall cost [21][22][23]. The Response surface methodology (RSM) with Box Behnken design is a widely used mathematical optimization tool due to simultaneous analysis of interactions of several parameters with the minimum of experimental trials.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Characterization of cardinal vine shoot waste as new resource of lignocellulosic biomass and valorization into value-added chemical using Plackett–Burman and Box Behnken

Amine

Amrane

Aksil

et al. 2021

Biomass Conv. Bioref.

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The objective of this work was to valorize a waste from cardinal vine shoot into a hydro-lysate rich in reducing sugars. Plackett-Burman design was considered to identify the significant factors while a Box Behnken design was considered to optimize the extraction in the following experimental conditions: 100 °C, 750 rpm, trifluoracetic acid (CF3O2H) concentration (TFA) in the range (1 -10%), during 20 to 180 min and considering the following solid-liquid (S/V) ratios (1:1, 3:1, 5:1). The optimal result was 2.53% in sugars equivalent to a yield of 50.64% per g of dry matter. Shoot vine waste was characterized by Attenuated Total Reflection-Fourier Transform Infrared

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“…GUMS16 may be indicative of its commercial potential since its EPS yield exceeded the reported optimal EPS yields of 2.2 g/100 mL, 11.8 g/100 mL and 12.6 g/100 mL generated from Bacillus mucilaginosus CGMCC5766, Cupriavidus pauculus KPS 201 and Spirulina Platensis, respectively, reported in the literature [47][48][49]. The total sugar content of the optimally generated EPS was also determined using the phenol sulfuric acid method [50,51]. It was determined that the mean sugar content was 60% mass basis and was comparable to the sugar content of EPS reported in a previous work that ranges from 34-71% mass basis [52].…”

Section: Effect Of Sucrose Concentrationmentioning

confidence: 99%

Optimization of Exopolysaccharide (EPS) Production by Rhodotorula mucilaginosa sp. GUMS16

et al. 2021

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Exopolysaccharides (EPSs) are important biopolymers with diverse applications such as gelling compounds in food and cosmetic industries and as bio-flocculants in pollution remediation and bioplastics production. This research focuses on enhancing crude EPS production from Rhodotorula mucilaginosa sp. GUMS16 using the central composite design method in which five levels of process variables of sucrose, pH, and ammonium sulfate were investigated with sucrose and ammonium sulfate serving as carbon and nitrogen sources during microbial incubation. The optimal crude EPS production of 13.48 g/100 mL was achieved at 1 g/100 mL of sucrose concentration, 14.73 g/100 mL of ammonium sulfate at pH 5. Variations in ammonium sulfate concentrations (1.27–14.73 g/100 mL) presented the most significant effects on the crude EPS yield, while changes in sucrose concentrations (1–5 g/100 mL) constituted the least important process variable influencing the EPS yield. The Rhodotorula mucilaginosa sp. GUMS16 may have the potential for large-scale production of EPS for food and biomedical applications.

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Production optimization, partial characterization and properties of an exopolysaccharide from Lactobacillus sakei L3

Cited by 40 publications

References 44 publications

Characterization of lactic acid bacteria derived exopolysaccharides for use as a defined neuroprotective agent against amyloid beta1–42-induced apoptosis in SH-SY5Y cells

Characterization of lactic acid bacteria derived exopolysaccharides for use as a defined neuroprotective agent against amyloid beta1–42-induced apoptosis in SH-SY5Y cells

Characterization of cardinal vine shoot waste as new resource of lignocellulosic biomass and valorization into value-added chemical using Plackett–Burman and Box Behnken

Optimization of Exopolysaccharide (EPS) Production by Rhodotorula mucilaginosa sp. GUMS16

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