Production, Purification, and Biochemical Characterization of Thermostable Metallo-Protease from Novel Bacillus alkalitelluris TWI3 Isolated from Tannery Waste

Anandharaj, Marimuthu; Sivasankari, B.; Siddharthan, Nagarajan; Rani, Rizwana Parveen; Sivakumar, S.

doi:10.1007/s12010-015-1974-7

Cited by 37 publications

(16 citation statements)

References 34 publications

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“…The genus Bacillus , belonging to Firmicutes , was the dominant bacterium in both composts with a relative abundance of 16% ( Figure 3 ). Bacillus is well known for the production of thermostable proteases ( Jellouli et al, 2011 ; Anandharaj et al, 2016 ), and strains belonging to this genus have been isolated from manure compost previously ( Charbonneau et al, 2012 ). Bacillus might therefore play an important role in the degradation of nitrogen sources such as chicken manure.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Growth and Activities of the Dominant Functional Microbiota of Chicken Manure Composts in the Presence of Maize Straw

Zhang

Pan

et al. 2018

Front. Microbiol.

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As a consequence of intensive feeding, the bulk deposition of livestock manure causes severe environmental problems. Composting is a promising method for waste disposal, and the fermentation process is driven by microbial communities. However, chicken manure contains diverse gut microbes, mainly species derived from Proteobacteria, which may include pathogens that threaten human health. To evaluate composting as a harmless treatment of livestock manure, the dynamics of the microbiota in two chicken manure composts were studied, and the influences of adding maize straw on the compost microbiota were compared. The results revealed that microbes from Firmicutes including Bacillus and Lentibacillus are the most dominant degraders with a strong amino acid metabolism, and they secrete a diverse array of proteases as revealed in metaproteomics data. The addition of maize straw to the chicken manure compost accelerated species succession at the initial stage, and stimulated carbohydrate metabolism in the dominant microbiota. Besides, under the resulting high temperature (>70°C) conditions, the relative abundance of Proteobacteria was reduced by 78% in composts containing maize straw by day 4, which was faster than in compost without added maize straw, in which the abundance was reduced by 66%. Adding maize straw to chicken manure composts can therefore increase the fermentation temperature and inhibit the growth of Proteobacteria. In general, these findings provide increased insight into the dynamic changes among the dominant functional microbiota in chicken manure composts, and may contribute to the optimization of livestock manure composting on an industrial scale.

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Section: Discussionmentioning

confidence: 99%

Enhanced Growth and Activities of the Dominant Functional Microbiota of Chicken Manure Composts in the Presence of Maize Straw

Zhang

Pan

et al. 2018

Front. Microbiol.

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“…Keratinolytic enzymes can even contribute to improving drug delivery through the skin (epidermis) ( Gupta et al, 2013a ). Indeed, keratinolytic enzymes have been added as supplements to cosmetics for use in skin whitening and in exfoliation and for assisting drug permeation ( Anandharaj et al, 2016 ; Gupta et al, 2013a ). Keratinolytic enzymes formulated in dehairing cream have also been reported to be particularly effective in removing hair compared to conventional marketed formulations ( Sanghvi et al, 2016 ).…”

Section: Applications Of Keratinolytic Enzymesmentioning

confidence: 99%

Microbial enzymes catalyzing keratin degradation: Classification, structure, function

Qiu

Wilkens

Barrett

et al. 2020

Biotechnology Advances

157

103

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Keratin is an insoluble and protein-rich epidermal material found in e.g. feather, wool, hair. It is produced in substantial amounts as co-product from poultry processing plants and pig slaughterhouses. Keratin is packed by disulfide bonds and hydrogen bonds. Based on the secondary structure, keratin can be classified into α-keratin and β-keratin. Keratinases (EC 3.4.-.- peptide hydrolases) have major potential to degrade keratin for sustainable recycling of the protein and amino acids. Currently, the known keratinolytic enzymes belong to at least 14 different protease families: S1, S8, S9, S10, S16, M3, M4, M14, M16, M28, M32, M36, M38, M55 (MEROPS database). The various keratinolytic enzymes act via endo -attack (proteases in families S1, S8, S16, M4, M16, M36), exo -attack (proteases in families S9, S10, M14, M28, M38, M55) or by action only on oligopeptides (proteases in families M3, M32), respectively. Other enzymes, particularly disulfide reductases, also play a key role in keratin degradation as they catalyze the breakage of disulfide bonds for better keratinase catalysis. This review aims to contribute an overview of keratin biomass as an enzyme substrate and a systematic analysis of currently sequenced keratinolytic enzymes and their classification and reaction mechanisms. We also summarize and discuss keratinase assays, available keratinase structures and finally examine the available data on uses of keratinases in practical biorefinery protein upcycling applications.

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“…The enzyme with high activity was assumed to be 100% active. All experiments were performed in triplicates (Anandharaj et al, 2016).…”

Section: Effect Of Temperature On Protease Stability and Activitymentioning

confidence: 99%

Isolation and identification of alkaline protease-producing bacterium in the presence of washing-powder from Geinarje hot spring, Ardabil, Iran

Taheri

Ebrahimipour

Sadeghi

2019

nbr

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Proteases are important industrial enzymes used in different areas of industry, mainly detergent, food and leather industries. In this study, novel alkaline protease-producing bacterium was isolated from Geinarje hot spring and examined for maximum protease activity to be utilized in washing-powder. The isolated bacterium was cultured in mineral salt medium including 2% Skim Milk. Proteolytic activity of supernatant was measured by caseinolytic method. The effects of pH, temperature, SDS, Tween 80 and EDTA on protease stability and activity were investigated. The detergent compatibility of protease was assayed. On the basis of phylogenetic analysis and morphological as well as biochemical tests, the isolate was identified as a new strain of Brevibacillus borstelensis capable of generating extracellular alkaline protease. The generated protease was determined as alkaline metallo-protease having high activity at 60 o C and pH 9. Moreover, the alkaline protease was stable in the presence of SDS, Tween 80 and H2O2. It is compatible with commercial detergents. Finding proteases capable of degrading proteins in extreme environment (i.e. alkaline pH, high temperature and presence of surfactants) is valuable in biotechnological and industrial practices. Therefore, it can be utilized in detergent formulation in the future.

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Production, Purification, and Biochemical Characterization of Thermostable Metallo-Protease from Novel Bacillus alkalitelluris TWI3 Isolated from Tannery Waste

Cited by 37 publications

References 34 publications

Enhanced Growth and Activities of the Dominant Functional Microbiota of Chicken Manure Composts in the Presence of Maize Straw

Enhanced Growth and Activities of the Dominant Functional Microbiota of Chicken Manure Composts in the Presence of Maize Straw

Microbial enzymes catalyzing keratin degradation: Classification, structure, function

Isolation and identification of alkaline protease-producing bacterium in the presence of washing-powder from Geinarje hot spring, Ardabil, Iran

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