Production, purification, and quantification of the reduced flavin-dependent monooxygenase MsuD from Pseudomonas fluorescens Pf0-1

Abstract

Background Biological assimilation of dimethylsulfone Production of MsuD• Sulfur is an essential element of life.• Bacteria can directly intake sulfur from the environment or break down organosulfur compounds into a form that they can incorporate to their systems. • The biological assimilation of dimethylsulfone can be achieved by a series of enzymatic reactions. • The final step of the pathway is the conversion of methanesulfonate to inorganic sulfite catalyzed by the enzyme MsuD. SDS-PAGE for MsuD Characterization of MsuD by mass spectrometryFuture Research• An endpoint assay will be run to verify if the MsuD enzyme is active.• The kinetic parameters for MsuD can be determined with a colorimetric assay for sulfite concentration by its reaction with 5,5dithio-bis-(2-nitrobenzoic acid) (DTNB).