Produksi Dan Ekstraksi Inhibitor Alfa Glukosidase Dari Isolat Aktinomiset Jp-3

Abstract

Alpha-glucosidase inhibitors are compounds that can prevent the digestion of complex carbohydrates into glucose, so potentially used as a diabetes drug. This study aims to examine the production and extraction of alpha-glucosidase inhibitor compound from Isolate Aktinomiset JP-3 from the sea. The supernatant obtained from the culture of the JP-3 isolate was extracted using various solvents to obtain the active compound. The solvents used were chloroform, methanol, and ethyl acetate. An assay of inhibitor activity of the α-glucosidase using p-nitrophenyl α-D-glucopyranoside substrate. The activity of the enzyme is measured based on the absorbance of p-nitrophenol produced from the breaking reaction of the substrate. The results showed that extraction of alpha-glucosidase inhibitor compound with ethyl acetate yielded extract with highest inhibitor activity. Keywords: alpha-glucosidase inhibitors, actinomycetes, diabetes, extraction, fractionation

Inhibitory activity of endophytic fungi against alpha-amylase isolated from raru (Cotylelobium melanoxylon)

“…The mixture was heated in boiling water for 15 min to stop the reaction, then the absorbance value was measured using an UV spectrophotometer vis at 540 nm. Akarbosa solution was used as a comparison solution (Pujiyanto et al, 2016). The test was carried out 2 times (Pujiyanto et al, 2016).…”

“…Akarbosa solution was used as a comparison solution (Pujiyanto et al, 2016). The test was carried out 2 times (Pujiyanto et al, 2016).…”

Screening of Lactobacillus Inhibitor Alpha Glucosidase Activity from Nira in Hyperglycemic Mice

“…Alfa glukosidae inhibitor adalah adalah satu jenis obat untuk diabetes yang bekerja memblok metabolisme pati dengan cara menghambat enzim dalam usus untuk memecah karbohidrat (early et al, 2013) cara kerja alfa inhibitor glukosidase memperlambat penyerapan glukosa (Pujiyanto & Siti, 2015). Pemberian alfaglukosidae inhibitor untuk menurunkan hiperglikemik (gula darah diatas normal) seperti biasanya diberikan dengan kombinasi obat lain jika metformin tidak memadai sehingga lebih cepat untuk menurunkan kadar glukosa darah (BPOM, 2021).…”

Miskonsepsi Konsep Archaebacteria Dan Eubacteria

“…Tabel 4. Miskonsepsi Utuh pada Butir Soal 1 Menurut teori dalam pustaka, bakteri merupakan organisme prokariotik dengan ciri khas selnya yang tidak memiliki membran inti (Pujiyanto, 2012). Miskonsepsi responden terhadap konsep struktur anatomis sel bakteri ini kemungkinan disebabkan responden tidak memiliki pengetahuan prasarat (konsep sel secara umum) pada saat mengikuti pembelajaran konsep struktur anatomis sel bakteri (Tarwoko, 2005).…”

“…Secara teori, endospora adalah struktur pertahanan didalam sel bakteri yang dibentuk saat kondisi tidak menguntungkan (Pujiyanto, 2012). Penggunaan kata melindungi dalam menjelaskan fungsi endospora menyiratkan adanya kesalahpahaman siswa.…”