Profenofos induced DNA damage in freshwater fish, Channa punctatus (Bloch) using alkaline single cell gel electrophoresis

Pandey, A. K.; Nagpure, N. S.; Trivedi, Sunil P.; Kumar, Ravindra; Kushwaha, Basdeo

doi:10.1016/j.mrgentox.2011.09.011

Cited by 66 publications

(38 citation statements)

References 68 publications

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“…These results are in line with previous studies on genotoxicity as higher values for different comet parameters were also reported by Ali and Kumar (2008) in C. punctatus after exposure to monocrotophos, Ateeq et al (2005) in C. batrachus in response to 2,4-dichlorophenoxyacetic acid and butachlor, Pandey et al (2011) in C. punctatus after its treatment with profenfos, and Guilherme et al (2012) in Anguilla anguilla when it was exposed to glyphosate-based herbicide.…”

Section: Discussionsupporting

confidence: 92%

Widely used non-ionic surfactant 4-nonylphenol: showing genotoxic effects in various tissues of Channa punctatus

Sharma

Chadha

2017

Environ Sci Pollut Res

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The present study investigated the intertissue differences in genotoxicity induction in the fish, Channa punctatus, in response to 4-nonylphenol. The lethal concentration of 50 (LC) was estimated using a semi-static system. Fish were subjected to three sublethal concentrations of 4-nonylphenol (NP) for 24, 48, 72, and 96 h. Liver, gill, and kidney tissues were analyzed, and the genotoxicity was estimated using the micronucleus test and the comet assay. The frequency of micronucleated cells (MNCs), binucleated cells (BNCs), aberrant cells, and tail moment (TM) increased significantly. The time for maximum induction of genotoxicity for all the parameters considered in the micronucleus assay was 72 h of exposure, whereas the TM was highest at 24 h of exposure followed by a decline. The gill and liver showed higher genotoxicity than kidney tissue demonstrating organ-specific susceptibilities to NP in fish C. punctatus.

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Section: Discussionsupporting

confidence: 92%

Widely used non-ionic surfactant 4-nonylphenol: showing genotoxic effects in various tissues of Channa punctatus

Sharma

Chadha

2017

Environ Sci Pollut Res

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“…Still, tail length was, as a parameter, used in studies of genotoxicity in fish tissues [39][40][41][42]. Nevertheless, other parameters, especially, tail intensity and tail moment, were more extensively used in similar works [43][44][45]. Kumaravel and Jha [33] recommend that both OTM and % tail intensity could be used for scientific purposes.…”

Section: Discussionmentioning

confidence: 99%

Comparison of comet assay parameters for estimation of genotoxicity by sum of ranking differences

et al. 2013

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Genotoxic potential of waters in six rivers and reservoirs from Serbia was monitored in different tissues of chub (Squalius cephalus L. 1758) with the alkaline comet assay. The comet assay, or a single cell gel electrophoresis, has a wide application as a simple and sensitive method for evaluating DNA damage in fish exposed to various xenobiotics in the aquatic environment. Three types of cells, erythrocytes, gills and liver cells were used for assessing of DNA damage. Images of randomly selected cells were analyzed with a fluorescence microscope Leica and image analysis by software (Comet Assay IV Image analysis system, PI, UK). Three parameters (tail length -l, tail intensity -i and Olive tail moment -m) were analyzed on 1750 nuclei per cell type. The procedure for sum of ranking differences (SRD) was implemented to compare different types of cells and different parameters for estimation of DNA damage.Regarding our nine different estimations of genotoxicity: tail length, intensity and moment in erythrocytes (rel, rei, rem), liver cells (rll, rli, rlm) and gill cells (rgl, rgi, rgm) SRD procedure has shown that the Olive tail moment and tail intensity are (almost) equally good parameters; the SRD value was lower for the tail moment and tail intensity than for tail length in case of all types of cells. The least reliable parameter was rel; close to the borderline case were rei, rll, and rgl (~5% probability of random ranking).

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“…This data is lower than 2.35 and 3.17 mgL -1 reported for Anabas testudineus (Patar et al, 2015) and Aphanius iberus (Varó et al, 2008) but higher than 0.48 and 0.55mgL -1 in bluegill and spots respectively (Kenaga, 1979). Toxicity of compounds on aquatic biota has been documented to have effects on water quality, chemical formulations, age and size of the exposed species (Wekler, 2000;Pandey et al, 2011). The toxic effects of DDVP on C. gariepinus may have caused the mortality observed in the investigation.…”

Section: Behavioural Responses and Lc 50mentioning

confidence: 99%

“…Dichlorvos (dimethyl-2,2-dichlorovinyl phosphate) is an organophosphate (Ops) insecticide used against insect pests to stored products of plants and animals as well as outdoor and greenhouse fruits and vegetable crops. The aquatic environment is under a constant threat as a result of indiscriminate use of synthetic pesticides including dichlorvos (Cerejeira et al, 2003;Pandey et al, 2011), which enter through run off and in most cases, predisposes non-target organisms to potential toxicity. In the water, its molecules may accumulate in sediments or be absorbed by the aquatic organisms with attendant patho-physiological changes (Jordan et al, 2013).…”

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confidence: 99%

Butrylacetycholinesterase Activity in Liver and Plasma, Liver Glycogen and Plasma Glucose Content, Haematology and Behaviour of Clariid Catfish Clarias Gariepinus to Dichlorvos

Ezike

Felix

Ajima

et al. 2017

IJAFAS

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Semi-static bioassay experiment was conducted to ascertain Butrylacetycholinesterase enzyme activity in liver and plasma, liver glycogen and plasma glucose content, haematology as well as behaviour of Clarias gariepinus (mean weight 24.52±1.64 g) to varying concentrations (0, 0.4, 0.8, 1.6 mg L -1 ) of Dichlorvos for 96 hours. Butrylacetycholinesterase activity in liver was significantly (P <0.05) inhibited all through the exposure duration to 0.4, 0.8 and 1.6 mg L -1 of DDVP compared with control group of fish. Similarly, plasma BuChE activity was inhibited all through the exposure duration to 1.6 mg L -1 DDVP compared to control. There was a significant (P <0.05) inhibition in HB, RBC and MCV of exposed fish after 24,48, 72 and 96h to 1.6 mg L -1 DDVP compared to the control but PCV and WBC were significantly elevated after 72 and 96h to 1.6 mg L -1 DDVP compared to the control.Plasma cortisol and liver glycogen were significantly inhibited after 72 and 96 h to 0.8 and 1.6 mg L

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Profenofos induced DNA damage in freshwater fish, Channa punctatus (Bloch) using alkaline single cell gel electrophoresis

Cited by 66 publications

References 68 publications

Widely used non-ionic surfactant 4-nonylphenol: showing genotoxic effects in various tissues of Channa punctatus

Widely used non-ionic surfactant 4-nonylphenol: showing genotoxic effects in various tissues of Channa punctatus

Comparison of comet assay parameters for estimation of genotoxicity by sum of ranking differences

Butrylacetycholinesterase Activity in Liver and Plasma, Liver Glycogen and Plasma Glucose Content, Haematology and Behaviour of Clariid Catfish Clarias Gariepinus to Dichlorvos

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