Profiling of small RNA cargo of extracellular vesicles shed by Trypanosoma cruzi reveals a specific extracellular signature

Fernández-Calero, Tamara; Garcia-Silva, Rosa; Peña, Álvaro; Robello, Carlos; Persson, Helena; Rovira, Carlos; Naya, Hugo; Cayota, Alfonso

doi:10.1016/j.molbiopara.2015.03.003

Cited by 51 publications

(53 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bayer-Santos et al (2013), found two types of EVs secreted by the parasites. Later, Garcia-Silva et al (2014), Linhares-Lacerda et al (2015), andFernandez-Calero et al (2015) showed the involvement of miRNA in MV transfer to host cells, indicating this mechanism as a novel modulating effect against neighbouring cells.…”

Section: Trofozoites From Giardia Intestinalis Respond To Environmentmentioning

confidence: 97%

“…There are numerous examples in the literature of protozoa releasing large amounts of material into the extracellular space as a form of cellular communication with host cells (Garcia-Silva et al, 2014;Linhares-Lacerda et al, 2015;Fernandez-Calero et al, 2015;Cestari et al, 2012). In Giardia intestinalis, the diarrhoea and malabsorption could be a direct result of the interaction of the parasite with the intestinal epithelium which might be mediated by the parasite itself, or by substances it secretes or by MVs that could alter neighbouring cells.…”

Section: Exosome and Microvesicles Content Can Alter Neighbouring Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Microvesicles released from Giardia intestinalis disturb host-pathogen response in vitro

Evans-Osses

Mojoli

Monguió‐Tortajada

et al. 2017

European Journal of Cell Biology

View full text Add to dashboard Cite

a b s t r a c tGiardia intestinalis (G.I), is an anaerobic protozoan and the aetiological agent of giardiasis, a diarrhoea present worldwide and associated with poverty. G.I has a simple life cycle alternating between cyst and trophozoite. Cysts are transmitted orally to the stomach and transform to trophozoites in the intestine by a multifactorial process. Recently, microvesicles (MVs) have been found to be released from a wide range of eukaryotic cells. We have observed a release of MVs during the life cycle of G.I., identifying MVs from active trophozoites and from trophozoites differentiating to the cyst form. The aim of the current work was to investigate the role of MVs from G.I in the pathogenesis of giardiasis. MVs from log phase were able to increase the attachment of G. intestinalis trophozoites to Caco-2 cells. Moreover, MVs from G. intestinalis could be captured by human immature dendritic cells, resulting in increased activation and allostimulation of human dendritic cells. Lipid rafts participate in the MV biogenesis and in the attachment to Caco-2 cells. Nevertheless, proteomic analysis from two types of MVs has shown slight differences at the protein levels. An understanding of biogenesis and content of MVs derived from trophozoites might have important implications in the pathogenesis of the disease.

show abstract

Section: Trofozoites From Giardia Intestinalis Respond To Environmentmentioning

confidence: 97%

Section: Exosome and Microvesicles Content Can Alter Neighbouring Cellsmentioning

confidence: 99%

Microvesicles released from Giardia intestinalis disturb host-pathogen response in vitro

Evans-Osses

Mojoli

Monguió‐Tortajada

et al. 2017

European Journal of Cell Biology

View full text Add to dashboard Cite

show abstract

“…In this respect, it was recently reported that T. cruzi trypomastigotes invade 5-fold as many susceptible cells when pre-incubated with purified parasite extracellular vesicles [53]. These results suggest that secreted vesicles from T. cruzi and their cargo could act as virulence factors by promoting metacyclogenesis and enhancing host cell susceptibility or both [53].…”

Section: Exosomes In the Protozoan Trypanosoma Cruzimentioning

confidence: 87%

“…These results suggest that secreted vesicles from T. cruzi and their cargo could act as virulence factors by promoting metacyclogenesis and enhancing host cell susceptibility or both [53]. T. cruzi virulence factors include molecules expressed on the cell surface as well as those secreted or shed into the extracellular medium.…”

Section: Exosomes In the Protozoan Trypanosoma Cruzimentioning

confidence: 93%

Exosomes in the Pathogenic Protozoan Trypanosoma Cruzi

Souza¹

2017

Int J Pathol Clin Res

View full text Add to dashboard Cite

Significant advances have recently occurred in the identification and characterization of different types of microvesicles released by eukaryotic cells into the extracellular space. Exosomes are one such type of these vesicles they originate from multivesicular bodies and have received much attention. Here, we review the available data on microvesicles secreted by the pathogenic protozoan Trypanosoma cruzi, which is the causative agent of Chagas disease and that still has a high prevalence in Latin America. The available data show that T. cruzi exosomes contain some key proteins and RNA molecules that contain genetic information and may interfere with transcription. The available information suggests that they play some role in the biology of T. cruzi, including its interaction with host cells, as well as on the pathogenesis of Chagas disease where inflammatory processes and host cell dead occurs as a consequence of parasites, factors released by the parasites, and molecules produced by different host cells that are part of the immunological response.

show abstract

“…Although it has been suggested that some virulence proteins are exported from pathogenic fungi to host plants via extracellular vesicles [89], definitive evidence remains to be presented. Studies of non-plant systems suggest that these vesicles act to export functional nucleic acids, including RNAs, between cells [90], and new data suggest that this includes other organisms (e.g., [64][65][66]69,91]). …”

Section: Mechanism Of Rna Movement Between Fungi and Plantsmentioning

confidence: 99%