Profiling of sperm gene transcripts in crossbred ( Bos taurus x Bos indicus ) bulls

Yathish, H.M.; Kumar, Subodh; Dubey, P. P.; Modi, Rajendra P.; Chaudhary, Rajni; Kumar, Anuj; Ghosh, Subrata; Sarkar, Mihir; Balasubramaniam, Sivamani

doi:10.1016/j.anireprosci.2016.12.003

Cited by 19 publications

(12 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regarding genes associated with IE, BMP4 and CDKN3 genes (chromosome 17) are annotated to embryo development processes in mice (Goggolidou et al, 2013). In chromosome 11, we found the CCT5 gene related to sperm quality in bulls (Yathish et al, 2017). Finally, the genes annotated for OR did not have a direct relationship with this trait or the female reproductive physiology.…”

Section: Gene Search and Functional Annotationsmentioning

confidence: 77%

A genomewide association study in divergently selected lines in rabbits reveals novel genomic regions associated with litter size traits

Sosa-Madrid

Santacreu

Blasco

et al. 2019

J Animal Breeding Genetics

View full text Add to dashboard Cite

Uterine capacity (UC), defined as the total number of kits from unilaterally ovariectomized does at birth, has a high genetic correlation with litter size. The aim of our research was to identify genomic regions associated with litter size traits through a genomewide association study using rabbits from a divergent selection experiment for UC. A high‐density SNP array (200K) was used to genotype 181 does from a control population, high and low UC lines. Traits included total number born (TNB), number born alive (NBA), number born dead, ovulation rate (OR), implanted embryos (IE) and embryo, foetal and prenatal survivals at second parity. We implemented the Bayes B method and the associations were tested by Bayes factors and the percentage of genomic variance (GV) explained by windows. Different genomic regions associated with TNB, NBA, IE and OR were found. These regions explained 7.36%, 1.27%, 15.87% and 3.95% of GV, respectively. Two consecutive windows on chromosome 17 were associated with TNB, NBA and IE. This genomic region accounted for 6.32% of GV of TNB. In this region, we found the BMP4, PTDGR, PTGER2, STYX and CDKN3 candidate genes which presented functional annotations linked to some reproductive processes. Our findings suggest that a genomic region on chromosome 17 has an important effect on litter size traits. However, further analyses are needed to validate this region in other maternal rabbit lines.

show abstract

Section: Gene Search and Functional Annotationsmentioning

confidence: 77%

A genomewide association study in divergently selected lines in rabbits reveals novel genomic regions associated with litter size traits

Sosa-Madrid

Santacreu

Blasco

et al. 2019

J Animal Breeding Genetics

View full text Add to dashboard Cite

show abstract

“…However, further evidence is required for full appreciation of this variation at transcriptomic level across species. Alternatively, the MSPS described here could also be used for other applications such as diagnostics [60], genetic characterization [61], nucleus-cytoplasm interactions [62], and interspecies cell-fusion or nuclear transfer experiments [56,63].…”

Section: Discussionmentioning

confidence: 99%

Inter-genus gene expression analysis in livestock fibroblasts using reference gene validation based upon a multi-species primer set

et al. 2019

View full text Add to dashboard Cite

Quantitative reverse transcription PCR (RT-qPCR) remains as an accurate approach for gene expression analysis but requires labor-intensive validation of reference genes using species-specific primers. To ease such demand, the aim was to design and test a multi-species primer set to validate reference genes for inter-genus RT-qPCR gene expression analysis. Primers were designed for ten housekeeping genes using transcript sequences of various livestock species. All ten gene transcripts were detected by RT-PCR in Bos taurus (cattle), Bubalus bubalis (buffaloes), Capra hircus (goats), and Ovis aries (sheep) cDNA. Primer efficiency was attained for eight reference genes using B . taurus—O . aries fibroblast cDNA (95.54–98.39%). The RT-qPCR data normalization was carried out for B . taurus vs. O . aries relative gene expression using Bestkeeper, GeNorm, Norm-finder, Delta CT method, and RefFinder algorithms. Validation of inter-genus RT-qPCR showed up-regulation of TLR4 and ZFX gene transcripts in B . taurus fibroblasts, irrespectively of normalization conditions (two, three, or four reference genes). In silico search in mammalian transcriptomes showed that the multi-species primer set is expected to amplify transcripts of at least two distinct loci in 114 species, and 79 species would be covered by six or more primers. Hence, a multi-species primer set allows for inter-genus gene expression analysis between O . aries and B . taurus fibroblasts and further reveals species-specific gene transcript abundance of key transcription factors.

show abstract

“…Therefore, to obtain a reliable profiling of TNP mRNA, more number of bulls with contrasting semen quality is essential. Profiling of other candidate genes of sperm motility was performed in Vrindavani bulls (Sivakumar, 2014;Yathish, 2015;Yathish et al, 2017Yathish et al, , 2018Yathish, Kumar, Sivakumar, Dubey, & Ghosh, 2014).…”

Section: Expression Profiling Of Tnps Genesmentioning

confidence: 99%

Identification of sperm motility markers in bovine transition protein genes

Maheshwarappa

Kumar

Chaudhary

et al. 2018

Reprod Domestic Animals

Self Cite

View full text Add to dashboard Cite

Contents Transition proteins (TNPs) are essential in chromatin condensation during spermiogenesis, and hence, they are the candidate genes for identifying sperm motility markers. Coding and in silico predicted promoter regions of these genes were investigated in crossbred and purebred cattle, and also, their mRNA quantification was done to explore its use as a diagnostic tool of infertility. PCR‐SSCP analysis revealed two band patterns in fragment III of TNP1 and fragment II of TNP2 gene. Sequence analysis revealed a deletion of “G” nucleotide in 3′UTR region of TNP1 and C>T SNP in intronic region of TNP2 gene. Least square analysis of variance did not reveal any significant influence of nucleotide deletion on any sperm motility parameters in both crossbred and purebred cattle. However, C>T SNP had a significant effect on initial progressive motility (p < 0.05) in purebred cattle and post‐thaw motility in overall cattle population. RT‐qPCR analysis did not reveal any significant variation in TNP1 and TNP2 gene expression among poorly motile and good quality spermatozoa of Vrindavani bulls.

show abstract

Profiling of sperm gene transcripts in crossbred ( Bos taurus x Bos indicus ) bulls

Cited by 19 publications

References 45 publications

A genomewide association study in divergently selected lines in rabbits reveals novel genomic regions associated with litter size traits

A genomewide association study in divergently selected lines in rabbits reveals novel genomic regions associated with litter size traits

Inter-genus gene expression analysis in livestock fibroblasts using reference gene validation based upon a multi-species primer set

Identification of sperm motility markers in bovine transition protein genes

Contact Info

Product

Resources

About