Profiling the proteoforms of urinary prostate-specific antigen by capillary electrophoresis – mass spectrometry

Moran, Alan B.; Domínguez‐Vega, Elena; Nouta, Jan; Pongrácz, Tamás; Reijke, Theo M. de; Wuhrer, Manfred; Lageveen-Kammeijer, Guinevere S. M.

doi:10.1016/j.jprot.2021.104148

Cited by 19 publications

(47 citation statements)

References 40 publications

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“…As shown in the recent review [237], CE with UV or MS detection is capable to separate positional and linkage isomers of glycopeptides and glycans generated by enzymatic cleavage of glycosylated proteins. This is very important because characterization of all the isomeric forms of glycans and glycopeptides is relevant for monitoring of glycoprotein‐ and glycosylation‐related diseases, for example, prostate cancer [238].…”

Section: Discussionmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Kašička

2021

Electrophoresis

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The review provides a comprehensive overview of developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, microscale isolation, and physicochemical characterization of peptides from 2019 up to approximately the middle of 2021. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis, such as sample preparation, sorption suppression, EOF control, and detection, are presented. New developments in the individual CE and CEC methods are demonstrated and several types of their applications are shown. They include qualitative and quantitative analysis, determination in complex biomatrices, monitoring of chemical and enzymatic reactions and physicochemical changes, amino acid, sequence, and chiral analyses, and peptide mapping of proteins. In addition, micropreparative separations and determination of significant physicochemical parameters of peptides by CE and CEC methods are described.

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Section: Discussionmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Kašička

2021

Electrophoresis

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“…IACE can be an excellent problem-solving technique, providing a new approach to monitor molecular changes throughout the evolution of a disease and through an assessment of each post-translational modification. Every modified protein component of a proteoform can change as the disease progressed, in particular the glycosylation pattern [62,200,[204][205][206].…”

Section: Advantages Of Immunoaffinity Capillary Electrophoresis For the Detection Of Proteoformsmentioning

confidence: 99%

Immunoaffinity Capillary Electrophoresis in the Era of Proteoforms, Liquid Biopsy and Preventive Medicine: A Potential Impact in the Diagnosis and Monitoring of Disease Progression

Guzman¹,

Guzman

2021

Biomolecules

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Over the years, multiple biomarkers have been used to aid in disease screening, diagnosis, prognosis, and response to therapy. As of late, protein biomarkers are gaining strength in their role for early disease diagnosis and prognosis in part due to the advancements in identification and characterization of a distinct functional pool of proteins known as proteoforms. Proteoforms are defined as all of the different molecular forms of a protein derived from a single gene caused by genetic variations, alternative spliced RNA transcripts and post-translational modifications. Monitoring the structural changes of each proteoform of a particular protein is essential to elucidate the complex molecular mechanisms that guide the course of disease. Clinical proteomics therefore holds the potential to offer further insight into disease pathology, progression, and prevention. Nevertheless, more technologically advanced diagnostic methods are needed to improve the reliability and clinical applicability of proteomics in preventive medicine. In this manuscript, we review the use of immunoaffinity capillary electrophoresis (IACE) as an emerging powerful diagnostic tool to isolate, separate, detect and characterize proteoform biomarkers obtained from liquid biopsy. IACE is an affinity capture-separation technology capable of isolating, concentrating and analyzing a wide range of biomarkers present in biological fluids. Isolation and concentration of target analytes is accomplished through binding to one or more biorecognition affinity ligands immobilized to a solid support, while separation and analysis are achieved by high-resolution capillary electrophoresis (CE) coupled to one or more detectors. IACE has the potential to generate rapid results with significant accuracy, leading to reliability and reproducibility in diagnosing and monitoring disease. Additionally, IACE has the capability of monitoring the efficacy of therapeutic agents by quantifying companion and complementary protein biomarkers. With advancements in telemedicine and artificial intelligence, the implementation of proteoform biomarker detection and analysis may significantly improve our capacity to identify medical conditions early and intervene in ways that improve health outcomes for individuals and populations.

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“…In contrast, high sensitivity is of relevance for complex mixtures such as plasma or serum, especially when the targets are glycoproteins that are present at low concentrations. For example, a recent publication investigated the various proteoform variants of prostate‐specific antigen (PSA) present in urine of prostate cancer patients (Moran et al, 2021 ). Next to PSA glycoforms, six proteolytic cleavage variants could be identified on an intact level (Figure 8 ).…”

Section: Ms Glycomics: Integrated Workflowsmentioning

confidence: 99%

High sensitivity glycomics in biomedicine

Lageveen-Kammeijer

Küster

Reusch

et al. 2021

Mass Spectrometry Reviews

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Many analytical challenges in biomedicine arise from the generally high heterogeneity and complexity of glycan-and glycoconjugate-containing samples, which are often only available in minute amounts. Therefore, highly sensitive workflows and detection methods are required. In this review mass spectrometric workflows and detection methods are evaluated for glycans and glycoproteins. Furthermore, glycomic methodologies and innovations that are tailored for enzymatic treatments, chemical derivatization, purification, separation, and detection at high sensitivity are highlighted. The discussion is focused on the analysis of mammalian N-linked and GalNAc-type O-linked glycans.

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Profiling the proteoforms of urinary prostate-specific antigen by capillary electrophoresis – mass spectrometry

Cited by 19 publications

References 40 publications

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Immunoaffinity Capillary Electrophoresis in the Era of Proteoforms, Liquid Biopsy and Preventive Medicine: A Potential Impact in the Diagnosis and Monitoring of Disease Progression

High sensitivity glycomics in biomedicine

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