Profiling the varietal antioxidative contents and macrochemical composition in Australian faba beans (Vicia faba L.)

Abstract

There is growing interest in pulses such as faba bean for the development of foods with enhanced nutrition, functionality, and health benefits. In this study, seed material from 10 faba bean varieties, grown in replicated field trials in South Australia over consecutive seasons (2016 and 2017), were analysed for ferric reducing antioxidant potential, total phenolics, and total monomeric anthocyanins. Differences in the macrochemical composition of varieties was investigated using attenuated total reflectance mid-infrared spectroscopy. The mean ferric reducing antioxidant potential of the varieties ranged from 237 to 531 mg trolox equivalents 100 g −1 ; the total phenolics from 258 to 571 mg gallic acid equivalents 100 g −1 ; and the total monomeric anthocyanins from 12.7 to 21.0 mg cyanidin-3-glucoside equivalents 100 g −1 . Statistically significant variances in all three measures were found between varieties.Attenuated total reflectance Fourier transformed mid-infrared spectroscopy was found to provide a rapid assessment of the phytochemical composition of the samples. Partial least squares discriminant analysis was able to classify samples by growing year with reasonable accuracy (>87%). There is significant variation in the antioxidant, phenolic, and anthocyanin contents between Australian faba bean varieties. Mid-infrared spectroscopy may prove to be a valuable screening tool for breeders and researchers in the future. K E Y W O R D Santhocyanin, attenuated total reflectance mid-infrared (ATR-MIR) spectroscopy, Fourier transformed infrared (FTIR) spectroscopy, total phenolics

Commercial Lentils (Lens culinaris) Provide Antioxidative and Broad-spectrum Anti-cancerous Effects

“…The median value for all LcCEs was 64.4 mg GAE/g. Although ORAC and TPC are generally correlated in most matrices (Djordjevic et al 2011;Guleria et al 2013;Johnson et al 2020b), no significant correlation between the TEAC and TPC of the LcCEs was observed here (Pearson r = 0.490; p > 0.05). This is likely due to the small sample size (n=5), given that our primary focus was on anticancer activity.…”

Phenolic profiles and nutritional quality of four new mungbean lines grown in northern Australia

“…Extractions and subsequent assays were performed in duplicate. Ferric reducing antioxidant potential (FRAP), cupric reducing antioxidant potential (CUPRAC), total polyphenolic content (TPC) and total monomeric anthocyanin content (TMAC) were determined as previously described (Johnson et al, 2020b). The results for FRAP and CUPRAC were expressed in Trolox equivalents (TEs), TPC results in gallic acid equivalents F I G U R E 1 Location of the growing trial at Home Hill (19.8462 S, 147.2448 E).…”

Nutritional Quality and Bioactive Constituents of Six Australian Plum Varieties

“…For each variety, triplicate extracts were prepared from the corresponding homogenate. To extract the polar compounds, approximately 5 g of plum homogenate was combined with 45 ml of 90% MeOH (v/v), following the method previously reported by our laboratory (Johnson et al, 2020a(Johnson et al, , 2019(Johnson et al, , 2020b. After vortexing briefly, the tube contents were mixed for 60 mins using an end-over-end shaker (Ratek RM4) operating at 50 rpm.…”