Profound differences in IgE and IgG recognition of micro‐arrayed allergens in hyper‐IgE syndromes

Garib, Victoria; Ben-Ali, Meriem; Kundi, Michael; Curin, Mirela; Yaakoubi, Roukaya; Ben‐Mustapha, Imen; Mekki, Najla; Froeschl, Renate; Perkmann, Thomas; Valenta, Rudolf; Barbouche, Mohamed‐Ridha

doi:10.1111/all.15143

Cited by 5 publications

(6 citation statements)

References 42 publications

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“…However, scientists [ 34 , 36 ] also indicate the potential of creating products for AIT to work with Der p 5 and Der p 7 allergens. The efficacy of AIT in patients with sensitization to Der p 5, Der p 7, Der p 20, Der p 21 allergens is actually the subject for further research, and the selection of house dust mite-allergic patients by molecular diagnosis may enhance the success of AIT [ 40 ] and improve the clinical description of patients [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular sensitization pattern to house dust mites is formed from the first years of life and includes group 1, 2, Der p 23, Der p 5, Der p 7 and Der p 21 allergens

et al. 2023

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Background As the process and nature of developing sensitivity to house dust mites (HDMs) remain not fully studied, our goal was to establish the pattern, nature and timeframe of house dust mite (HDM) sensitization development in patients in Ukraine as well as the period when treatment of such patients would be most effective. Methods The data of the multiplex allergy test Alex2 was collected from 20,033 patients. To determine age specifics of sensitization, descriptive statistics were used. Bayesian Network analysis was used to build probabilistic patterns of individual sensitization. Results Patients from Ukraine were most often sensitized to HDM allergens of group 1 (Der p 1, Der f 1) and group 2 (Der p 2, Der f 2) as well as to Der p 23 (55%). A considerable sensitivity to Der p 5, Der p 7 and Der p 21 allergens was also observed. The overall nature of sensitization to HDM allergens among the population of Ukraine is formed within the first year of life. By this time, there is a pronounced sensitization to HDM allergens of groups 1 and 2 as well as to Der p 23. Significance of sensitization to Der p 5, Der p 7 and Der p 21 allergens grows starting from the age of 3–6. Bayesian Network data analysis indicated the leading role of sensitization to Der p 1 and Der f 2. While developing the sensitivity to group 5 allergens, the leading role may belong to Der p 21 allergen. Conclusion The results obtained indicate the importance of determining the sensitization profile using the multi-component approach. A more detailed study of the optimal age for AIT prescription is required as the pattern of sensitization to HDMs is formed during the first year of life.

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Section: Discussionmentioning

confidence: 99%

Molecular sensitization pattern to house dust mites is formed from the first years of life and includes group 1, 2, Der p 23, Der p 5, Der p 7 and Der p 21 allergens

et al. 2023

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“…In these instances, it is critical to detail all phenotypic features, family clinical history and pedigree -at least 3 generations, and all radiographic and laboratory findings. Inheritance patterns, such as a family history significant for consanguinity, for which autosomal recessive inheritance is most consistent, may help narrow the IEI differential diagnosis and may inform patient management decisions 4 . Recently, the International Union of Immunological Societies classified IEI according to common clinical phenotypes, detailing inheritance, genes involved, and other common features of the conditions 2,3 .…”

Section: Discussionmentioning

confidence: 99%

Familial Presentation of Hyper IgE syndrome: A case report

Abdulmohsen

Isa

2022

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“…Basophil degranulation assays were performed with rat basophil leukemia (RBL) cells expressing the human high-affinity receptor for IgE as previously described [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

“…The concentration 10 ng/mL was determined in pilot experiments to be representative for the increasing part of the bell-shaped curve of basophil degranulation by testing concentrations of 100, 10, 1 or 0.1 ng/mL ( Figure S1 ). A concentration of 10 ng/mL of different allergen molecules was also identified in earlier work to represent the increasing part of the bell-shaped basophil degranulation curve using the RBL cell line [ 23 ]. The release of β-hexosaminidase supernatants was analyzed by incubating culture supernatants with 80 μmol/L 4-methylumbelliferyl -N-acetyl-β-D-glucosaminide (Sigma-Aldrich) in citrate buffer (0.1 mol/L, pH 4.5) for 1 h at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Extensively Hydrolyzed Hypoallergenic Infant Formula with Retained T Cell Reactivity

et al. 2022

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Background: Immunoglobulin E (IgE)-mediated cow’s milk allergy (CMA) can be life-threatening and affects up to 3% of children. Hypoallergenic infant formulas based on hydrolyzed cow’s milk protein are increasingly considered for therapy and prevention of cow’s milk allergy. The aim of this study was to investigate the allergenic activity and ability to induce T cell and cytokine responses of an infant formula based on extensively hydrolyzed cow’s milk protein (whey) (eHF, extensively hydrolyzed formula) supplemented with Galactooligosaccharides (GOS) and Limosilactobacillus fermentum CECT5716 (LF) to determine its suitability for treatment and prevention of CMA. Methods: eHF and standard protein formula based on intact cow’s milk proteins (iPF) with or without Galactooligosaccharide (GOS) and Limosilactobacillus fermentum CECT5716 (LF) were investigated with allergen-specific antibodies and tested for IgE reactivity and allergenic activity in basophil degranulation assays with sera from cow’s milk (CM)-allergic infants/children. Their ability to stimulate T cell proliferation and cytokine secretion in cultured peripheral blood mononuclear cells (PBMC) from CM-allergic infants and children was studied with a FACS-based carboxyfluorescein diacetate succinimidyl ester (CFSE) dilution assay and xMAP Luminex fluorescent bead-based technology, respectively. Results: An eHF supplemented with GOS and LF exhibiting almost no IgE reactivity and allergenic activity was identified. This eHF induced significantly lower inflammatory cytokine secretion as compared to an intact protein-based infant formula but retained T cell reactivity. Conclusions: Due to strongly reduced allergenic activity and induction of inflammatory cytokine secretion but retained T cell reactivity, the identified eHF may be used for treatment and prevention of CMA by induction of specific T cell tolerance.

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Profound differences in IgE and IgG recognition of micro‐arrayed allergens in hyper‐IgE syndromes

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri butio n-NonCo mmerc ial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 5 publications

References 42 publications

Molecular sensitization pattern to house dust mites is formed from the first years of life and includes group 1, 2, Der p 23, Der p 5, Der p 7 and Der p 21 allergens

Molecular sensitization pattern to house dust mites is formed from the first years of life and includes group 1, 2, Der p 23, Der p 5, Der p 7 and Der p 21 allergens

Familial Presentation of Hyper IgE syndrome: A case report

Extensively Hydrolyzed Hypoallergenic Infant Formula with Retained T Cell Reactivity

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