Progenitor Cell Isolation from Muscle-derived Cells based on Adhesion Properties

Abstract

Adult skeletal muscle possesses remarkable regenerative capacity that has conventionally been attributed to the satellite cells. These precursor cells were thought to contain distinct populations with varying myogenic potential. Recently, the identification of multipotent stem cells capable of new myofiber formation has expanded the general view on the muscle regenerative process. Here we examined the characteristics of turkey skeletal muscle-derived cell (MDC) populations that were separated according to their adhesion abilities. We sought to determine whether these abilities could be a potential tool for separating cells with different myogenic commitment. Using the preplate technique, we showed that MDCs display a wide range of adhesion ability, allowing us to isolate a marginal fraction with initial adhesion defect. Methodological investigations revealed that this defect represents an intrinsic and well-established biological feature for these cells. In vitro behavioral and morphological analyses showed that late adherent cells (LACs) share several primitive cell characteristics. Phenotypic assessment indicated that LACs contain early stage myogenic cells and immature progenitors of satellite cells, whereas early adherent cells consist mainly of fully committed precursors. Overall, our findings demonstrate for the first time in an avian model that differential MDC adhesion properties could be used to efficiently purify cells with varying myogenic commitment, including immature progenitor cells. This manuscript contains online supplemental material at http://www.jhc.org . Please visit this article online to view these materials.

Isolation of myogenic progenitor populations from Pax7-deficient skeletal muscle based on adhesion characteristics

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The isolation and cultivation of bone marrow stem cells and evaluation of differences for neural-like cells differentiation under the induction with neurotrophic factors

“…Previous studies indicated that direct adherence was the preferential method for MSC isolation and purification (Rouger et al 2007;Yang and Yang 2008). Identification of MSC was mainly based on morphology and functional characteristics.…”

Effect of Mesenchymal Cells on Myoblast Sheets Embedded in Collagen Gel

“…Skeletal muscle tissue, which was separated from the oral mucosal tissue, was treated with 0.125% trypsin at 37°C for 2 hr to achieve cell dissociation 14,15,17) . Initially, the cells contained high amounts of fibroblasts.…”

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