Progenitor cell maturation in the developing vertebrate retina

Yang, Hyun; Silva, Amila O.; Koyano-Nakagawa, Naoko; McLoon, Steven C.

doi:10.1002/dvdy.22116

Cited by 18 publications

(14 citation statements)

References 67 publications

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“…Further supporting this identity, cells in both the apical plate and lateral ectoderm that express SoxC also express Delta (Fig. 1D,E), which is characteristic of neural precursors in embryos (Vässin et al, 1987;Yang et al, 2009). Furthermore, when DeltaNotch signaling is blocked by treatment with DAPT, which is a gamma secretase inhibitor of Notch signaling, SoxC-expressing cells increase in number and form small clusters (Fig.…”

Section: Soxc Function Is Required Downstream Of Six3 For Developmentmentioning

confidence: 60%

Neurogenic gene regulatory pathways in the sea urchin embryo

Wen-ling

Angerer

2015

Development

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During embryogenesis the sea urchin early pluteus larva differentiates 40-50 neurons marked by expression of the panneural marker synaptotagmin B (SynB) that are distributed along the ciliary band, in the apical plate and pharyngeal endoderm, and 4-6 serotonergic neurons that are confined to the apical plate. Development of all neurons has been shown to depend on the function of Six3. Using a combination of molecular screens and tests of gene function by morpholino-mediated knockdown, we identified SoxC and Brn1/2/4, which function sequentially in the neurogenic regulatory pathway and are also required for the differentiation of all neurons. Misexpression of Brn1/2/4 at low dose caused an increase in the number of serotonin-expressing cells and at higher dose converted most of the embryo to a neurogenic epithelial sphere expressing the Hnf6 ciliary band marker. A third factor, Z167, was shown to work downstream of the Six3 and SoxC core factors and to define a branch specific for the differentiation of serotonergic neurons. These results provide a framework for building a gene regulatory network for neurogenesis in the sea urchin embryo.

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Section: Soxc Function Is Required Downstream Of Six3 For Developmentmentioning

confidence: 60%

Neurogenic gene regulatory pathways in the sea urchin embryo

Wen-ling

Angerer

2015

Development

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“…bHLH transcription factors have been shown to be integral during embryonic development, playing roles in myogenesis, heart development, hematopoiesis, and neurogenesis (34). Of the various bHLH transcription factors predicted to bind the Ebox, E2A and NeuroD1 are expressed in developing vertebrate retina (41), and have been reported to play a role in retinal neurogenesis and differentiation (42,43). In these instances, E2A and NeuroD1 are considered to be proneural bHLH proteins, acting as transcriptional activators.…”

Section: Discussionmentioning

confidence: 99%

Genetic modulation of horizontal cell number in the mouse retina

Whitney

Raven

Ciobanu

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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Neuronal populations display conspicuous variability in their size among individuals, but the genetic sources of this variation are largely undefined. We demonstrate a large and highly heritable variation in neuron number within the mouse retina, affecting a critical population of interneurons, the horizontal cells. Variation in the size of this population maps to the distal end of chromosome (Chr) 13, a region homologous to human Chr 5q11.1–11.2. This region contains two genes known to modulate retinal cell number. Using conditional knock-out mice, we demonstrate that one of these genes, the LIM homeodomain gene Islet-1 ( Isl1 ), plays a role in regulating horizontal cell number. Genetic differences in Isl1 expression are high during the period of horizontal cell production, and cis -regulation of Isl1 expression within the retina is demonstrated directly. We identify a single nucleotide polymorphism in the 5′ UTR of Isl1 that creates an E-box sequence as a candidate causal variant contributing to this variation in horizontal cell number.

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“…Previous studies have shown generalized Dll1 expression before retinal neuron differentiation (Haddon et al, 1998;Kay et al, 2005;Nelson and Reh, 2008;Rocha et al, 2009;Yang et al, 2009). We decided to investigate this issue by performing a systematic spatiotemporal analysis in the chick retina to evaluate the expression pattern of Dll1 and other genes involved in the neurogenic process.…”

Section: Dll1 Is Expressed Before the Initiation Of Neurogenesis In Tmentioning

confidence: 98%

“…For instance, in the early zebrafish embryo, strong deltaD expression delineates the whole developing retina a few hours before the initiation of neurogenesis in this tissue (Haddon et al, 1998;Kay et al, 2005). In both the avian and murine retina, Delta-like 1 (Dll1) is expressed more peripherally than its homolog Dll4, being detected in a high proportion of mitotically active progenitor cells (Nelson and Reh, 2008;Rocha et al, 2009;Yang et al, 2009). In Drosophila, Delta (Dl) expression has been shown to precede achaete protein accumulation in microchaeta proneural stripes (Parks et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Regulation of neuronal differentiation at the neurogenic wavefront

et al. 2012

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SUMMARYSignaling mediated by the Delta/Notch system controls the process of lateral inhibition, known to regulate neurogenesis in metazoans. Lateral inhibition takes place in equivalence groups formed by cells having equal capacity to differentiate, and it results in the singling out of precursors, which subsequently become neurons. During normal development, areas of active neurogenesis spread through non-neurogenic regions in response to specific morphogens, giving rise to neurogenic wavefronts. Close contact of these wavefronts with non-neurogenic cells is expected to affect lateral inhibition. Therefore, a mechanism should exist in these regions to prevent disturbances of the lateral inhibitory process. Focusing on the developing chick retina, we show that Dll1 is widely expressed by non-neurogenic precursors located at the periphery of this tissue, a region lacking Notch1, lFng, and differentiation-related gene expression. We investigated the role of this Dll1 expression through mathematical modeling. Our analysis predicts that the absence of Dll1 ahead of the neurogenic wavefront results in reduced robustness of the lateral inhibition process, often linked to enhanced neurogenesis and the presence of morphological alterations of the wavefront itself. These predictions are consistent with previous observations in the retina of mice in which Dll1 is conditionally mutated. The predictive capacity of our mathematical model was confirmed further by mimicking published results on the perturbation of morphogenetic furrow progression in the eye imaginal disc of Drosophila. Altogether, we propose that Notch-independent Delta expression ahead of the neurogenic wavefront is required to avoid perturbations in lateral inhibition and wavefront progression, thus optimizing the neurogenic process.

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Progenitor cell maturation in the developing vertebrate retina

Cited by 18 publications

References 67 publications

Neurogenic gene regulatory pathways in the sea urchin embryo

Neurogenic gene regulatory pathways in the sea urchin embryo

Genetic modulation of horizontal cell number in the mouse retina

Regulation of neuronal differentiation at the neurogenic wavefront

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