Progesterone-induced RNA Hand2os1 regulates decidualization in mice uteri

Jia, Yanni; Cai, Rui; Yu, Tong; Zhang, Ruixue; Liu, Shouqin; Guo, Xinyan; Shang, Chunmei; Wang, Aihua; Jin, Yaping; Lin, Peng

doi:10.1530/rep-19-0401

Cited by 17 publications

(10 citation statements)

References 41 publications

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“…The lncRNA DNM3OS sponged miR-126 to affect cell proliferation and apoptosis by regulating IGF1, by binding to the promoter of miR-126 in CHON-001 cells [64]. The expression level of the lncRNA HAND2OS1 was higher on day 4 of pregnancy than day 1 of pregnancy, while the level increased dramatically on day 6, which is similar to the findings of this study, where two transcripts of the lncRNA HAND2OS1 were downregulated at E0.5 [65]. Ankyrin repeat domain protein (ANKRD) is a large family of proteins including enzymes, transcription factors, and membrane receptors, which are composed of multiple copies of ankyrin repeat (AR) with a 33-amino-acid motif [66].…”

Section: Discussionsupporting

confidence: 88%

Comparison of lncRNA Expression in the Uterus between Periods of Embryo Implantation and Labor in Mice

Zhao

Chen

Cao

et al. 2022

Animals

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Uterine function during pregnancy is regulated mainly by progesterone (P4) and estrogen (E2). Serum P4 levels are known to fluctuate significantly over the course of pregnancy, especially during embryo implantation and labor. In this study, pregnant mice at E0.5, E4.5, E15.5, and E18.5 (n = 3/E) were used for an RNA-Seq-based analysis of mRNA and lncRNA expression. In this analysis, 1971 differentially expressed (DE) mRNAs, 493 known DE lncRNAs, and 1041 novel DE lncRNAs were found between E0.5 and E4.5 at the embryo implantation stage, while 1149 DE mRNAs, 192 known DE lncRNAs, and 218 novel DE lncRNAs were found between E15.5 and E18.5 at the labor stage. The expression level of lncRNA-MMP11 was significantly downregulated by P4 treatment on MSM cells, while lncRNA-ANKRD37 was significantly upregulated. Notably, 117 DE mRNAs, 19 known DE lncRNAs, and 31 novel DE lncRNAs were commonly expressed between the two stages, indicating that these mRNAs and lncRNAs may be directly or indirectly regulated by P4.

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Section: Discussionsupporting

confidence: 88%

Comparison of lncRNA Expression in the Uterus between Periods of Embryo Implantation and Labor in Mice

Zhao

Chen

Cao

et al. 2022

Animals

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“…49 The expression of lncRNA Hand2os1 is induced by P4 in a PR-dependent manner during decidualization, and the use of a PR antagonist abolishes P4-induced Hand2os1 expression. 52 In this study, we found that BMPR1B-AS1 was up-regulated during decidualization in vitro. knockdown of BMPR1B-AS1 inhibited the expression of decidualization marker molecules and reversed the MET process, which prevented hESCs from maintaining the decidualization-specific morphology induced by cAMP and MPA.…”

Section: Discussionmentioning

confidence: 56%

“…In endometrial lncRNA expression profiles, there were differences between patients with recurrent failure of implantation and normal women, suggesting a tight relationship between lncRNA and endometrial receptivity 49 . The expression of lncRNA Hand2os1 is induced by P4 in a PR‐dependent manner during decidualization, and the use of a PR antagonist abolishes P4‐induced Hand2os1 expression 52 . In this study, we found that BMPR1B‐AS1 was up‐regulated during decidualization in vitro.…”

Section: Discussionmentioning

confidence: 56%

Long noncoding RNA BMPR1B‐AS1 stability regulated by IGF2BP2 affects the decidualization in endometriosis patients through the SMAD1/5/9 pathway

Que,

Ren,

Yang

et al. 2024

The FASEB Journal

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Endometriosis (EMs)‐related infertility commonly has decreased endometrial receptivity and normal decidualization is the basis for establishing and maintaining endometrial receptivity. However, the potential molecular regulatory mechanisms of impaired endometrial decidualization in patients with EMs have not been fully clarified. We confirmed the existence of reduced endometrial receptivity in patients with EMs by scanning electron microscopy and quantitative real‐time PCR. Here we identified an lncRNA, named BMPR1B‐AS1, which is significantly downregulated in eutopic endometrium in EMs patients and plays an essential role in decidual formation. Furthermore, RNA pull‐down, mass spectrometry, RNA immunoprecipitation, and rescue analyses revealed that BMPR1B‐AS1 positively regulates decidual formation through interaction with the RNA‐binding protein insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2). Downregulation of IGF2BP2 led to a decreased stability of BMPR1B‐AS1 and inhibition of activation of the SMAD1/5/9 pathway, an inhibitory effect which diminished decidualization in human endometrial stromal cells (hESCs) decidualization. In conclusion, our identified a novel regulatory mechanism in which the IGF2BP2‐BMPR1B‐AS1‐SMAD1/5/9 axis plays a key role in the regulation of decidualization, providing insights into the potential link between abnormal decidualization and infertility in patients with EMs, which will be of clinical significance for the management and treatment of infertility in patients with EMs.

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“…The expression of many lncRNAs is upregulated during decidualization, such as NEAT1 , RP11-627G23.1 , and PSORS1C3 , but their function in this process remains to be elucidated [ 25 ]. These lncRNAs may regulate the expression of genes associated with cell proliferation, differentiation, migration, and angiogenesis during decidualization, as proposed in mice uteri [ 189 ]. PTENP1 , a lncRNA that is highly expressed in the luminal epithelium during the mid-secretory phase, regulates the expression of several miRNAs to promote the implantation process [ 173 ] (Fig.…”

Section: Non-coding Rnasmentioning

confidence: 99%

The role of epigenetic mechanisms in the regulation of gene expression in the cyclical endometrium

et al. 2021

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Background The human endometrium is a highly dynamic tissue whose function is mainly regulated by the ovarian steroid hormones estradiol and progesterone. The serum levels of these and other hormones are associated with three specific phases that compose the endometrial cycle: menstrual, proliferative, and secretory. Throughout this cycle, the endometrium exhibits different transcriptional networks according to the genes expressed in each phase. Epigenetic mechanisms are crucial in the fine-tuning of gene expression to generate such transcriptional networks. The present review aims to provide an overview of current research focused on the epigenetic mechanisms that regulate gene expression in the cyclical endometrium and discuss the technical and clinical perspectives regarding this topic. Main body The main epigenetic mechanisms reported are DNA methylation, histone post-translational modifications, and non-coding RNAs. These epigenetic mechanisms induce the expression of genes associated with transcriptional regulation, endometrial epithelial growth, angiogenesis, and stromal cell proliferation during the proliferative phase. During the secretory phase, epigenetic mechanisms promote the expression of genes associated with hormone response, insulin signaling, decidualization, and embryo implantation. Furthermore, the global content of specific epigenetic modifications and the gene expression of non-coding RNAs and epigenetic modifiers vary according to the menstrual cycle phase. In vitro and cell type-specific studies have demonstrated that epithelial and stromal cells undergo particular epigenetic changes that modulate their transcriptional networks to accomplish their function during decidualization and implantation. Conclusion and perspectives Epigenetic mechanisms are emerging as key players in regulating transcriptional networks associated with key processes and functions of the cyclical endometrium. Further studies using next-generation sequencing and single-cell technology are warranted to explore the role of other epigenetic mechanisms in each cell type that composes the endometrium throughout the menstrual cycle. The application of this knowledge will definitively provide essential information to understand the pathological mechanisms of endometrial diseases, such as endometriosis and endometrial cancer, and to identify potential therapeutic targets and improve women’s health.

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Progesterone-induced RNA Hand2os1 regulates decidualization in mice uteri

Cited by 17 publications

References 41 publications

Comparison of lncRNA Expression in the Uterus between Periods of Embryo Implantation and Labor in Mice

Comparison of lncRNA Expression in the Uterus between Periods of Embryo Implantation and Labor in Mice

Long noncoding RNA BMPR1B‐AS1 stability regulated by IGF2BP2 affects the decidualization in endometriosis patients through the SMAD1/5/9 pathway

The role of epigenetic mechanisms in the regulation of gene expression in the cyclical endometrium

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