Progesterone Injection Assay by Liquid Chromatography

King, Ronald C.; Grady, Lee T.; Reamer, Jeanne T.

doi:10.1002/jps.2600631021

Cited by 25 publications

(3 citation statements)

References 9 publications

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“…21 Other chromatographic parameters, such as capacity factor (k 0 ), resolution (R) tailing factor (T) and theoretical plate number (N), were also analyzed. [22][23][24][25] The capacity factor is a measure of where the peak of interest is located with respect to the void volume, i.e., corresponds to the elution time of the non-retained components. R is a measure of the degree of separation of two peaks.…”

Section: Methods Validationmentioning

confidence: 99%

A rapid reversed-phase HPLC method for the simultaneous analysis of olanzapine and simvastatin in dual nanostructured lipid carriers

2013

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Section: Methods Validationmentioning

confidence: 99%

A rapid reversed-phase HPLC method for the simultaneous analysis of olanzapine and simvastatin in dual nanostructured lipid carriers

2013

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“…All analytical chemists should know that if a response curve has an intercept significantly different from zero, proper provision in the calculation must be made. The conventional, and always correct techniques, whether the function is linear or nonlinear, are graphical interpolation from the plot (3)(4)(5) or the use of an algebraic equation of the function (6,7). These techniques can be quite sophisticated as illustrated in the work of Govindaraju, Mevelle, and Chouard (8) who use computer-stored calibration curves as second-degree polynominals with three regression coefficients for each curve, and in the work of Mitchell et al (1) who employ a multiple-curve procedure which uses a series of least-squares regression equations with confidence bands computed from the calibration data.…”

mentioning

confidence: 99%

“…Wittmer and Haney ( 12) and Won et al (13) state correctly after having shown that the intercept value was insignificantly low, that these data "indicate that the liquid chromatographic method can be used with a single-point standard". Authors such as Sondack ( 14) who states that "the extrapolation results passed through the origin" and King et al (5) who conclude "a standard graph rather than a one-point standard is required for maximum accuracy in the procedure" are examples of those who clearly understand the requirement. Unfortunately, too often authors simply state that the response curve was linear but fail to report whether a significant intercept was found (15)(16)(17)(18).…”

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confidence: 99%

Potential error in single-point-ratio calculations based on linear calibration curves with a significant intercept

Cardone¹,

Palermo²,

Sybrandt³

1980

Anal. Chem.

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Calculations based on a single-point standard linear calibration curve which has a finite intercept are a potential source of bias error. The error has been shown to be a function of magnitude of the slope and intercept, and the magnitude of the difference in the standard and sample responses. For maximum accuracy, the ratio of the difference in responses to the standard response should be the lowest possible. For any response curve, a reliable calculation of the percent error resulting from making a single-point-ratio calculation (SPRC) where a finite intercept exists is shown so that a decision as to its proper use or non-use may be made. In most cases, even when an analytically significant intercept is found in a linear calibration curve, the SPRC technique may stili be employed by calculating the sample signal to standard signal, S,/S, ratio range for an acceptable % error bias range. This calculation of the S,/S, ratio constitutes a trade-off between the use of a single standard and a shortened linear dynamic range with its possibility of more reanalyses against the use of the entire linear dynamic range with its mandatory requirement of more standards but the lesser possibility of reanalyses.

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Simultaneous Separation of Four Types of Steroid Hormones by Micellar Electrokinetic Chromatography with Cetyltrimethylammonium Bromide

Chang

Chiu

Lee

et al. 2008

J Chinese Chemical Soc

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Micellar electrokinetic chromatography (MEKC) using a cationic surfactant, cetyltrimethylammonium bromide (CTAB), was applied to simultaneous separation of four types of hydrophobic steroid hormones including androgens, estrogens, progestins, and glucocorticoids. Various parameters, such as the concentration of CTAB, type and proportion of organic modifiers, and sample matrix have been demonstrated to be important with respect to the separation resolution, analysis time, and repeatability. Successful separation of cortisone, hydrocortisone, estriol, testosterone, estrone, progesterone, and estradiol was achieved at −25 kV using 100 mM Tris‐boric acid buffer (pH 9.0) containing 40 mM CTAB and 20% 2‐propanol using ultraviolet (UV) absorption detection. For estrogen compounds which possess intrinsic fluorescence, detection limits at the sub μM level could be achieved with laser‐induced fluorescence (LIF) detection. Two kinds of pharmaceutical preparations were analyzed using the developed method, and the results were in good agreement with the label claims.

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Progesterone Injection Assay by Liquid Chromatography

Cited by 25 publications

References 9 publications

A rapid reversed-phase HPLC method for the simultaneous analysis of olanzapine and simvastatin in dual nanostructured lipid carriers

A rapid reversed-phase HPLC method for the simultaneous analysis of olanzapine and simvastatin in dual nanostructured lipid carriers

Potential error in single-point-ratio calculations based on linear calibration curves with a significant intercept

Simultaneous Separation of Four Types of Steroid Hormones by Micellar Electrokinetic Chromatography with Cetyltrimethylammonium Bromide

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