Progesterone Levels in Peripheral Plasma During the Luteal Phase of the Normal Human Menstrual Cycle Measured by a Rapid Competitive Protein Binding Technique

Johansson, Elof D. B.

doi:10.1530/acta.0.0610592

Cited by 221 publications

(81 citation statements)

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“…A protein binding method based on that of Johansson (1969) was used. The assay utilized a guinea-pig progesterone-binding protein with the progesterone being measured in O·I-ml samples of plasma.…”

Section: Progesterone Assaymentioning

confidence: 99%

Plasma Progesterone, FSH and LH Levels Associated with Implantation in the Mouse

Gidley-Baird¹

1977

Aust. Jnl. Of Bio. Sci.

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Aust. J. Bioi. Sci., 1977, 30, 289-96 Measurement of plasma progesterone, LH and FSH were made every 6 h during the first 6 days of pregnancy in the mouse. Plasma progesterone and LH were low on day 1, minimum values being recorded at 24 h post coitus. Concentrations of both these hormones started rising during the second half of day 2 with the rise continuing during day 3 to a progesterone peak of 25 ng/ml early on day 4 and an LH peak of 37 ng/mllate on day 4. Levels of progesterone fell during day 4 and LH during day 5 to approximately half their respective peak values and then remained relatively constant over the remainder of the measurement period. Levels of FSH, which were high early on day 1 (180 ng/ml), fell sharply by midday with a small rise late in the day followed by a decline during day 2 to a minimum level of 20 ng/ml at 48 h post coitus. Early on day 3 FSH values rose to 120 ng/ml then fell to 50-60 ng/ml during the next 6 h and remained relatively stable at this level during days 4 and 5. It is suggested that LH is concerned with progesterone production and maintenance of the corpus luteum whilst FSH is concerned with the production of oestrogen required for implantation in this species.

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Section: Progesterone Assaymentioning

confidence: 99%

Plasma Progesterone, FSH and LH Levels Associated with Implantation in the Mouse

Gidley-Baird¹

1977

Aust. Jnl. Of Bio. Sci.

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“…Oestrogens have been shown to possess both luteolytic and luteotrophic properties when given alone, or in association with progestagens (Wiltbank, 1966;Lemon, 1975) and oestradiol benzoate has been used with PRIDs to improve synchronization of oestrus (Roche, 1978). Prostaglandins have also been shown to be effective luteolysins (Cooper, 1974) and have been used to improve the synchronization of oestrus after short treatments with a PRID (Roche, 1976;Hansel & Beai, 1979 Neill, Johansson, Datta & Knobil (1967) and incorporating the modifications of Johansson (1969).…”

Section: Introductionmentioning

confidence: 99%

“…A light petroleum ether of boiling point 40-70°C (May and Baker, Australia) was used which did not extract steroids from plasma in amounts significantly different from those reported by Johansson (1969); 88% ± 1% vs 86% ± 3% (mean ± s.e.m.) recovery of progesterone and 00-0-3% vs 0-0-0-3% (range) recovery of [l,2-3H]corticosterone respectively.…”

Section: Introductionmentioning

confidence: 99%

Effects of progesterone, oestradiol benzoate and cloprostenol on luteal function in the heifer

Munro¹,

Moore²

1985

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“…Plasma progesterone determinations were performed on duplicate o· 5-ml aliquots of plasma using the competitive protein binding method described by Neill et at. (1967) and Johansson (1969).…”

Section: Methodsmentioning

confidence: 98%

Effect of Progesterone and Oestrogen on the Survival and Development of Fertilized Ova in the Ovariectomized Ewe

Trounson¹,

Moore²

1974

Aust. Jnl. Of Bio. Sci.

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Two experiments were carried out to investigate the importance of progesterone and oestrogen for the survival and development of fertilized ova in ovariectomized ewes. In experiment 1 ewes were treated with progesterone (P) alone or progesterone and 17 p-oestradiol (P + E2) following ovariectomy on the second (day 2) or sixth day (day 6) after mating to vasectomized rams. Fertilized ova were transferred to the ewes either immediately after ovariectomy or on day 6 after ovariectomy on day 2. In experiment 2 ewes that had been given an equine anterior pituitary extract to induce multiple ovulation were ovariectomized on day 2 after mating to fertile rams, and then treated with P or P+E2. On day 18 the uteri of all ewes were flushed to recover embryos. Treatment with P commenced immediately after ovariectomy and E2 was given from day 21-to day 5, to ewes ovariectomized on day 2. Ovarian vein blood was collected just prior to ovariectomy and was assayed for its progesterone and oestrogen contents.In both experiments oestradiol had no effect upon the proportion of ewes with normal embryos. In experiment 1 delay of transfer until day 6 after ovariectomy on day 2 increased the proportion of ewes with normal embryos [28 of 43 (65 %) v. 16 of 46 (35 %)]. Transfers on day 6 after ovariectomy on the same day tended to be less successful than transfers on day 6 after ovariectomy on day 2 [11 of 23 (48 %) v. 28 of 43 (65 %)]. In all ewes in experiment 2, and in experiment 1 where ovariectomies and transfers were carried out on the same day, survival and normal development of ova occurred only in ewes in which the ovarian vein progesterone levels at ovariectomy, expressed as progesterone per corpus luteum, fell within precise limits. There was no such relationship in ewes ovariectomized on day 2 and given progesterone prior to transfer on day 6.It would appear that the secretion of oestradiol during the luteal phase of the oestrous cycle in the ewe has little effect on the survival and development of ova. However, progesterone appears to have an important role. Further, it would appear that as early as the second day after oestrus the activity of the corpus luteum, as assessed by its progesterone secretion, may well influence the success or failure of pregnancy.

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Progesterone Levels in Peripheral Plasma During the Luteal Phase of the Normal Human Menstrual Cycle Measured by a Rapid Competitive Protein Binding Technique

Cited by 221 publications

References 0 publications

Plasma Progesterone, FSH and LH Levels Associated with Implantation in the Mouse

Plasma Progesterone, FSH and LH Levels Associated with Implantation in the Mouse

Effects of progesterone, oestradiol benzoate and cloprostenol on luteal function in the heifer

Effect of Progesterone and Oestrogen on the Survival and Development of Fertilized Ova in the Ovariectomized Ewe

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