Prognostic biomarkers and molecular pathways mediating Helicobacter pylori–induced gastric cancer: a network-biology approach

Abstract

Cancer of the stomach is the second most frequent cancer-related death worldwide. The survival rate of patients with gastric cancer (GC) remains fragile. There is a requirement to discover biomarkers for prognosis approaches. Helicobacter pylori in the stomach is closely associated with the progression of GC. We identified the genes associated with poor/favorable prognosis in H. pylori–induced GC. Multivariate statistical analysis was applied on the Gene Expression Omnibus (GEO) dataset GSE54397 to identify differentially expressed miRNAs (DEMs) in gastric tissues with H. pylori–induced cancer compared with the H. pylori–positive with non-cancerous tissue. A protein interaction map (PIM) was built and subjected to DEMs targets. The enriched pathways and biological processes within the PIM were identified based on substantial clusters. Thereafter, the most critical genes in the PIM were illustrated, and their prognostic impact in GC was investigated. Considering p-value less than 0.01 and |Log2 fold change| as >1, five microRNAs demonstrated significant changes among the two groups. Gene functional analysis revealed that the ubiquitination system, neddylation pathway, and ciliary process are primarily involved in H. pylori–induced GC. Survival analysis illustrated that the overexpression of DOCK4, GNAS, CTGF, TGF-b1, ESR1, SELE, TIMP3, SMARCE1, and TXNIP was associated with poor prognosis, while increased MRPS5 expression was related to a favorable prognosis in GC patients. DOCK4, GNAS, CTGF, TGF-b1, ESR1, SELE, TIMP3, SMARCE1, TXNIP, and MRPS5 may be considered prognostic biomarkers for H. pylori–induced GC. However, experimental validation is necessary in the future.

Overexpression of heat shock protein 47 is associated with increased proliferation and metastasis in gastric cancer

“…Multiple genes are associated with gastric tumorigenesis [ 17 , 18 ]. In an effort to identify the genes responsible for the tumorigenesis of GC, we performed a genome-wide gene expression analysis of human GC and adjacent normal tissues from 27 Korean GC patients (NCBI GEO accession GSE30727) [ 19 ].…”

Lactobacillus plantarum associated with antimicrobial therapy in the eradication of Helicobacter pylori: a randomized clinical trial

“…Recent studies have shown that eradicating H. pylori in infected asymptomatic individuals of all ages can reduce the occurrence of gastric cancer (YU et al, 2023;KAMAREHEI et al, 2023). Antibiotic therapy is the most effective and widely used treatment for H. pylori infection, in which clarithromycin, amoxicillin, or metronidazole can inhibit H. pylori infection (GODERSKA et al, 2018).…”