Prognostic circulating proteomic biomarkers in colorectal liver metastases

Kim, Dongchan; Gupta, Bhavya; Wong, Geoffrey Yuet Mun

doi:10.1016/j.csbj.2023.03.011

Cited by 5 publications

(4 citation statements)

References 46 publications

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“…The mechanisms of melanoma metastasis have been the subject of extensive studies for decades, as melanoma has one of the highest metastatic potentials among human cancers with diverse secondary tumor sites, including the liver [15,26,27]. However, most of the recent studies are focusing on the most common primary cancers that metastasize to the liver, including colorectal and pancreatic cancers as well as uveal melanoma [16,[28][29][30]. The organotropism of the liver is influenced by different factors such as blood flow pattern, tumor stage, and histological subtype of the tumor [18].…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Changes in Cytokine and Chemokine Receptors in Association with Melanoma Liver Metastasis

Koroknai

Szász

Balázs

2023

IJMS

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Cytokines and chemokines (chemotactic cytokines) are soluble extracellular proteins that bind to specific receptors and play an integral role in the cell-to-cell signaling network. In addition, they can promote the homing of cancer cells into different organs. We investigated the potential relationship between human hepatic sinusoidal endothelial cells (HHSECs) and several melanoma cell lines for the expression of chemokine and cytokine ligands and receptor expression during the invasion of melanoma cells. In order to identify differences in gene expression related to invasion, we selected invasive and non-invasive subpopulations of cells after co-culturing with HHSECs and identified the gene expression patterns of 88 chemokine/cytokine receptors in all cell lines. Cell lines with stable invasiveness and cell lines with increased invasiveness displayed distinct profiles of receptor genes. Cell lines with increased invasive capacity after culturing with conditioned medium showed a set of receptor genes (CXCR1, IL1RL1, IL1RN, IL3RA, IL8RA, IL11RA, IL15RA, IL17RC, and IL17RD) with significantly different expressions. It is very important to emphasize that we detected significantly higher IL11RA gene expression in primary melanoma tissues with liver metastasis as well, compared to those without metastasis. In addition, we assessed protein expression in endothelial cells before and after co-culturing them with melanoma cell lines by applying chemokine and cytokine proteome arrays. This analysis revealed 15 differentially expressed proteins (including CD31, VCAM-1, ANGPT2, CXCL8, and CCL20) in the hepatic endothelial cells after co-culture with melanoma cells. Our results clearly indicate the interaction between liver endothelial and melanoma cells. Furthermore, we assume that overexpression of the IL11RA gene may play a key role in organ-specific metastasis of primary melanoma cells to the liver.

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Section: Discussionmentioning

confidence: 99%

Gene Expression Changes in Cytokine and Chemokine Receptors in Association with Melanoma Liver Metastasis

Koroknai

Szász

Balázs

2023

IJMS

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“…The mechanisms of melanoma metastasis have been the subject of extensive studies for decades, as melanoma has one of the highest metastatic potential among human cancers with diverse secondary tumor sites including liver [14,25,26]. However, most of the recent studies are focusing on the most common primary cancers that metastasizes to the liver, including colorectal-and pancreatic cancers, as well as uveal melanoma [15,[27][28][29]. The organotropism of the liver is influenced by different factors such as blood flow pattern, tumor stage and histological subtype of the tumor [17].…”

Section: Discussionmentioning

confidence: 99%

Association between <em>IL11RA</em> Overexpression and the Initiation of Melanoma Liver Metastasis

Koroknai¹,

Szász²,

Balázs³

2023

Preprint

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Cytokines and chemokines (chemotactic cytokines) are soluble extracellular proteins that bind to specific receptors and play an integral role in the cell-to-cell signaling network, in addition can promote the homing of cancer cells into different organs. We investigated the potential relationship between human hepatic sinusoidal endothelial cells (HHSECs) and several melanoma cell lines for the expression of chemokine and cytokine ligand and receptor expression during invasion of melanoma cells. In order to define the invasion related gene expression differences, we selected invasive and non-invasive subpopulations of cells after co-culturing with HHSECs. In addition, we determined protein expression of the endothelial cells before and after co-culturing with melanoma cell lines originated from primary tumors. Cell lines with increased invasive capacity after culturing with conditioned medium showed a set of receptor genes (CXCR1, IL1RL1, IL1RN, IL3RA, IL8RA, IL11RA, IL15RA, IL17RC, and IL17RD) with significantly different expression. It is very important note, that the IL11RA gene expression level was also significantly increased in primary melanoma tissues with liver metastasis as well. Proteome arrays revealed 15 differentially expressed proteins (including CD31, VCAM-1, ANGPT2, CXCL8, and CCL20) in the hepatic endothelial cells after co-cultured with melanoma cells. Our findings clearly indicate the interaction between liver endothelial- and melanoma cells. Based on our data, we assume that overexpression of the IL11RA gene might has key role in the formation of organ specific metastasis to the liver by primary melanoma cells.

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“…This information helps clinicians tailor treatment plans to individual patients, enhancing the prospects for successful outcomes. For example, proteomic studies have revealed biomarkers associated with resistance to certain targeted therapies, such as those directed at the epidermal growth factor receptor (EGFR), guiding the selection of more effective treatment options. , In addition to diagnosis and prognosis, proteomic approaches contribute to a deeper understanding of the underlying biology of colorectal cancer. They shed light on the molecular pathways, protein–protein interactions, and post-translational modifications that govern cancer cell behavior.…”

Section: Molecular Profiling Of Colorectal Cancermentioning

confidence: 99%

Advances in Precision Medicine Approaches for Colorectal Cancer: From Molecular Profiling to Targeted Therapies

Kiran,

Yashaswini,

Maheshwari

et al. 2024

ACS Pharmacol. Transl. Sci.

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Precision medicine is transforming colorectal cancer treatment through the integration of advanced technologies and biomarkers, enhancing personalized and effective disease management. Identification of key driver mutations and molecular profiling have deepened our comprehension of the genetic alterations in colorectal cancer, facilitating targeted therapy and immunotherapy selection. Biomarkers such as microsatellite instability (MSI) and DNA mismatch repair deficiency (dMMR) guide treatment decisions, opening avenues for immunotherapy. Emerging technologies such as liquid biopsies, artificial intelligence, and machine learning promise to revolutionize early detection, monitoring, and treatment selection in precision medicine. Despite these advancements, ethical and regulatory challenges, including equitable access and data privacy, emphasize the importance of responsible implementation. The dynamic nature of colorectal cancer, with its tumor heterogeneity and clonal evolution, underscores the necessity for adaptive and personalized treatment strategies. The future of precision medicine in colorectal cancer lies in its potential to enhance patient care, clinical outcomes, and our understanding of this intricate disease, marked by ongoing evolution in the field. The current reviews focus on providing in-depth knowledge on the various and diverse approaches utilized for precision medicine against colorectal cancer, at both molecular and biochemical levels.

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Prognostic circulating proteomic biomarkers in colorectal liver metastases

Cited by 5 publications

References 46 publications

Gene Expression Changes in Cytokine and Chemokine Receptors in Association with Melanoma Liver Metastasis

Gene Expression Changes in Cytokine and Chemokine Receptors in Association with Melanoma Liver Metastasis

Association between <em>IL11RA</em> Overexpression and the Initiation of Melanoma Liver Metastasis

Advances in Precision Medicine Approaches for Colorectal Cancer: From Molecular Profiling to Targeted Therapies

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