Prognostic implication of Ecto-5′-nucleotidase activity in acute lymphoblastic leukemia

Abstract

Ecto-5'-nucleotidase (5'-N) activity was determined in 191 patients (71 children and 120 adults) with acute leukemia. Elevated values for 5'-N were registered in common acute lymphoblastic leukemia (ALL), but blast cells of T-cell ALL (T-ALL) and common ALL antigen-negative non-T-ALL had low enzyme activity comparable with the values of acute non-lymphocytic leukemia. Dependence of remission duration on 5'-N activity was analyzed in 74 adults with ALL, treated similarly in a prospective multicenter trial. The remission curves for ALL patients with 5'-N activity lower than 10 nmol/h x 10(6) cells were substantially and significantly better than those of patients with high activity (greater than 10 nmol/h x 10(6) cells). This difference was also evident in the immunologic subclass common ALL. Statistical evaluation showed that an interaction between immunologic subtype of the blast cells and their 5'-N activity had prognostic significance for remission duration. In addition to the independent factor, initial age, this interaction was also prognostic for survival.

Ecto‐5'‐nucleotidase (CD73) in multidrug‐resistant cell lines generated by doxorubicin

“…The expression of adherence molecules on malignant cells has been reported to influence their resistance to drugs (Graham et al, 1993) and their ability to metastasize (Rudy et al, 1993). In addition to an elevated threshold for sensitivity to some cytostatic drugs, ecto-S'NT+ MDR cells may also display different metastatic behavior and thus may contribute to a poorer prognosis of the disease as shown in clinical studies by Gutensohn and Thiel, (1990) and Pieters et al (1992).…”

“…Several clinical studies have tried to establish a prognostic significance for the expression of ectoJ'NT in malignant cells. Gutensohn and Thiel (1990) found better remission duration for leukemic patients whose cells had low ecto-5'NT activities. Pieters et al (1992) reported similar findings and demonstrated in their study that the level of ecto-5'NT activity of blast cells and their in vitro resistance to 6-thioguanine were not related.…”

Tissue-specific Regulation of the Ecto-5′-nucleotidase Promoter

“…CAT and ␤-Galactosidase Assays-Chloramphenicol acetyltransferase activity was measured at 37°C in a reaction medium containing 1 mM dibutyryl CoA (25 g/assay), 10 M D-threo[dichloroacetyl- [1][2][3][4][5][6][7][8][9][10][11][12][13][14] C]chloramphenicol (56 mCi/mmol, 0.25 Ci/assay) in 0.25 M Tris, pH 8.0, buffer. The incubation was initiated by the addition of 2-50 l of cell extract in a total volume of 125 l and terminated after 1-6 h with the addition of 300 l of xylenes.…”

“…In contrast, elevated activity of ecto-5Ј-NT has been found in breast carcinoma (11), gastric cancer (12), pancreatic cancer (13), chronic myelogenous leukemia (10,14), cutaneous T-cell lymphoma (15), and Walker 256 carcinoma (16). Human renal carcinoma cell lines induced to differentiate by butyric acid have demonstrated both an increase (Cur cell line) and a decrease (Caki cell line) in ecto-5Ј-NT activity (17), demonstrating a complex, and perhaps context-dependent, pattern of ecto-5Ј-NT expression in differentiation and malignant transformation.…”

“…The activity of ecto-5Ј-nucleotidase, although variable, is frequently decreased as a result of neoplastic transformation of cells of hematopoietic origin (10). In contrast, elevated activity of ecto-5Ј-NT has been found in breast carcinoma (11), gastric cancer (12), pancreatic cancer (13), chronic myelogenous leukemia (10,14), cutaneous T-cell lymphoma (15), and Walker 256 carcinoma (16).…”